McpandFab-7: molecular analysis of putative boundaries of cis-regulatory domains in the bithorax complex ofDrosophila melanogaster

Karch, François; Galloni, Mireille; Sípos, László; Gausz, János; Gyurkovics, Henrik; Schedl, Paul

doi:10.1093/nar/22.15.3138

Cited by 164 publications

(150 citation statements)

References 61 publications

(76 reference statements)

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…The Mcp element (28) has been shown to harbor a number of regulatory activities, including the ability to act as an insulator (7,19). We have discovered two activities of the Mcp element that are related to the regulation of intergenic transcription.…”

Section: Discussionmentioning

confidence: 99%

“…Genetic, transgenic, and molecular studies have identified a number of classes of distinct cis-regulatory elements within the iab regions capable of directing abdA and AbdB expression. Insulator DNAs have been identified at the boundaries of the iab8-iab7 domains (Frontabdominal-8, Fab8) (4), iab7-iab6 domains (Fab7) (5)(6)(7)(8)(9)(10), and iab5-iab4 domains (Miscadastral pigmentation, Mcp) ( Fig. 1 A) (7,11).…”

Section: E Xpression Of the Abdominal-a (Abda) And Abdominal-b (Abdb)mentioning

confidence: 99%

“…Insulator DNAs have been identified at the boundaries of the iab8-iab7 domains (Frontabdominal-8, Fab8) (4), iab7-iab6 domains (Fab7) (5)(6)(7)(8)(9)(10), and iab5-iab4 domains (Miscadastral pigmentation, Mcp) ( Fig. 1 A) (7,11). Although not all insulators are thought to act in the same way (12,13), it has been suggested that the BX-C insulators organize the different iab regions into discrete chromatin domains ( Fig.…”

Section: E Xpression Of the Abdominal-a (Abda) And Abdominal-b (Abdb)mentioning

confidence: 99%

“…To test this prediction, we examined transcription across the boundaries of the iab5-iab4 and iab6-iab5 regions. The iab5 and iab4 domains are separated by the presumptive Mcp insulator element, which has been defined by chromosomal lesions (28) and molecular analysis (7,11,19) (Fig. 3A).…”

Section: Iab5 Enhancer Selectively Activates a Hox Gene Promotermentioning

confidence: 99%

See 3 more Smart Citations

Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex

Drewell

Bae

Burr

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The extensive infraabdominal (iab) region contains a number of cis-regulatory elements, including enhancers, silencers, and insulators responsible for directing the developmental expression of the abdominal-A and Abdominal-B homeotic genes at the Drosophila bithorax complex. It is unclear how these regulatory elements are primed for activity early in embryogenesis, but the 100-kb intergenic region is subject to a complex transcriptional program. Here, we use molecular and genetic methods to examine the functional activity of the RNAs produced from this region and their role in cis regulation. We show that a subset of these transcripts demonstrates a distinct pattern of cellular localization. Furthermore, the transcripts from each iab region are discrete and the transcripts do not spread across the insulator elements that delineate the iab regions. In embryos carrying a Mcp deletion, the intergenic transcription pattern is disrupted in the iab4 region and the fourth abdominal segment is transformed into the fifth. We propose that intergenic transcription is required early in embryogenesis to initiate the activation of the Drosophila bithorax complex and define the domains of activity for the iab cis-regulatory elements. We also discuss a possible mechanism by which this may occur.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: E Xpression Of the Abdominal-a (Abda) And Abdominal-b (Abdb)mentioning

confidence: 99%

Section: E Xpression Of the Abdominal-a (Abda) And Abdominal-b (Abdb)mentioning

confidence: 99%

Section: Iab5 Enhancer Selectively Activates a Hox Gene Promotermentioning

confidence: 99%

See 2 more Smart Citations

Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex

Drewell

Bae

Burr

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…We show that two different boundary element insertions, one that contains the suppressor of Hairy-wing ½su(Hw) insulator and the other that contains the Mcp element, are able to block the activation of ap by the upstream wing enhancer. The Mcp element from the Drosophila BX-C (Karch et al 1994;Mü ller et al 1999) contains a separable boundary element and a PRE (Busturia et al 2001;Gruzdeva et al 2005), and, like the su(Hw) element, is able to mediate long-range pairing within or even between chromosomes (Sigrist and Pirrotta 1997;Mü ller et al 1999;Vazquez et al 2006). When crossed to an ap promoter deletion, both boundary inserts exhibit the interallelic complementation characteristic of transvection.…”

mentioning

confidence: 99%

Enhancer Blocking and Transvection at the DrosophilaapterousLocus

et al. 2008

View full text Add to dashboard Cite

Intra-and interchromosomal interactions have been implicated in a number of genetic phenomena in diverse organisms, suggesting that the higher-order structural organization of chromosomes in the nucleus can have a profound impact on gene regulation. In Drosophila, homologous chromosomes remain paired in somatic tissues, allowing for trans interactions between genes and regulatory elements on the two homologs. One consequence of homolog pairing is the phenomenon of transvection, in which regulatory elements on one homolog can affect the expression of a gene in trans. We report a new instance of transvection at the Drosophila apterous (ap) locus. Two different insertions of boundary elements in the ap regulatory region were identified. The boundaries are inserted between the ap wing enhancer and the ap promoter and have highly penetrant wing defects typical of mutants in ap. When crossed to an ap promoter deletion, both boundary inserts exhibit the interallelic complementation characteristic of transvection. To confirm that transvection occurs at ap, we generated a deletion of the ap wing enhancer by FRT-mediated recombination. When the wing-enhancer deletion is crossed to the ap promoter deletion, strong transvection is observed. Interestingly, the two boundary elements, which are inserted $10 kb apart, fail to block enhancer action when they are present in trans to one another. We demonstrate that this is unlikely to be due to insulator bypass. The transvection effects described here may provide insight into the role that boundary element pairing plays in enhancer blocking both in cis and in trans.

show abstract

Bithorax Complex

Karch

2002

Encyclopedia of Molecular Biology

View full text Add to dashboard Cite

McpandFab-7: molecular analysis of putative boundaries of cis-regulatory domains in the bithorax complex ofDrosophila melanogaster

Cited by 164 publications

References 61 publications

Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex

Transcription defines the embryonic domains of cis-regulatory activity at the Drosophila bithorax complex

Enhancer Blocking and Transvection at the DrosophilaapterousLocus

Bithorax Complex

Contact Info

Product

Resources

About