LeAbsi1 gene expression regulation by various abiotic and oxidative stresses

Abstract

A full-length LeAbsi1 cDNA was isolated from the tomato (Lycopersicon esculentum L.) plant. Phylogenetic analysis was performed, based on the deduced amino acid sequence of the LeAbsi1 cDNA and revealed high amino acid identity (AAID) to unknown proteins in Solanum melongena (87% AAID) and Petunia integrifolia (80% AAID). LeAbsi1 shares high sequence identity with a zinc finger (C2H2-type) family protein from Arabidopsis (53% AAID) and the CaAbsi1 protein in Capsicum annuum (49% AAID). Expression of the LeAbsi1 gene under abiotic and oxidative stresses was investigated in this study, including exposure to the conditions of 200 mM NaCl, 200 mM mannitol, cold temperature (4°C), 100 µM abscisic acid (ABA), 10 mM hydrogen peroxide (H2O2), and 50 µMM methyl vilogen (MV). LeAbsi1 was significantly induced after a 3 h exposure to NaCl, mannitol and low temperature treatment. Its transcripts also accumulated strongly after plant hormone ABA treatment for 1 h. Moreover, LeAbsi1 was significantly induced by MV treatments after 6 h. These observations suggest that the LeAbsi1 gene plays a role in both abiotic and oxidative stress responses.Key words: Abiotic stress, LeAbsi1, tomato, oxidative stress