<i>Litsea cubeba</i>
 leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity

Nguyen, Hai; Meile, Jean-Christophe; Lebrun, Marc; Caruso, Domenico; Chu-Ky, Son; Sarter, Samira

doi:10.1111/lam.12837

Cited by 38 publications

(36 citation statements)

References 18 publications

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“…Here, we examined the antimicrobial activity of the total EO constituents to explore potential synergistic effects. Nevertheless, in concordance with a previous study [9,19], the L. cubeba EO showed low MICs (≥ 700 µg/mL) against the tested microbes (Table 3) [17].…”

Section: Antimicrobial Activity Of the Crude Ethyl Acetate Extract (Csupporting

confidence: 91%

“…This combination showed broad-spectrum inhibitory effects against various microbial species tested. In fact, citral, carveol, and limonene are phenolic substances with polarity characteristics that might act on the bacterial cell membrane, inactivate cell adhesions, and/or interact with the outer and inner membrane proteins [19,29,33,34]. Consequently, the EO treatment might result in the weakening of cell structure and inhibition of cell growth by creating holes on the cell wall and increasing the cell permeability [29].…”

Section: Discussionmentioning

confidence: 99%

“…In order to investigate the activity of the L. cubeba EO against pathogenic bacteria, the time-kill curve assay was performed using E. coli ATCC 25922 as a model [19]. The experiments were designed as follows: tubes including bacterial suspension (10 8 CFU/mL) were exposed to four different EO concentrations (1, 2, 5, and 10 MIC) and control tube (bacterial culture without EO).…”

Section: Time-killing Assaymentioning

confidence: 99%

“…The effect of the L. cubeba fruit EO on cell viability was evaluated using the LIVE/DEAD BacLight viability kit (Invitrogen TM , Molecular Probes Inc., OR, USA) [19]. Briefly, the E. coli cultures were prepared in MHB as described above, then each tube containing 5 mL of cell suspension was incubated with 1 MIC and 0.5 MIC of the EO at 37 • C. After 2 h of exposure, the cells were harvested by centrifugation at 10,000x g for 10 min, then the supernatant was removed and pellets were re-suspended in NaCl 0.85%.…”

Section: Effects Of L Cubeba Eo On Bacterial Cell Viability and Morpmentioning

confidence: 99%

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Characterization of Endophytic Streptomyces griseorubens MPT42 and Assessment of Antimicrobial Synergistic Interactions of Its Extract and Essential Oil from Host Plant Litsea cubeba

Nguyen

et al. 2019

Antibiotics

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The present study aimed to evaluate the synergistic effects of the crude ethyl acetate extract (CEAE) from endophytic actinomycete MPT42 and essential oil (EO) of the same host plant Litsea cubeba. The isolate MPT42, exhibiting broad-spectrum antimicrobial activities and harboring all three antibiotic-related biosynthetic genes pks-I, pks-II, and nrps, was identified as Streptomycete griseorubens based on an analysis of the morphology, physiology, and 16S rDNA sequence. Minimum inhibitory concentrations (MICs) and the fractional inhibitory concentration index were used to estimate the synergistic effects of various combined ratios between CEAE or antibiotics (erythromycin, vancomycin) and EO toward 13 microbial strains including pathogens. L. cubeba fruit EO, showing the main chemical constituents of 36.0% citral, 29.6% carveol, and 20.5% limonene, revealed an active-low against tested microbes (MICs ≥ 600 μg/mL). The CEAE of S. griseorubens culture exhibited moderate–strong antimicrobial activities against microbes (MICs = 80–600 μg/mL). Analysis of the mechanism of action of EO on Escherichia coli ATCC 25922 found that bacterial cells were dead after 7 h of the EO treatment at 1 MIC (5.5 mg/mL), where 62% cells were permeabilized after 2 h and 3% of them were filament (length ≥ 6 μm). Combinations of CEAE, erythromycin, or vancomycin with EO led to significant synergistic antimicrobial effects against microbes with 4–16 fold reduction in MIC values when compared to their single use. Interestingly, the vancomycin–EO combinations exhibited a strong synergistic effect against five Gram-negative bacterial species. This could assume that the synergy was possibly due to increasing the cell membrane permeability by the EO acting on the bacterial cells, which allows the uptake and diffusion of antimicrobial substances inside the cell easily. These findings in the present study therefore propose a possible alternative to combat the emergence of multidrug-resistant microbes in veterinary and clinics.

