Lactococcus lactisKR-050L extract suppresses house dust mite induced-atopic skin inflammation through inhibition of keratinocyte and mast cell activation

Abstract

Aims: Atopic dermatitis (AD) is a chronic inflammatory skin disease, with a steadily increasing prevalence. Lactic acid bacteria (LAB) have been widely used in the food industry and are an attractive option for preventing and treating allergic skin diseases. We previously isolated new LABs including Lactococcus lactis KR-050L from Gajuknamu kimchi, and showed the anti-inflammatory effects of extract of L. lactis KR-050L culture broth (LLK). In this study, we investigated the effects of LLK on AD. Methods and Results: For the in vitro study, we used human keratinocytes (HaCaT) and mast cells (RBL-2H3). In vivo study, we investigated the effects of LLK on Dermatophagoides farinae extract (DFE) and 2,4-dinitrochlorobenzene (DNCB)-induced atopic skin inflammation in mice. LLK suppressed expression of pro-inflammatory cytokines and chemokines by down-regulation of p38 MAPK, STAT1 and nuclear translocation of NF-jB in keratinocytes. Topical application of LLK suppressed AD symptoms based on reduction in ear thickness, serum IgE levels and immune cell infiltration. Furthermore, LLK inhibited serum histamine levels and mast cells infiltration in vivo, and reduced mast cells activation in vitro. Conclusions: These results suggest that LLK inhibits AD symptoms through inhibition of keratinocytes and mast cells activation. Significance and Impact of the Study: LLK is a potential therapeutic candidate for AD treatment.

Treatment of atopic dermatitis with probiotic L. lactis lysate - A double-blinded, placebo-controlled pilot study

“…In vitro studies have also shown that Lactococcus lactis KR-050L suppresses pro-inflammatory cytokines and chemokines in keratinocytes by suppressing gene expression responsible for pro-inflammatory cytokine and chemokine production. 29 To our knowledge, our study is the first in vivo study in humans investigatingLactococci in the treatment of AD.…”

Matrine Mediates Inflammatory Response via Gut Microbiota in TNBS-Induced Murine Colitis

“…NF-κB plays critical roles in development, survival, oxidative stress, inflammation and activation of B lymphocytes (Herder et al, 2015; D’Addio and Fiorina, 2016; Sasaki and Iwai, 2016; Jin et al, 2018). NF-κB was identified as one of the key regulators in the immunological setting.…”

Pediococcus pentosaceus KF159 alleviates house dust mite-induced atopic dermatitis by promoting IL10 production and regulatory T cell induction

“…15,16 In particular, it has excellent control effects against allergic diseases including allergic rhinitis, allergic asthma, food allergy, and AD. [17][18][19] Probiotics control immune responses via various mechanisms. They can regulate maturation through contact with DCs and influence their interaction with other immune cells to polarize antigenspecific T cell responses to Th1, Th2, Th17, or regulatory T (Treg) cells.…”