<i>Lycium barbarum</i> polysaccharide protects rats and cardiomyocytes against ischemia/reperfusion injury via Nrf2 activation through autophagy inhibition

Pān, Hào; Niu, Lin; Wu, Yihao; Chen, Liuying; Zhou, Xiaowei; Zhao, Yan

doi:10.3892/mmr.2021.12418

Cited by 19 publications

(13 citation statements)

References 66 publications

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“…In the heart, dysregulated autophagy induces death of cardiomyocytes and actively mediates cardiac injury and dysfunction in some conditions, including reperfusion injury, doxorubicin cardiomyopathy, and lysosomal storage disorders [ 32 ]. Existing investigation has documented the efficacy of polysaccharide, Lycium barbarum polysaccharide to be exact, on inhibiting autophagy so as to protect the cardiomyocytes [ 33 ]. This discovery made us wondered if TSLFACP could inhibit autophagy.…”

Section: Discussionmentioning

confidence: 99%

Cardioprotective effect of crude polysaccharide fermented by Trametes Sanguinea Lyoyd on doxorubicin-induced myocardial injury mice

Shen

Yang

Huang

et al. 2023

BMC Pharmacol Toxicol

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Doxorubicin (DOX) is a broad-spectrum anti-tumor drug, but its clinical application is greatly limited because of the cardiotoxicity. Thus, exploration of effective therapies against DOX-induced cardiotoxicity is necessary. The aim of this study is to investigate the effects and possible mechanisms of Trametes Sanguinea Lyoyd fermented crude polysaccharide (TSLFACP) against DOX-induced cardiotoxicity. We investigated the protective effects of TSLFACP on myocardial injury and its possible mechanisms using two in vitro cells of DOX-treated cardiomyocytes H9C2 and embryonic myocardial cell line CCC-HEH-2 and a in vivo mouse model of DOX-induced myocardial injury. We found that TSLFACP could reverse DOX-induced toxicity in H9C2 and CCC-HEH-2 cells. Similarly, we found that when pretreatment with TSLFACP (200 mg/kg, i.g.) daily for 6 days, DOX-induced myocardial damage was attenuated, including the decrease in serum myocardial injury index, and the amelioration in cardiac histopathological morphology. Additionally, immunohistochemistry and western blotting were used to identify the underlying and possible signal pathways. We found that TSLFACP attenuated the expression of LC3-II, Beclin-1 and PRAP induced by DOX. In conclusion, our results demonstrated that TSLFACP could protect against DOX-induced cardiotoxicity by inhibiting autophagy and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Cardioprotective effect of crude polysaccharide fermented by Trametes Sanguinea Lyoyd on doxorubicin-induced myocardial injury mice

Shen

Yang

Huang

et al. 2023

BMC Pharmacol Toxicol

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“…Moreover, the cardioprotective effects of Salvianolic acid B on acute myocardial infarction in rats were also found to be associated with autophagy promotion and the activation of Nrf2-mediated redox signaling documented by increased levels of superoxide dismutase [ 160 ]. The protective effects of Lycium barbarum polysaccharide against ischemia/reperfusion injury in rats and cardiomyocytes via Nrf2 activation through autophagy inhibition were also presented [ 161 ]. The interplay between redox signaling and autophagy in ischemic heart disease is supported also by findings that resveratrol provides cardioprotection against I/R injury via redox signaling activation and autophagy induction [ 162 ].…”

Section: Interplay Between Autophagy and Redox Signaling In Cardiovas...mentioning

confidence: 99%

“…An improvement in cardiac performance and redox homeostasis by CAR and CAA included the augmenting of anti-oxidative enzymes expression and activities. Further therapeutic approaches based on the modulation of both the Nrf2 signaling pathway and autophagy are presented [ 161 , 165 , 166 ]. A model of doxorubicin-induced cardiotoxicity in mice presented data showing the cardioprotective potential of a natural quinone β-LAPachone via a modulation of both the AMPK-Nrf2 and AMPK-mTOR signaling pathways [ 165 ].…”

