<i>Magnaporthe oryzae</i> Induces the Expression of a MicroRNA to Suppress the Immune Response in Rice

Zhang, Xin; Bao, Yalin; Shan, Deqi; Wang, Zhihui; Song, Xiujun; Wang, Zhaoyun; Wang, Jiansheng; He, Ling; Wu, Liang; Zhang, Zhengguang; Niu, Dongdong; Jin, Hailing; Zhao, Hongwei

doi:10.1104/pp.17.01665

Cited by 112 publications

(100 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These reports suggest that the miRNAs indirectly participate in BPH stress responses through altering the metabolic processes and hormone regulation. Interestingly, miR319 negatively regulated immunity to rice ragged stunt virus and blast fungus by repressing the expression of OsTCP21, leading to decrease JA-mediated defenses [27,28], whilst members of the miR319 family were upregulated in the BPH6G plants ( Fig. 3b), suggesting that miR319 might separately regulate rice immunity against BPH and pathogens.…”

Section: Discussionmentioning

confidence: 99%

“…2a), many of which belonged to known miRNA families including miR156, miRNA160, miR166, miR169, miR1846, miR1861 and miR319 (Additional file 3: Table S2). Members of the miRNA families were reported to be involved not only in growth, development, grain size and hormone signaling, but also in response to biotic and abiotic stress [21][22][23][24][25][26][27][28][29][30][31]. These BPH6 responsive DEMs might be involved in response to BPH.…”

Section: Identification Of Mirnas Related To Bph Resistancementioning

confidence: 99%

“…miRNAs specifically regulate target gene expression through binding complementary sequences to degrade mRNA or inhibit translation [19]. Plant miRNAs are involved in many development processes, including hormone signal transduction, and leaf, floral, shoot, root and vascular development [20][21][22], and play significant roles in abiotic and biotic stress responses [23][24][25][26][27][28]. miR160 is associated with local defense and systemic acquired resistance to potato late blight [24].…”

Section: Introductionmentioning

confidence: 99%

“…miR160 is associated with local defense and systemic acquired resistance to potato late blight [24]. miR166, miR169 and miR319 participate in the regulation of rice immunity against the blast fungus Magnaporthe oryzae [25][26][27][28]. However, few miRNAs have been revealed functioning in insect response.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

Tan

Guo

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: The brown planthopper (BPH, Nilaparvata lugens Stål) is a kind of phloem-feeding pest that adversely affects rice yield. Recently, the BPH-resistance gene, BPH6, was cloned and applied in rice breeding to effectively control BPH. However, the molecular mechanisms underlying BPH6 are poorly understood.Results: Here, an integrated miRNA and mRNA expression profiling analysis was performed on BPH6-transgenic (BPH6G) and Nipponbare (wild type, WT) plants after BPH infestation, and a total of 217 differentially expressed miRNAs (DEMs) and 7874 differentially expressed mRNAs (DEGs) were identified. 29 miRNAs, including members of miR160, miR166 and miR169 family were opposite expressed during early or late feeding stages between the two varieties, whilst 9 miRNAs were specifically expressed in BPH6G plants, suggesting involvement of these miRNAs in BPH6-mediated resistance to BPH. In the transcriptome analysis, 949 DEGs were opposite expressed during early or late feeding stages of the two genotypes, which were enriched in metabolic processes, cellular development, cell wall organization, cellular component movement and hormone transport, and certain primary and secondary metabolite synthesis. 24 genes were further selected as candidates for BPH resistance. Integrated analysis of the DEMs and DEGs showed that 34 miRNAs corresponding to 42 target genes were candidate miRNA-mRNA pairs for BPH resistance, 18 pairs were verified by qRT-PCR, and two pairs were confirmed by in vivo analysis. Conclusions:For the first time, we reported integrated small RNA and transcriptome sequencing to illustrate resistance mechanisms against BPH in rice. Our results provide a valuable resource to ascertain changes in BPHinduced miRNA and mRNA expression profiles and enable to comprehend plant-insect interactions and find a way for efficient insect control.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Identification Of Mirnas Related To Bph Resistancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A combined microRNA and transcriptome analyses illuminates the resistance response of rice against brown planthopper

Tan

Guo

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…One example is that M. oryzae specifically induced the expression of rice miR319, whose target gene encodes a transcription factor OsTCP21. OsTCP21 is a positive regulator of the rice defense response against the blast disease, likely via inducing JA synthesis genes LOX2 and LOX5 (Zhang et al 2018). Therefore, M. oryzae is able to reduce rice JA level by suppressing JA synthesis via inducing rice miR319.…”

Section: Jas In M Oryzae-rice Pathosystemmentioning

confidence: 99%

Phytohormones: the chemical language in Magnaporthe oryzae-rice pathosystem

2018

View full text Add to dashboard Cite

Phytohormones (also named as plant hormones) are chemicals produced by plants in order to modulate various aspects of plant development, stress responses and defence. Recent studies revealed that fungi can also produce phytohormones or phytohormone-mimiking molecules, while it remains poorly understood about the details in the role and regulatory mechanism of such fungal produced phytohormonal molecules in plant-fungus interactions. The rice-blast fungus Magnaporthe oryzae imposes a great threat to global food security. Intensive investigation has been conducted to elucidate M. oryzae pathogenicity and rice (Oryza sativa L.) defense mechanism against blast disease, in order to provide theoretical basis and/or identify potential target(s) for developing novel disease control strategies, as well as for breeding of resistance varieties. Phytohormones have been demonstrated to play conserved and divergent roles in fine-tuning the balance of rice growth and immunity towards M. oryzae. Meanwhile, M. oryzae evolved elaborate strategy to manipulate the rice phytohormones metabolism, or even directly produce and secrete phytohormones, during their invasion process. In this review, we discuss the chemical communication in term of phytohormones in M. oryzae-rice pathosystem.

show abstract