2014
DOI: 10.1158/2159-8290.cd-13-0799
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MAX Inactivation in Small Cell Lung Cancer Disrupts MYC–SWI/SNF Programs and Is Synthetic Lethal with BRG1

Abstract: Our knowledge of small cell lung cancer (SCLC) genetics is still very limited, amplifi cation of L-MYC , N-MYC , and C-MYC being some of the well-established gene alterations. Here, we report our discovery of tumor-specifi c inactivation of the MYC-associated factor X gene, MAX , in SCLC. MAX inactivation is mutually exclusive with alterations of MYC and BRG1 , the latter coding for an ATPase of the switch/sucrose nonfermentable (SWI/SNF) complex. We demonstrate that BRG1 regulates the expression of MAX throug… Show more

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Cited by 152 publications
(145 citation statements)
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“…Depletion of BRG1 in AmpMYC/wtBRG1 cells impairs responses to treatments with GC/RA and the combination with azacytidine and SAHA Next we depleted BRG1 in AmpMYC/wtBRG1 cells using two different shBRG1 (shBRG1#1 and shBRG1#4), previously validated by our group [5][6] (Figure 2a; Supplementary Figure S3a). In accordance with our previous results, 5 the depletion of BRG1 markedly reduced the ability of the cells to undergo changes in morphology following treatment with GC/RA and GC/RA combined with A/S (Figure 2b; Supplementary Figure S3b).…”
Section: Resultsmentioning
confidence: 76%
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“…Depletion of BRG1 in AmpMYC/wtBRG1 cells impairs responses to treatments with GC/RA and the combination with azacytidine and SAHA Next we depleted BRG1 in AmpMYC/wtBRG1 cells using two different shBRG1 (shBRG1#1 and shBRG1#4), previously validated by our group [5][6] (Figure 2a; Supplementary Figure S3a). In accordance with our previous results, 5 the depletion of BRG1 markedly reduced the ability of the cells to undergo changes in morphology following treatment with GC/RA and GC/RA combined with A/S (Figure 2b; Supplementary Figure S3b).…”
Section: Resultsmentioning
confidence: 76%
“…The cell lines were authenticated by genotyping for TP53 and other known mutations. 6 Genomic DNA and total RNA were extracted by standard protocols. The study was approved by the relevant institutional review boards and ethics committees.…”
Section: Cell Culturementioning
confidence: 99%
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“…62 ), or by establishing cells stably harboring plasmids expressing cDNAs (MYC: pLOC-MYC; CCND1: pLOC-CCND1; CDC45: pLOC-CCND1; Thermo Scientifi c). In one transient transfection method, cells were seeded in 12-well plates and transfected the next day with 1 μg of plasmid DNA using Lipofectamine 3000 (Invitrogen).…”
Section: Complementation Assaymentioning
confidence: 99%