Section: Interplay Between Autophagy and Redox Signaling In Cardiovas...mentioning

confidence: 99%

The Interplay between Autophagy and Redox Signaling in Cardiovascular Diseases

Boťanská

Dovinová

Barančı́k

2022

Cells

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Reactive oxygen and nitrogen species produced at low levels under normal cellular metabolism act as important signal molecules. However, at increased production, they cause damage associated with oxidative stress, which can lead to the development of many diseases, such as cardiovascular, metabolic, neurodegenerative, diabetes, and cancer. The defense systems used to maintain normal redox homeostasis plays an important role in cellular responses to oxidative stress. The key players here are Nrf2-regulated redox signaling and autophagy. A tight interface has been described between these two processes under stress conditions and their role in oxidative stress-induced diseases progression. In this review, we focus on the role of Nrf2 as a key player in redox regulation in cell response to oxidative stress. We also summarize the current knowledge about the autophagy regulation and the role of redox signaling in this process. In line with the focus of our review, we describe in more detail information about the interplay between Nrf2 and autophagy pathways in myocardium and the role of these processes in cardiovascular disease development.

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“…These factors can be used as indicators of mPTP opening. Another study showed that LBP can alleviate MI by regulating Nrf2-related autophagy ( 27 ). In addition, LBP clearly decreases the positivity rate of myocardial Bax protein and myocardial cell apoptosis and increases the positivity rate of Bcl-2 in a dose-dependent manner ( 28 ).…”

Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharide protects cardiomyocytes from hypoxia/reoxygenation injury via activation of SIRT3/CypD signaling

Wu¹,

Liu²,

Yu³

et al. 2023

Ann Transl Med

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Background Myocardial ischemia-reperfusion is a common pathological feature of many heart and vascular diseases, but the molecular mechanism of this process is still unclear, and there is no effective way to protect cardiomyocytes. The aim of this study was to examine the effects and underlying molecular mechanisms of Lycium barbarum polysaccharide (LBP) on myocardial ischemia-reperfusion injury in cardiomyocytes. Methods The cardiomyocyte cell line H9c2 were used to establish an in vitro hypoxia/reoxygenation (H/R) model. After treatment with LBP and/or the SIRT3 inhibitor 3-TYP, cell morphology was observed under the light microscopy. The Cell Counting Kit (CCK)-8 and 5-ethynyl-2'-deoxyuridine (EdU) assay were used to detect cell proliferation, and flow cytometry was performed to assess cell apoptosis. The lysine (166)-acetylation of CypD1 was determined by co-immunoprecipitation assay. Enzyme-linked immunosorbent assay (ELISA) was used to determine the lactate dehydrogenase (LDH) level in the culture medium. Na + -K + -ATPase activity, Ca 2+ -ATPase activity, and nitric oxide (NO) levels were measured. Results LBP alleviated cell damage and upregulated STIR3 expression in a dose-dependent manner. Upregulated SIRT3 expression and suppressed acetylation of CypD were also observed in H/R-induced H9c2 cells treated with LBP. Indeed, LBP remarkably reversed the inhibition of proliferation and cell apoptosis in H/R-induced H9c2 cells by activating SIRT3/CypD signaling. Blockade of SIRT3 with SIRT3 inhibitor (3-TYP) inhibited the protective effect of LBP on H9c2 cells. LBP markedly alleviated the H/R-induced increase of LDH release, and the decrease of Na + -K + -ATPase activity, Ca 2+ -ATPase activity, and NO levels. Inhibition of SIRT3 restored the protective effects of LBP. Conclusions LPB induced deacetylation of CypD by upregulating SIRT3, thereby protecting mitochondrial function and relieving H/R-induced injury in cardiomyocytes.

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Lycium barbarum polysaccharide protects rats and cardiomyocytes against ischemia/reperfusion injury via Nrf2 activation through autophagy inhibition

Abstract: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.

Cited by 19 publications

References 66 publications

Cardioprotective effect of crude polysaccharide fermented by Trametes Sanguinea Lyoyd on doxorubicin-induced myocardial injury mice

Cardioprotective effect of crude polysaccharide fermented by Trametes Sanguinea Lyoyd on doxorubicin-induced myocardial injury mice

The Interplay between Autophagy and Redox Signaling in Cardiovascular Diseases

Lycium barbarum polysaccharide protects cardiomyocytes from hypoxia/reoxygenation injury via activation of SIRT3/CypD signaling

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