<i>MAPK8</i> regulates chicken male germ cell differentiation through JNK signaling pathway

Wang, Yingjie; Bi, Yue; Zuo, Qisheng; Zhang, Wenhui; Liu, Dong; He, Na; Cheng, Shaoze; Zhang, Ya‐ni; Li, Bichun

doi:10.1002/jcb.26314

Cited by 7 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, high levels of LncPGCAT‐1 induced JNK phosphorylation. Moreover, JNK signaling was also activated during PGC formation in vivo (Abdellah et al, 2003; Wang, et al, 2017), confirming that LncPGCAT‐1 regulates PGC formation by activating MAPK‐JNK signaling through ILF3.…”

Section: Resultsmentioning

confidence: 90%

P53 and H3K4me2 activate N6‐methylated LncPGCAT‐1 to regulate primordial germ cell formation via MAPK signaling

Zuo

Jin

Zhou

et al. 2020

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) participate in the formation of primordial germ cells (PGCs); however, the identity of the key lncRNAs and the molecular mechanisms responsible for the formation of PGCs remain unknown. Here, we identify a key candidate lncRNA (lncRNA PGC transcript‐1, LncPGCAT‐1) via RNA sequencing of embryonic stem cells, PGCs, and Spermatogonial stem cells (SSCs). Functional experiments confirmed that LncPGCAT‐1 positively regulated the formation of PGCs by elevating the expression of Cvh and C‐kit while downregulating the pluripotency(Nanog) in vitro and in vivo; PAS staining of genital ridges in vivo also showed that interference with LncPGCAT‐1 can significantly reduce the number of PGCs in genital ridges, while overexpression of LncPGCAT‐1 had the opposite result. The result of luciferase reporter assay combined with CHIP‐qPCR showed that the expression of LncPGCAT‐1 was promoted by the transcription factor P53 and high levels of H3K4me2. Mechanistically, the luciferase reporter assay confirmed that mitogen‐activated protein kinase 1 (MAPK1) was the target gene of LncPGCAT‐1 and gga‐mir‐1591. In the ceRNA system, high levels of N6 methylation of LncPGCAT‐1 enhanced the adsorption capacity of LncPGCAT‐1 for gga‐mir‐1591. Adsorption of gga‐mir‐1591 activated the MAPK1/ERK signaling cascade by relieving the gga‐mir‐1591‐dependent inhibition of MAPK1 expression. Moreover, LncPGCAT‐1 interacted with interleukin enhancer binding factor 3 (ILF3) to regulate the ubiquitination of P53 and phosphorylation of JNK. Interaction with ILF3 resulted in positive self‐feedback regulation of LncPGCAT‐1 and activation of JNK signaling, ultimately promoting PGC formation. Altogether, the study expands our knowledge of the function and molecular mechanisms of lncRNAs in PGC development.

show abstract

Section: Resultsmentioning

confidence: 90%

P53 and H3K4me2 activate N6‐methylated LncPGCAT‐1 to regulate primordial germ cell formation via MAPK signaling

Zuo

Jin

Zhou

et al. 2020

Journal Cellular Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…To further clarify the functions of miRNA in various groups, we conducted KEGG pathway enrichment analysis. We found that the miRNA in the Cluster1 is significantly enriched in the SSC formation of related signaling pathways, such as Wnt signaling pathway [ 12 ], JAK-STAT signaling pathway [ 13 ], and MAPK signaling pathway [ 14 ] ( Figure 1(d) ), showing that among the four miRNA clusters, miRNAs in the Cluster1 were more likely to participate in the SSC formation process. Meanwhile, we found these signaling pathways are regulated by miRNA302d target genes.…”

Section: Resultsmentioning

confidence: 99%

miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Zhang

Wang

et al. 2021

Stem Cells International

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are essential factors in the reproductive process of poultry. Here, we found miR-302d is a potential differentiation and negative factor of chicken embryonic stem cells (ESCs) into spermatogonia stem cells (SSCs). The competition mechanism was carried out for the preliminary exploration to determine the relationship among miR-302d, lncRNA-341(interacting with miR-302d), and target gene TLE4. The results showed that lncRNA-341 can competitively bind to miR-302d to decrease the targeted binding of miR-302d and TLE4 which promotes the differentiation of chicken SSCs. Moreover, it is suggested that miR-302d may participate in the Wnt signaling pathway through TLE4.

show abstract

“…Many researches have elucidated the momentous role of MAPK8 in bovine myocyte development, inflammation, and disease treatment. ,, It is worth noting that MAPK8 performs these functions mainly through the JNK pathway. For instance, the study of Wang et al revealed that MAPK8 regulates chicken male germ cytodifferentiation through the JNK signaling pathway . MAP3K11, also known as mixed-spectrum kinase 3 (MLK3), acts as a positive regulator of the c-Jun N-terminal kinase (JNK) signaling pathway .…”

Section: Discussionmentioning

confidence: 99%

“…For instance, the study of Wang et al revealed that MAPK8 regulates chicken male germ cytodifferentiation through the JNK signaling pathway. 54 MAP3K11, also known as mixedspectrum kinase 3 (MLK3), 55 acts as a positive regulator of the c-Jun N-terminal kinase (JNK) signaling pathway. 56 The JNK pathway mainly regulates various physiological processes such as inflammatory response, cytodifferentiation, cell proliferation, and cell apoptosis.…”

Section: ■ Discussionmentioning

confidence: 99%

Circular RNA ACTA1 Acts as a Sponge for miR-199a-5p and miR-433 to Regulate Bovine Myoblast Development through the MAP3K11/MAP2K7/JNK Pathway

Yang

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are a special class of noncoding RNA molecules that regulate many different biological processes. Myogenesis, a complex process, is primarily regulated by myogenic regulatory factors (MRFs) and various noncoding RNAs. However, the functions and regulatory mechanisms of circRNAs in myoblast development are unclear. In this study, we analyzed circRNA sequencing data of bovine myocyte tissues and identified circACTA1. Functional assays showed that circACTA1 could inhibit bovine myocyte proliferation and promote cell apoptosis and cytodifferentiation. In addition, circACTA1 could promote muscle repair in vivo. Mechanistically, luciferase assay and RNA immunoprecipitation were used to examine the interaction between circACTA1, miR-199a-5p, miR-433, and the target genes MAP3K11 and MAPK8. Meanwhile, we found that miR-199a-5p and miR-433 could suppress the expression of MAP3K11 and MAPK8, respectively. However, circACTA1 could mitigate this effect and activate the JNK signaling pathway. In conclusion, our results suggest that circACTA1 regulates the multiplication, apoptosis, and cytodifferentiation of bovine myocytes by competitively combining with miR-199a-5p and miR-433 to activate the mitogenactivated protein kinase kinase kinase 11 (MAP3K11)/mitogen-activated protein kinase kinase 7 (MAP2K7)/JNK signaling pathway.

show abstract

MAPK8 regulates chicken male germ cell differentiation through JNK signaling pathway

Cited by 7 publications

References 21 publications

P53 and H3K4me2 activate N6‐methylated LncPGCAT‐1 to regulate primordial germ cell formation via MAPK signaling

P53 and H3K4me2 activate N6‐methylated LncPGCAT‐1 to regulate primordial germ cell formation via MAPK signaling

miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation

Circular RNA ACTA1 Acts as a Sponge for miR-199a-5p and miR-433 to Regulate Bovine Myoblast Development through the MAP3K11/MAP2K7/JNK Pathway

Contact Info

Product

Resources

About