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Section: Antimicrobial Activity Of the Crude Ethyl Acetate Extract (Csupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Time-killing Assaymentioning

confidence: 99%

Section: Effects Of L Cubeba Eo On Bacterial Cell Viability and Morpmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Endophytic Streptomyces griseorubens MPT42 and Assessment of Antimicrobial Synergistic Interactions of Its Extract and Essential Oil from Host Plant Litsea cubeba

Nguyen

et al. 2019

Antibiotics

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“…The second investigated species from the Lauraceae family ( Litsea cubeba Li.cu EO) showed geranial (36.4 %), neral (32.5 %) and limonene (16.3 %) as the main compounds. Our results were different from the same species collected in Vietnam where 1,8‐cineole and sabinene were presented in the highest percentage …”

Section: Resultsmentioning

confidence: 41%

Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines

Najar

Shortrede

Pistelli

et al. 2019

Chemistry & Biodiversity

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The in vitro cytotoxic activity on human cancer cell lines of sixteen commercial EOs such as Aloysia citriodora, Boswellia sacra, Boswellia serrata, Cinnamomum zeylanicum, Cistus ladanifer, Citrus × aurantium, Citrus limon, Citrus sinensis, Cymbopogon citratus, Foeniculum vulgare, Illicium verum, Litsea cubeba, Satureja montana, Syzygium aromaticum, Thymus capitatus and Thymus vulgaris was performed using the MTT reduction assay. The screening was carried out on human cancer cells of breast adenocarcinoma (MCF7, T47D and MDA-MB-231), chronic myelogenous erythroleukemia (K562) and neuroblastoma cell lines (SH-SY5Y). C. zeylanicum and L. cubeba EOs were the most active on almost all the cell lines studied and thus could be promising as an anticancer agent. These two species showed a difference in their composition even though they belong to the Lauraceae family. Almost 57 % of the true cinnamon composition was made of (E)-cinnamaldehyde, while L. cubeba showed citral as the major compound (68.9 %). The K562 cells were the most sensitive to these oils with an IC 50 ranging from 5.2 parts-per million (ppm) (C. zeylanicum) to 11.1 ppm (L. cubeba). The latter oil also showed an important cytotoxicity on MDA-MB-231 (13.4 ppm).

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Lactobacillus acidophilus and Anethum graveolens essential oil in Iranian cheese against Escherichia coli O157:H7

Langroodi

Mehdizadeh

Majidi

et al. 2020

Flavour & Fragrance J

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The impacts of Anethum graveolens essential oil (DEO) alone and its incorporation with Lactobacillus acidophilus on the viability of Escherichia coli O157 in cheese were determined during keeping time. The impact of various levels of DEO and L acidophilus, on pH, organoleptic properties and E coli O157 number in a produced cheese was evaluated. Different levels of the DEO and its incorporation with L acidophilus detectably declined the E coli O157 numbers, in comparison with the control sample. In addition, on pH reduction, the count of E coli O157 also declined in cheese treatments. The increasing impacts on the viability of E coli O157 were reported in various samples of DEO and L acidophilus. Also, the combination of DEO and L acidophilus in treatments concluded detectably enhanced organoleptic characteristics. The detectable synergistic inhibitory impact of probiotic bacteria and DEO on E coli O157 was concluded in the current research, which confirms DEO‐positive activity in dairy production.

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Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity

Cited by 38 publications

References 18 publications

Characterization of Endophytic Streptomyces griseorubens MPT42 and Assessment of Antimicrobial Synergistic Interactions of Its Extract and Essential Oil from Host Plant Litsea cubeba

Characterization of Endophytic Streptomyces griseorubens MPT42 and Assessment of Antimicrobial Synergistic Interactions of Its Extract and Essential Oil from Host Plant Litsea cubeba

Chemical Composition and in Vitro Cytotoxic Screening of Sixteen Commercial Essential Oils on Five Cancer Cell Lines

Lactobacillus acidophilus and Anethum graveolens essential oil in Iranian cheese against Escherichia coli O157:H7

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