“…Although less is known about the intracellular metabolism in the late phase of growth, it is likely that L. pneumophila gets access to carbohydrate sources such as glycogen, glucose and other polymers from the host upon LCV lysis (Lang and Flieger, 2011 ). In addition, the transmissive form of L. pneumophila contains high amounts of PHB, which serves as energy and carbon storage for the maintenance of the intracellular growth cycle (Gillmaier et al, 2016 ; Häuslein et al, 2017 ). Moreover, intracellular L. pneumophila metabolizes myo -inositol, which was reported to promote infection of A. castellanii and macrophages (Manske et al, 2016 ), and engages the translocated protein MavN, which once integrated in the host-derived LCV membrane, facilitates the acquisition of iron into its vacuole (Isaac et al, 2015 ).…”