<i>Medicago truncatula</i>
            as a model organism to study conserved and contrasting aspects of symbiotic and pathogenic signaling pathways

Gavrin, Aleksandr; Schornack, Sebastián

doi:10.1002/9781119409144.ch38

Cited by 2 publications

(2 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To approach these questions, scientists utilized mutant plants in key defense and/or symbiotic genes to decipher the involvement of one signaling pathway in the other. Studies on M. truncatula, or Lotus japonicus, mutants in one or more genes of the common symbiotic signaling pathway (dmi1, dmi2, and dmi3), in NF perception (nfp and lyk3), and in transcription factors (nsp1, nsp2, and nin) corroborate the symbiotic pathway being involved in pathogen interaction beyond the restricted group of symbiotic interactions (Rey et al 2013;Rey et al 2015;Zhang et al 2015;Gavrin and Schornack 2019;Zhang et al 2019;Skiada et al 2020). Other reports challenged this hypothesis and showed no effect of these genes on pathogenic infection and immunity signaling (Huisman et al 2015;Kelly et al 2018).…”

Section: Introductionmentioning

confidence: 91%

Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum tripartite interaction alters nodulation and nitrogen fixation

Batnini

López‐Gómez

Hidalgo-Castellanos

et al. 2023

Preprint

View full text Add to dashboard Cite

Plants encounter a myriad of microorganisms at the root-soil interface that can invade with detrimental or beneficial outcomes. Promoting a symbiotic relationship may interfere with the restriction responses to pathogens. In this study, we established a tripartite Medicago truncatula- Sinorhizobium meliloti-Fusarium oxysporum interaction to study the effect of the interplay between symbiosis and defense on the symbiosis establishment and efficiency in a resistant (A17) and susceptible (TN1.11) genotype to F. oxysporum infection. Our results showed that Sm induced the expression of allene oxide cyclase 1 and 2 (MtAoc1) and (MtAoc2) at 6 and 24 hours post infection (hpi) in A17 and at 24 hpi in TN1.11. This up-regulation of MtAoc1 and MtAoc2 was also observed when co-inoculating TN1.11 genotype with Fo and Sm. Moreover, in this later treatment the expression level of the gene of the common signaling pathway doesn’t make infection 3 (DIM3) was reduced compared to Sm-inoculated plants. This reduction of DIM3 transcripts was concomitant to a decrease in nodule number (NN), nodule fresh weight, and nitrogen-fixing rate (NFR), and to an accumulation of spermine in nodules. This defectiveness in symbiosis parameters, in the co-inoculation treatment, was also observed in A17 genotype, along with an accumulation of phytohormones in leaves and polyamines in nodules. Our results suggest that in the tripartite interaction, the high expression of MtAoc and the decrease of DMI3 transcripts along with the accumulation of phytohormones and polyamines in leaves overlapped with symbiosis establishment and functioning and thus, reduced NN and NFR.

show abstract

Section: Introductionmentioning

confidence: 91%

Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum tripartite interaction alters nodulation and nitrogen fixation

Batnini

López‐Gómez

Hidalgo-Castellanos

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This ‘calcium spiking’ is believed to activate the conserved Calcium/Calmodulin Kinase (CCaMK, also called Does Not Make Infections 3 (DMI3)) and downstream transcription factors (Lévy et al., 2004). CCaMK/DMI3 is a hub for detection of fungal signals and has been co‐opted to play roles in multiple plant–microbe interactions (Genre & Russo, 2016; Cope et al., 2019; Gavrin & Schornack, 2020). The root nodule symbiosis has been a more tractable system for investigation of the CSP, as genetic tools for the glomeromycotinan have yet to be established.…”

Section: Introductionmentioning

confidence: 99%

KIN3 impacts arbuscular mycorrhizal symbiosis and promotes fungal colonisation in Medicago truncatula

et al. 2022

View full text Add to dashboard Cite

Arbuscular mycorrhizal fungi help their host plant in the acquisition of nutrients, and this association is itself impacted by soil nutrient levels. High phosphorus levels inhibit the symbiosis, whereas high nitrogen levels enhance it. The genetic mechanisms regulating the symbiosis in response to soil nutrients are poorly understood. Here, we characterised the symbiotic phenotypes in four Medicago truncatula Tnt1-insertion mutants affected in arbuscular mycorrhizal colonisation. We located their Tnt1 insertions and identified alleles for two genes known to be involved in mycorrhization, RAM1 and KIN3. We compared the effects of the kin3-2 and ram1-4 mutations on gene expression, revealing that the two genes alter the expression of overlapping but not identical gene sets, suggesting that RAM1 acts upstream of KIN3. Additionally, KIN3 appears to be involved in the suppression of plant defences in response to the fungal symbiont. KIN3 is located on the endoplasmic reticulum of arbuscule-containing cortical cells, and kin3-2 mutants plants hosted significantly fewer arbuscules than the wild type. KIN3 plays an essential role in the symbiotic response to soil nitrogen levels, as, contrary to wild-type plants, the kin3-2 mutant did not exhibit increased root colonisation under high nitrogen.

show abstract

Medicago truncatula as a model organism to study conserved and contrasting aspects of symbiotic and pathogenic signaling pathways

Cited by 2 publications

References 152 publications

Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum tripartite interaction alters nodulation and nitrogen fixation

Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum tripartite interaction alters nodulation and nitrogen fixation

KIN3 impacts arbuscular mycorrhizal symbiosis and promotes fungal colonisation in Medicago truncatula

Contact Info

Product

Resources

About