<i>MfOfd1</i> is crucial for stress responses and virulence in the peach brown rot fungus <i>Monilinia fructicola</i>

Zhang, Mingming; Wang, Zuo-Qian; Xu, Xiao; Huang, Song; Yin, Wei-Xiao; Luo, Chaoxi

doi:10.1111/mpp.12933

Cited by 10 publications

(4 citation statements)

References 60 publications

(69 reference statements)

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“…In the case of important diseases such as Monilinia fructicola fungus, in Prunus species some studies are being focused. To date, pathogenic and genomic studies are scarce in this disease, but recent advances have identified a redox-related gene ( MfOfd1 ) being up-regulated after its inoculation [ 134 ]. In this work, CRISPR/Cas9 was used combined with homologous recombination in order to determine the role of MfOfd1 gene.…”

Section: New Molecular Perspectives In the Postgenomic Eramentioning

confidence: 99%

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus

Prudencio

Devin

Mahdavi

et al. 2022

IJMS

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Mutation is a source of genetic diversity widely used in breeding programs for the acquisition of agronomically interesting characters in commercial varieties of the Prunus species, as well as in the rest of crop species. Mutation can occur in nature at a very low frequency or can be induced artificially. Spontaneous or bud sport mutations in somatic cells can be vegetatively propagated to get an individual with the mutant phenotype. Unlike animals, plants have unlimited growth and totipotent cells that let somatic mutations to be transmitted to the progeny. On the other hand, in vitro tissue culture makes it possible to induce mutation in plant material and perform large screenings for mutant’s selection and cleaning of chimeras. Finally, targeted mutagenesis has been boosted by the application of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 and Transcription activator-like effector nuclease (TALEN) editing technologies. Over the last few decades, environmental stressors such as global warming have been threatening the supply of global demand for food based on population growth in the near future. For this purpose, the release of new varieties adapted to such changes is a requisite, and selected or generated Prunus mutants by properly regulated mechanisms could be helpful to this task. In this work, we reviewed the most relevant mutations for breeding traits in Prunus species such as flowering time, self-compatibility, fruit quality, and disease tolerance, including new molecular perspectives in the present postgenomic era including CRISPR/Cas9 and TALEN editing technologies.

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Section: New Molecular Perspectives In the Postgenomic Eramentioning

confidence: 99%

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus

Prudencio

Devin

Mahdavi

et al. 2022

IJMS

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“…CRISPR-Cas9 ribonucleoproteins have been used to target virulence-related genes to combat M. oryzae, which seriously damage global rice production (Foster et al 2018). Zhang et al (2020) knocked out the SCRE1 gene of Ustilaginoidea virens, thereby signi cantly reducing its virulence in rice. Schuster et al (2017) used CRISPR/Cas9 technology to knock out ve EFF1 genes in U. maydis at the same time, signi cantly reducing the virulence of the strain.…”

Section: Detection and Treatment Of Fungal Infectionsmentioning

confidence: 99%

Applications of CRISPR/Cas Gene-Editing Technology in Fungi

Liao

Cheng

Zhou

et al. 2021

Preprint

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Genome editing technology develop fast in recent years. The traditional gene-editing methods, including homologous recombination, zinc finger endonuclease, and transcription activator-like effector nuclease and so on, which have greatly promoted the research of genetics and molecular biology, have gradually showed their limitations such as low efficiency, high error rate, and complex design. In 2012,a new gene-editing technology, the CRISPR/Cas9 system, was setup based on the research of the immune responses to viruses from archaea and bacteria. Due to its advantages of high target efficiency, simple primer design, and wide application, CRISPR/Cas9 system, whose developers are awared the Nobel Prize in Chemistry this year, has become the dominant genomic editing technology in global academia and some pharmaceuticals. Here we briefly introduce the CRISPR/Cas system and its main applications in yeast, filamentous fungi and macrofungi, including single nucleotide, polygene and polyploid editing, yeast chromosome construction, yeast genome and yeast library construction, CRISPRa/CRISPRi-mediated, CRISPR platform of non-traditional yeast and regulation of metabolic pathway, to highlight the possible applications on fungal infection treatment and to promote the transformation and application of the CRISPR/Cas system in fungi.

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“…1,2 Besides peaches, the host range of M. fructicola is wide, mainly including stone fruits such as plum, apricot, cherry and plum, and other pome fruits. 3 Brown rot severely decreases the yield and quality of nectarines products. 4 Obi et al (2018) reported that >50% of the global post-harvest peach losses are a consequence of brown rot disease.…”

Section: Introductionmentioning

confidence: 99%

“…Monilia fructicola is the causal agent of brown rot, causing major pre‐ and post‐harvest losses in peach production 1,2 . Besides peaches, the host range of M. fructicola is wide, mainly including stone fruits such as plum, apricot, cherry and plum, and other pome fruits 3 . Brown rot severely decreases the yield and quality of nectarines products 4 .…”

Section: Introductionmentioning

confidence: 99%

Resistance to the DMI fungicide mefentrifluconazole in Monilinia fructicola: risk assessment and resistance basis analysis

Li,

Zhang,

Wang

et al. 2023

Pest Management Science

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BACKGROUNDBrown rot disease, caused by Monilinia fructicola, poses a significant challenge to peach production in China. The efficacy of mefentrifluconazole, a new triazole fungicide, in controlling brown rot in peaches has been remarkable. However, the resistance risk and mechanism associated with this fungicide remain unclear. This study was designed to assess the resistance risk of M. fructicola to mefentrifluconazole and reveal the potential resistance mechanism.RESULTSThe mean median effective concentration (EC50) of 101 M. fructicola isolates to mefentrifluconazole was 0.003 μg mL−1, and the sensitivity exhibited a unimodal distribution. Seven mefentrifluconazole‐resistant mutants were generated from three parental isolates in the laboratory through fungicide adaption. The biological characteristics of the resistant mutants revealed that three of them exhibited enhanced survival fitness compared to the parental isolates, whereas the remaining four mutants displayed reduced survival fitness. Mefentrifluconazole showed strong positive cross‐resistance with fenbuconazole, whereas no cross‐resistance was observed with pyrimethanil, procymidone or pydiflumetofen. No overexpression of MfCYP51 gene was detected in the resistant mutants. Multiple sequence alignment revealed that three resistant mutants (MXSB2‐2, Mf12‐1 and Mf12‐2) had a point mutation (G461S) in MfCYP51 protein. Molecular docking techniques confirmed the contribution of this point mutation to mefentrifluconazole resistance.CONCLUSIONThe risk of M. fructicola developing resistance to mefentrifluconazole is relatively low‐to‐medium and point mutation G461S in MfCYP51 could confer mefentrifluconazole resistance in M. fructicola. This study provided essential data for monitoring the emergence of resistance and developing resistance management strategies for mefentrifluconazole. © 2023 Society of Chemical Industry.

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MfOfd1 is crucial for stress responses and virulence in the peach brown rot fungus Monilinia fructicola

Cited by 10 publications

References 60 publications

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus

Spontaneous, Artificial, and Genome Editing-Mediated Mutations in Prunus

Applications of CRISPR/Cas Gene-Editing Technology in Fungi

Resistance to the DMI fungicide mefentrifluconazole in Monilinia fructicola: risk assessment and resistance basis analysis

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