<i>Methylobacillus flagellatus</i> KT contains a novel <i>cbo</i>‐type cytochrome oxidase

Strom, E. V.; Dinarieva, T. Yu.; Netrusov, A. I.

doi:10.1016/s0014-5793(01)02795-8

Cited by 11 publications

(3 citation statements)

References 17 publications

(19 reference statements)

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“…The binding of gaseous ligands to the low-potential heme in CcoP appears then to be a competition between the binding of the exogenous ligand and endogeneous coordination by a local amino acid side chain. It is interesting to note that CO binding to a c-type cytochrome has also been reported in the cbo-type oxidase of Methylobacillus flagellatus, a member of the HCO superfamily that contains a c-type heme and heme O-Cu B dinuclear center (34). However, it is probably not a general characteristic of this family of enzymes, since there is no indication of CO ligation to the reduced heme C present in cytochrome caa 3 oxidase of Bacillus subtilis (35).…”

Section: Spectroscopic Changes Associated With Co Binding Tomentioning

confidence: 92%

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

2003

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Cytochrome cbb(3) oxidase, from Pseudomonas stutzeri, contains a total of five hemes, two of which, a b-type heme in the active site and a hexacoordinate c-type heme, can bind CO in the reduced state. By comparing the cbb(3) oxidase complex and the isolated CcoP subunit, which contains the ligand binding bishistidine-coordinated c-type heme, we have deconvoluted the contribution made by each center to CO binding. A combination of rapid mixing and flash photolysis experiments, coupled with computer simulations, reveals the kinetics of the reaction of c-type heme with CO to be complex as a result of the need to displace an endogenous axial ligand, a property shared with nonsymbiotic plant hemoglobins and some heme-based gas sensing domains. The recombination of CO with heme b(3), unlike all other heme-copper oxidases, including mitochondrial cytochrome c oxidase, is independent of ligand concentration. This observation suggests a very differently organized dinuclear center in which CO exchange between Cu(B) and heme b(3) is significantly enhanced, perhaps reflecting an important determinant of substrate affinity.

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Section: Spectroscopic Changes Associated With Co Binding Tomentioning

confidence: 92%

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

2003

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“…Methylobacillus sp. has been detected in riparian wetlands of Jiuduansha which could produce several oxidases that oxidize diverse compounds ( Strom, Dinarieva & Netrusov, 2001 ; Hu et al, 2014 ). Moreover, Khan et al (2015) proposed that the Methylophilus could be used as a candidate for explosive detoxification in a nitrogen-deficient environment.…”

Section: Discussionmentioning

confidence: 99%

Short-term effects of CO₂ leakage on the soil bacterial community in a simulated gas leakage scenario

Zhang

et al. 2017

PeerJ

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The technology of carbon dioxide (CO2) capture and storage (CCS) has provided a new option for mitigating global anthropogenic emissions with unique advantages. However, the potential risk of gas leakage from CO2 sequestration and utilization processes has attracted considerable attention. Moreover, leakage might threaten soil ecosystems and thus cannot be ignored. In this study, a simulation experiment of leakage from CO2 geological storage was designed to investigate the short-term effects of different CO2 leakage concentration (from 400 g m−2 day−1 to 2,000 g m−2 day−1) on soil bacterial communities. A shunt device and adjustable flow meter were used to control the amount of CO2 injected into the soil. Comparisons were made between soil physicochemical properties, soil enzyme activities, and microbial community diversity before and after injecting different CO2 concentrations. Increasing CO2 concentration decreased the soil pH, and the largest variation ranged from 8.15 to 7.29 (p < 0.05). Nitrate nitrogen content varied from 1.01 to 4.03 mg/Kg, while Olsen-phosphorus and total phosphorus demonstrated less regular downtrends. The fluorescein diacetate (FDA) hydrolytic enzyme activity was inhibited by the increasing CO2 flux, with the average content varying from 22.69 to 11.25 mg/(Kg h) (p < 0.05). However, the increasing activity amplitude of the polyphenol oxidase enzyme approached 230%, while the urease activity presented a similar rising trend. Alpha diversity results showed that the Shannon index decreased from 7.66 ± 0.13 to 5.23 ± 0.35 as the soil CO2 concentration increased. The dominant phylum in the soil samples was Proteobacteria, whose proportion rose rapidly from 28.85% to 67.93%. In addition, the proportion of Acidobacteria decreased from 19.64% to 9.29% (p < 0.01). Moreover, the abundances of genera Methylophilus, Methylobacillus, and Methylovorus increased, while GP4, GP6 and GP7 decreased. Canonical correlation analysis results suggested that there was a correlation between the abundance variation of Proteobacteria, Acidobacteria, and the increasing nitrate nitrogen, urease and polyphenol oxidase enzyme activities, as well as the decreasing FDA hydrolytic enzyme activity, Olsen-phosphorus and total phosphorus contents. These results might be useful for evaluating the risk of potential CO2 leakages on soil ecosystems.

