LINC01133 aggravates the progression of hepatocellular carcinoma by activating the PI3K/AKT pathway

Abstract

LncRNAs exhibit crucial roles in various pathological diseases, including hepatocellular carcinoma (HCC). Therefore, it is significant to recognize the dysregulated lncRNAs in HCC progression. Recently, LINC01133 has been identified in several tumors. However, the biological role of LINC01133 in HCC remains poorly understood. Currently, we focused on the function of LINC01133 in HCC development. We observed that LINC01133 was significantly increased in HCC cells including HepG2, Hep3B, MHCC-97L, SK-Hep-1, and MHCC-97H cells compared with the normal human liver cell line HL-7702. In addition, PI3K/AKT signaling was highly activated in HCC cells. Knockdown of LINC01133 was able to inhibit HCC cell proliferation, cell colony formation, cell apoptosis, and blocked cell cycle arrest in the G1 phase. For another, downregulation of LINC01133 repressed HCC cell migration and invasion. Subsequently, the PI3K/AKT signaling pathway was strongly suppressed by silence of LINC01133 in Hep3B and HepG2 cells. Then, in vivo tumor xenografts models were established using Hep3B cells to explore the function of LINC01133 in HCC progression. Consistently, our study indicated that knockdown of LINC01133 dramatically repressed HCC tumor progression through targeting the PI3K/AKT pathway in vivo. Taken these together, we revealed that LINC01133 contributed to HCC progression by activating the PI3K/AKT pathway. K E Y W O R D S hepatocellular carcinoma (HCC), LINC01133, PI3K/AKT signaling pathway J Cell Biochem. 2019;120:4172-4179. wileyonlinelibrary.com/journal/jcb 4172 |

MiR-216a-5p inhibits tumorigenesis in Pancreatic Cancer by targeting TPT1/mTORC1 and is mediated by LINC01133

“…In previous studies, C/EBPβ-LINC01133 axis was found to promote proliferation of pancreatic ductal adenocarcinoma cells by upregulating CCNG1 [24]. In addition, LINC01133 was found to aggravate the progression of hepatocellular carcinoma and non-small cell lung cancer by activating the PI3K/ATK pathway or by repressing KLF2/E-cadherin [25,26]. Conversely, LINC01133 was reported to act as a tumor suppressor in the context of gastric cancer, which was downregulated in tumor cells and acts as a ceRNA by sponging miR-106a-3p to regulate APC expression and Wnt/β-catenin pathway [27,28].…”

Gene signatures and prognostic values of m1A-related regulatory genes in hepatocellular carcinoma

“…In researching the possible carcinogenic mechanism of m1A-related regulatory genes in HCC, PI3K/Akt signaling pathway was found to be the most important item through KEGG analysis. The PI3K/Akt signaling pathway has been reported to involve in proliferation and anti-apoptosis process in HCC 40,41 , which pointed out a theoretical basis for further mechanism research on m1A-related regulatory genes. Following, relevant validations via cell and animal experiment are crucial to carry out to reveal how m1A-related regulatory genes regulate PI3K/Akt pathway in HCC.…”

ADH7, miR‑3065 and LINC01133 are associated with cervical cancer progression in different age groups

“…Numerous studies have indicated that the lncRNA LINC01133 serves as a regulator of various cancer types; for example, LINC01133 was revealed to suppress the invasiveness, migration and proliferation of nasopharyngeal carcinoma cells, via interaction with Y box binding protein 1 (48). Furthermore, Zheng et al (49) demonstrated that LINC01133 contributes to the progression of HCC via activation of the PI3K/AKT pathway. It has also been identified that LINC01133 was upregulated in lung squamous cell cancer cells and was associated with poorer overall survival outcomes (50).…”