Lotus corniculatus Regulates the Inflammation Induced by Bradykinin in a Murine Model of Pleurisy

Abstract

This study evaluated the anti-inflammatory efficacy of the crude extract (CE), the fractions derived from hexane (HEX), ethyl acetate (AcOEt), n-butanol (BuOH), and aqueous (Aq) and isolated compounds (oleanolic acid or kaempferitrin) obtained from the aerial parts of Lotus corniculatus var. São Gabriel in mice with bradykinin-induced pleurisy. Swiss mice were used for the In Vivo experiments. Inflammatory parameters [leukocytes; exudate concentrations; myeloperoxidase and adenosine-deaminase activities, and nitric oxide and interleukin-17 levels] were evaluated 4 h after pleurisy induction. The crude extract of Lotus corniculatus, its derived fractions, and isolated compounds inhibited leukocytes and the exudate. This inhibitory effect was associated with decreased of myeloperoxidase and adenosine-deaminase activities, nitric oxide products, and IL-17A levels. Lotus corniculatus presented important anti-inflammatory action by inhibiting leukocyte influx and exudate concentrations. This effect was directly related to the inhibition of nitric oxide and interleukinin17 levels. Oleanolic acid and kaempferitrin can account for these anti-inflammatory effects.

Kaempferitrin inhibits proliferation, induces apoptosis, and ameliorates inflammation in human rheumatoid arthritis fibroblast‐like synoviocytes

“…These findings suggest that kaempferitrin exerts immunostimulatory effects on immune responses that are mediated by splenocytes, macrophages, peripheral blood mononuclear cells, and natural killer cells (Del Carmen Juarez‐Vazquez et al, ). In addition, kaempferitrin has been shown to exert anti‐inflammatory activity by inhibiting leukocyte influx and exudate concentrations in a murine model of pleurisy, which is related to the inhibition of nitric oxide and IL‐17 levels (Pereira et al, ). Hamzah et al () reported that kaempferitrin exerted prominent cytotoxic effect on the cervical cancer cell line HeLa.…”

Biologically Active Triterpenoids and Their Cardioprotective and Anti- Inflammatory Effects

“…One of the several types of the triterpenoid compounds found in the peel is oleanolic acid (OA) [50]. In the fruits of the sultana vines and several American Vitis species, OA was the main constituent of the chloroform extracts of cuticular waxes of the fruits [8].…”

Clove Extract Inhibits Tumor Growth and Promotes Cell Cycle Arrest and Apoptosis

“…This compound was then subjected to NMR analysis: 1 H-NMR (500 MHz, CDCl 3 ): δ5.27 (1H, m, H-12), 3.25 (1H, dd, J = 15.0, 5.9 Hz), 2.81 (1H, dd, J = 12.3, 5.5 Hz), 1.13 (3H, s), 1.06 (3H, s), 0.98 (3H, s), 0.92 (3H, s), 0.90 (3H, s), 0.77 (3H, s), 0.74 (3H, s); 13 C-NMR (125 MHz, CDCl 3 ): δ38.4 (C-1), 27.1 (C-2), 79.0 (C-3), 38.7 (C-4), 55.2 (C-5), 18.3 (C-6), 32.9 (C-7), 39.3 (C-8), 47.6 (C-9), 37.1 (C-10), 22.9 (C-11), 122.6 (C-12), 143.6 (C-13), 41.6 (C-14), 27.7 (C-15), 23.5 (C-16), 46.5 (C-17), 40.9 (C-18), 45.9 (C-19), 30.6 (C-20), 33.8 (C-21), 32.4 (C-22), 28.1 (C-23), 15.3 (C-24), 15.5 (C-25), 17.1 (C-26), 25.9 (C-27), 183.4 (C-28), 33.4 (C-29), 23.4 (C-30). Based on comparisons with published data (13), the purified compound was identified as (3β)-3-hydroxyolean-12-en-28-oic acid (oleanolic acid, OA). The cytotoxic activity of OA was analyzed in various human cancer cell lines.…”