<i>Medicago truncatula</i>
            : local response of the root nodule proteome to drought stress

González, Esther M.; Wienkoop, Stefanie; Staudinger, Christiana; Lyon, David; Gil‐Quintana, Erena

doi:10.1002/9781119409144.ch142

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…Moreover, recently, we applied the same PEG-based aqueous model to the study of the alterations in pea nodule proteome induced by osmotic stress [ 12 ]. Interestingly, the resulted stress-related patterns of differential protein expression were in good agreement with the previously published data of Gonzalez et al [ 40 ], although the authors used a much more sophisticated and physiologically straightforward split-root approach. Based on this fact and keeping in mind our earlier osmotic stress experiments with mature pea plants at the stage of seed filling [ 39 ], we decided for this model as the most suitable for the analysis of carbonyl metabolome dynamics.…”

Section: Discussionsupporting

confidence: 88%

Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

Soboleva

Frolova

Bureiko

et al. 2022

IJMS

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Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultimately accompanies plants’ response to water deficit. Indeed, enhanced formation of reactive oxygen species in root nodules might result in up-regulation of lipid peroxidation and overproduction of reactive carbonyl compounds (RCCs), which readily modify biomolecules and disrupt cell functions. Thus, the knowledge of the nodule carbonyl metabolome dynamics is critically important for understanding the drought-related losses of nitrogen fixation efficiency and plant productivity. Therefore, here we provide, to the best of our knowledge, for the first time a comprehensive overview of the pea root nodule carbonyl metabolome and address its alterations in response to polyethylene glycol-induced osmotic stress as the first step to examine the changes of RCC patterns in drought treated plants. RCCs were extracted from the nodules and derivatized with 7-(diethylamino)coumarin-3-carbohydrazide (CHH). The relative quantification of CHH-derivatives by liquid chromatography-high resolution mass spectrometry with a post-run correction for derivative stability revealed in total 194 features with intensities above 1 × 105 counts, 19 of which were down- and three were upregulated. The upregulation of glyceraldehyde could accompany non-enzymatic conversion of glyceraldehyde-3-phosphate to methylglyoxal. The accumulation of 4,5-dioxovaleric acid could be the reason for down-regulation of porphyrin metabolism, suppression of leghemoglobin synthesis, inhibition of nitrogenase and degradation of legume-rhizobial symbiosis in response to polyethylene glycol (PEG)-induced osmotic stress effect. This effect needs to be confirmed with soil-based drought models.

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Section: Discussionsupporting

confidence: 88%

Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

Soboleva

Frolova

Bureiko

et al. 2022

IJMS

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Split-root assays for studying legume–rhizobia symbioses, rhizodeposition, and belowground nitrogen transfer in legumes

Thilakarathna

Cope

2021

Journal of Experimental Botany

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Split-root assays have been used widely in studies focused on understanding the complex regulatory mechanisms in legume–rhizobia symbioses, root nitrogen rhizodeposition, and belowground nitrogen transfer, and the effects of different biotic/abiotic factors on this symbiotic interaction. This assay allows a plant to have a root system that is physically divided into two distinct sections that are both still attached to a common shoot. Thus, each root section can be treated separately to monitor local and systemic plant responses. Different techniques are used to establish split-root assemblies, including double-pot systems, divided growth pouches, elbow root assembly, twin-tube systems, a single pot or chamber with a partition in the center, and divided agar plates. This review is focused on discussing the various types of split-root assays currently used in legume-based studies, and their associated advantages and limitations. Furthermore, this review also focuses on how split-root assays have been used for studies on nitrogen rhizodeposition, belowground nitrogen transfer, systemic regulation of nodulation, and biotic and abiotic factors affecting legume–rhizobia symbioses.

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Protein glycation and drought response of pea (<em>Pisum sativum</em> L.) root nodule proteome: a proteomics approach

Shumilina

Gorbach

Popova

et al. 2021

BioComm

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Because of ongoing climate change, drought is becoming the major factor limiting productivity of all plants, including legumes. As these protein-rich crops form symbiotic associations with rhizobial bacteria — root nodules — they readily lose their productivity under drought conditions. Understanding the underlying molecular mechanisms might give access to new strategies to preserve the productivity of legume crops under dehydration. As was shown recently, development of drought response is accompanied by alterations in the patterns of protein glycation and formation of advanced glycation end products (AGEs) that might be a part of unknown regulatory mechanisms. Therefore, here we address the effects of moderate drought on protein dynamics and AGE patterns in pea (Pisum sativum) root nodules. For this, plants inoculated with rhizobial culture were subjected to osmotic stress for one week, harvested, the total protein fraction was isolated from root nodules by phenol extraction, analyzed by bottom-up LC-MS-based proteomics, and AGE patterns were characterized. Surprisingly, despite the clear drought-related changes in phenotype and stomatal conductivity, only minimal accompanying expressional changes (14 rhizobial and 14 pea proteins, mostly involved in central metabolism and nitrogen fixation) could be observed. However, 71 pea and 97 rhizobial proteins (mostly transcription factors, ABC transporters and effector enzymes) were glycated, with carboxymethylation being the major modification type. Thereby, the numbers of glycated sites in nodule proteins dramatically decreased upon stress application. It might indicate an impact of glycation in regulation of transport, protein degradation, central, lipid and nitrogen metabolism. The data are available at Proteome Xchange (accession: PXD024042).

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Medicago truncatula : local response of the root nodule proteome to drought stress

Cited by 3 publications

References 49 publications

Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

Split-root assays for studying legume–rhizobia symbioses, rhizodeposition, and belowground nitrogen transfer in legumes

Protein glycation and drought response of pea (<em>Pisum sativum</em> L.) root nodule proteome: a proteomics approach

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