<i>Lactococcus lactis</i>as an adjuvant and delivery vehicle of antigens against pneumococcal respiratory infections

Medina, Marcela; Vintiñi, Elisa; Villena, Julio; Raya, Raúl R.; Álvarez, Susana

doi:10.4161/bbug.1.5.12086

Cited by 42 publications

(25 citation statements)

References 86 publications

(113 reference statements)

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“…Many LAB species are also used as probiotics for human and animal consumption (Gilliland, 1989) and have generally regarded as safe (GRAS) Food and Drug Administration (FDA) status. In addition, most LAB are quite acid resistant and certain strains are able to effectively survive passage through the gastrointestinal (GI) tract, making LAB attractive potential delivery vehicles for recombinant protein vaccines (Medina et al, 2010). However, not all LAB species have a positive impact on food production or human health.…”

Section: Introductionmentioning

confidence: 99%

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

Zeyniyev

Kuipers

et al. 2012

FEMS Microbiol Rev

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Lactic acid bacteria (LAB) are indigenous to food-related habitats as well as associated with the mucosal surfaces of animals. The LAB family Streptococcaceae consists of the genera Lactococcus and Streptococcus. Members of the family include the industrially important species Lactococcus lactis, which has a long history safe use in the fermentative food industry, and the disease-causing streptococci Streptococcus pneumoniae and Streptococcus pyogenes. The central metabolic pathways of the Streptococcaceae family have been extensively studied because of their relevance in the industrial use of some species, as well as their influence on virulence of others. Recent developments in high-throughput proteomic and DNA-microarray techniques, in in vivo NMR studies, and importantly in whole-genome sequencing have resulted in new insights into the metabolism of the Streptococcaceae family. The development of cost-effective high-throughput sequencing has resulted in the publication of numerous whole-genome sequences of lactococcal and streptococcal species. Comparative genomic analysis of these closely related but environmentally diverse species provides insight into the evolution of this family of LAB and shows that the relatively small genomes of members of the Streptococcaceae family have been largely shaped by the nutritionally rich environments they inhabit.

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Section: Introductionmentioning

confidence: 99%

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

Zeyniyev

Kuipers

et al. 2012

FEMS Microbiol Rev

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“…However, mucosal vaccination has several benefits over the parenteral way: it is easy to administer, it can be administered through the same route of entry as pathogens, it makes self-administration possible and is able to induce the systemic and mucosal immune response. The nasal route is undoubtedly the better way to provide immunization against pathogens that enter the body through the respiratory tract and generally an appropriate immune response in the mucosal and systemic compartments is induced against specific antigens [5,20,21]. However, in contrast to parenteral immunization, mucosal immunizations require potent adjuvants to enhance immunogenicity.…”

Section: Discussionmentioning

confidence: 99%

Specific Immune Response Induced by a Lactobacillus Associated with a Pneumococcal Antigen in an “in vitro” Human Cells Model

Vintiñi¹

2012

J Vaccines Vaccin

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In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM) Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.

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“…Lactic acid bacteria have been used as a live oral vaccine expression system for their safety, weak immunogenicity and adjuvant properties (Bermú dez-Humarán, 2009;Hanniffy et al, 2007;Liu et al, 2009;Medina et al, 2010;Wei et al, 2010). As a specific lactic acid bacterium in the human intestinal tract, BI is an advantageous vehicle for mucosal vaccination purposes because it is considered safer than other live vaccine carriers, which have pathogenic variants (e.g.…”

Section: Discussionmentioning

confidence: 99%

Construction of Bifidobacterium infantis as a live oral vaccine that expresses antigens of the major fimbrial subunit (CfaB) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic Escherichia coli

Luo

Xue-ping

et al. 2012

Microbiology

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We sought to develop Bifidobacterium infantis (BI) as a vehicle for the expression of heterologous antigens. Two proteins of enterotoxigenic Escherichia coli (ETEC) were expressed in BI: CfaB, a major fimbrial subunit protein, and LTB, the B subunit of heat-labile enterotoxin. The expression of CfaB and LTB in BI was verified by electrophoretic analysis. Sprague-Dawley rats were then subjected to intragastric immunization with BI-CfaB and BI-LTB systems both separately and together. ELISA was used to characterize the serum and mucosal immune responses against ETEC antigens. The immunized rats were intraperitoneally challenged with wild-type ETEC H10407 to study the immune response in vivo. The serum titres of IgG and faecal IgA antibodies in the BI-CfaB plus BI-LTB mixed vaccination group were significantly greater than those in the other two groups, which were immunized with a single vaccine (P,0.05). However, no significant difference was seen between the two groups that received a single immunization. These results suggest that expressing CfaB and LTB in BI provides a probiotic system with immunogenic properties. Furthermore, the expression of LTB in BI preserved its mucosal adjuvant effect. So this study confirms that BI can be used as a novel oral vaccine expression system for a heterologous antigen and BI-LTB can provide mucosal adjuvant properties.

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Lactococcus lactisas an adjuvant and delivery vehicle of antigens against pneumococcal respiratory infections

Cited by 42 publications

References 86 publications

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

From meadows to milk to mucosa – adaptation ofStreptococcusandLactococcusspecies to their nutritional environments

Specific Immune Response Induced by a Lactobacillus Associated with a Pneumococcal Antigen in an “in vitro” Human Cells Model

Construction of Bifidobacterium infantis as a live oral vaccine that expresses antigens of the major fimbrial subunit (CfaB) and the B subunit of heat-labile enterotoxin (LTB) from enterotoxigenic Escherichia coli

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