<i>Leptospira interrogans</i> serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Grippi, Francesca; Giudice, Elisabetta; Pietro, Simona Di; Sciacca, Carmela; Santangelo, Francesco; Galluzzo, Paola; Barreca, Santino; Guercio, Annalisa

doi:10.2478/jvetres-2020-0021

Cited by 13 publications

(29 citation statements)

References 17 publications

(23 reference statements)

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“…Studies from Europe and Asia also show a high ST diversity among different genera of small mammals [ 72 , 73 , 74 , 75 ]. Serogroups detected in our study were detected worldwide in different host species, e.g., serogroup Australis in swine, goats, cats and dogs [ 76 , 77 , 78 , 79 ]; serogroup Grippotyphosa in cattle, sheep, wild boars, dogs and horses [ 16 , 80 , 81 , 82 , 83 ]; and serogroup Sejroe in cattle [ 84 , 85 ].…”

Section: Discussionmentioning

confidence: 97%

Influence of Season, Population and Individual Characteristics on the Prevalence of Leptospira spp. in Bank Voles in North-West Germany

Schmidt

Obiegala

Imholt

et al. 2021

Biology

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Leptospirosis is a worldwide zoonotic disease with more than 1 million human cases annually. Infections are associated with direct contact to infected animals or indirect contact to contaminated water or soil. As not much is known about the prevalence and host specificity of Leptospira spp. in bank voles (Clethrionomys glareolus), our study aimed to evaluate Leptospira spp. prevalence and genomospecies distribution as well as the influence of season, host abundance and individual characteristics on the Leptospira prevalence. Bank voles, which are abundant and widely distributed in forest habitats, were collected in the years 2018 to 2020 in North-West Germany, covering parts of North Rhine-Westphalia and Lower Saxony. The DNA of 1817 kidney samples was analyzed by real-time PCR targeting the lipl32 gene. Positive samples were further analyzed by targeting the secY gene to determine Leptospira genomospecies and multilocus sequence typing (MLST) to determine the sequence type (ST). The overall prevalence was 7.5% (95% confidence interval: 6.4–8.9). Leptospira interrogans (83.3%), L. kirschneri (11.5%) and L. borgpetersenii (5.2%) were detected in bank voles. Increasing body weight as a proxy for age increased the individual infection probability. Only in years with high bank vole abundance was this probability significantly higher in males than in females. Even if case numbers of human leptospirosis in Germany are low, our study shows that pathogenic Leptospira spp. are present and thus a persisting potential source for human infection.

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Section: Discussionmentioning

confidence: 97%

Influence of Season, Population and Individual Characteristics on the Prevalence of Leptospira spp. in Bank Voles in North-West Germany

Schmidt

Obiegala

Imholt

et al. 2021

Biology

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“…The gold standard microscopic agglutination test (MAT) was carried out on all the serum samples according to the OIE Manual of Terrestrial Animals [2,36].…”

Section: Serological Test For Leptospirosismentioning

confidence: 99%

“…Leptospirosis is a zoonosis with a worldwide distribution [1,2]. It is caused by pathogenic helical spirochetes of the Leptospira genus (family Leptospiraceae, order Spirochaetales).…”

Section: Introductionmentioning

confidence: 99%

Leptospira interrogans Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System

Grippi

Blanda

Presti

et al. 2022

Veterinary Sciences

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Bovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of Leptospira interrogans serogroup Pomona that occurred in cattle farms within the Nebrodi Park and was mainly characterized by full-term abortion. Blood and urine samples were collected at different time points from animals of six different farms (Farms A–F) sharing the same grazing area. Research of antibodies against pathogenic Leptospira species in serum samples was carried out via Micro Agglutination Test (MAT). Urine samples were subjected to pathogen isolation and molecular analyses via TaqMan Real Time-PCR. Genotyping of Leptospira species was obtained by Multi-locus sequence typing. MAT detected antibodies against Leptospira interrogans serogroup Pomona in serum samples of all the farms. Pathogenic Leptospira spp. DNA and culture isolation was obtained from urine samples. Genotyping confirmed the excretion of L. interrogans serogroup Pomona. This study describes clinical manifestations, diagnostic implications and epidemiological characteristics of an outbreak in cattle due to L. interrogans Pomona in a protected multi-host area, where domestic and wild animals share the same habitat, suggesting a role of wild species in transmission and persistence of Pomona serogroup among cattle.

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“…Microscopic Agglutination Test (MAT) OIE guidelines were followed to perform the MAT [18,27], a serological test detecting antibodies to specific serovars using live leptospiral antigens. The strains, provided by the Italian National Reference Centre for Leptospirosis, were grown in liquid Leptospira Ellinghausen-McCullogh-Johnson-Harris (EMJH) culture medium for 4-8 days at 30 For DNA extraction from kidney, the surface was flamed and 1 g of tissue withdrawn and homogenized in 9 mL of sterile physiological solution with Stomacher ® 80 Biomaster (Seward Limited, London, UK).…”

Section: Serological Testmentioning

confidence: 99%

Leptospira in Slaughtered Fattening Pigs in Southern Italy: Serological Survey and Molecular Typing

Macaluso

Torina

Blanda

et al. 2022

Animals

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Leptospirosis is a re-emerging zoonosis of worldwide significance; a wide spectrum of wild and domestic animal species act as natural or accidental hosts. Swine can act as maintenance or accidental hosts of pathogenic Leptospira spp. This study aimed at investigation of Leptospira spp. prevalence and diversity in slaughtered pigs in southern Italy (Sicily). In total, 55 samples of kidneys and blood were collected. Microscopic agglutination test and real-time PCR were performed to detect pathogenic and intermediately pathogenic Leptospira. Partial rpoB gene sequencing and multi-locus sequence typing (MLST) were performed to characterize Leptospira species. The analysis showed a seropositivity rate of 16.4%, with Australis representing the most frequently identified serogroup (63.64%); Pomona and Sejroe were detected with a prevalence of 27.27% and 9.09%, respectively. Pathogenic Leptospiral DNA was detected in 2 kidney samples (3.64%). Leptospira were identified through MLST as L. borgpetersenii serovar Tarassovi (serogroup Tarassovi). Obtained data confirmed the presence of Leptospira infection among pigs in southern Italy, suggesting that management of these animals may be considered an occupational risk for humans.

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Leptospira interrogans serogroup Sejroe serovar Hardjo in aborting cows: two herd cases in Sicily (Italy)

Cited by 13 publications

References 17 publications

Influence of Season, Population and Individual Characteristics on the Prevalence of Leptospira spp. in Bank Voles in North-West Germany

Influence of Season, Population and Individual Characteristics on the Prevalence of Leptospira spp. in Bank Voles in North-West Germany

Leptospira interrogans Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System

Leptospira in Slaughtered Fattening Pigs in Southern Italy: Serological Survey and Molecular Typing

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