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“…oryzae injects transcription activator-like (TAL) effector proteins into plant host cells to modulate gene expression and thereby the plant response. Among the induced targets are sugars transporter (SWEET) genes, for example OsSWEET11 and OsSWEET14 in leaves (Strom et al, 2001;Baker et al, 2003;Wilkins et al, 2015), which may lead to increase of the sugar levels in the apoplast serving as carbon source for the pathogen. Partially these mechanisms appear to occur also in roots: OsSWEET11 and potential phosphate transporter encoding Os06g29790, which are only moderately induced in leaves (~10/2 fold) (Cernadas et al, 2014), were strongly induced in roots (70 fold, Table S3).…”

Section: Putative Metabolic Responses Affected By Endophyte and Pathogenmentioning

confidence: 99%

Jasmonic Acid, Not Salicyclic Acid Restricts Endophytic Root Colonization of Rice

2020

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Research on the interaction between the non-nodule-forming bacterial endophytes and their host plants is still in its infancy. Especially the understanding of plant control mechanisms which govern endophytic colonization is very limited. The current study sets out to determine which hormonal signaling pathway controls endophytic colonization in rice, and whether the mechanisms deviate for a pathogen. The endophyte Azoarcus olearius BH72-rice model was used to investigate root responses to endophytes in comparison to the recently established pathosystem of rice blight Xanthomonas oryzae pv. oryzae PXO99 (Xoo) in flooded roots. In the rice root transcriptome, 523 or 664 genes were found to be differentially expressed in response to Azoarcus or Xoo colonization, respectively; however, the response was drastically different, with only 6% of the differentially expressed genes (DEGs) overlapping. Overall, Xoo infection induced a much stronger defense reaction than Azoarcus colonization, with the latter leading to down-regulation of many defense related DEGs. Endophyte-induced DEGs encoded several enzymes involved in phytoalexin biosynthesis, ROS (reactive oxygen species) production, or pathogenesis-related (PR) proteins. Among putative plant markers related to signal transduction pathways modulated exclusively during Azoarcus colonization, none overlapped with previously published DEGs identified for another rice endophyte, Azospirillum sp. B510. This suggests a large variation in responses of individual genotypic combinations. Interestingly, the DEGs related to jasmonate (JA) signaling pathway were found to be consistently activated by both beneficial endophytes. In contrast, the salicylate (SA) pathway was activated only in roots infected by the pathogen. To determine the impact of SA and JA production on root colonization by the endophyte and the pathogen, rice mutants with altered hormonal responses were employed: mutant cpm2 deficient in jasmonate synthesis, and RNA interference (RNAi) knockdown lines of NPR1 decreased in salicylic acid-mediated defense responses (NPR1-kd). Only in cpm2, endophytic colonization of Azoarcus was significantly increased, while Xoo colonization was not affected. Surprisingly, NPR1-kd lines showed slightly decreased colonization by Xoo, contrary to published results for leaves. These outcomes suggest that JA but not SA

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Methylobacillus flagellatus KT contains a novel cbo‐type cytochrome oxidase

Cited by 11 publications

References 17 publications

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

Short-term effects of CO₂ leakage on the soil bacterial community in a simulated gas leakage scenario

Jasmonic Acid, Not Salicyclic Acid Restricts Endophytic Root Colonization of Rice

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Methylobacillus flagellatus KT contains a novel cbo‐type cytochrome oxidase

Cited by 11 publications

References 17 publications

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb3 Oxidase from Pseudomonas stutzeri

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb3 Oxidase from Pseudomonas stutzeri

Short-term effects of CO2 leakage on the soil bacterial community in a simulated gas leakage scenario

Jasmonic Acid, Not Salicyclic Acid Restricts Endophytic Root Colonization of Rice

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Product

Resources

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Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

Complex Interactions of Carbon Monoxide with Reduced Cytochrome cbb₃ Oxidase from Pseudomonas stutzeri

Short-term effects of CO₂ leakage on the soil bacterial community in a simulated gas leakage scenario