Lactobacillus rhamnosus2016SWU.05.0601 regulates immune balance in ovalbumin‐sensitized mice by modulating expression of the immune‐related transcription factors and gut microbiota

Abstract

BACKGROUND Probiotics regulate host immune balance, which may reduce immune‐related diseases. The effects and mechanisms of Lactobacillus rhamnosus 2016SWU.05.0601 (Lr‐0601) on the immune response in ovalbumin (OVA)‐sensitized mice were explored. RESULTS Lr‐0601 reduced serum immunoglobulin (Ig)E and OVA‐IgE and attenuated the alteration in lung pathology in OVA‐sensitized mice. Lr‐0601 blocked OVA‐induced up‐regulation in serum T helper (Th) 2 and Th17 cytokines but increased the serum levels of Th1 and regulatory T (Treg) cytokines in OVA‐sensitized mice. OVA also markedly reduced the protein levels of spleen T‐box transcription factor and forkhead/winged helix transcription factor p3, leading to the reduced mRNA expression of interferon‐γ and interleukin (IL)‐10. By contrast, OVA markedly increased the protein expression of spleen GATA‐binding protein 3 and retinoid‐related orphan receptor γt, as well as the mRNA expression of spleen IL‐4 and IL‐17. These changes induced by OVA were reversed by Lr‐0601. Moreover, Lr‐0601 helped alleviate OVA‐induced intestinal microbiota dysbiosis. A correlation was found between specific genera and immune‐associated cytokines. CONCLUSION The combined results indicate that Lr‐0601 modulated the balance of Th1/Th2 and Treg/Th17 in OVA‐sensitized mice, which was associated with the regulation of immune‐related transcription factors and gut microbiota. Lr‐0601 can potentially be used as a probiotic for preventing immune‐related diseases. © 2020 Society of Chemical Industry

Probiotic strains alleviated OVA-induced food allergy in mice by regulating the gut microbiota and improving the level of indoleacrylic acid in fecal samples

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“…According to a meta-analysis encompassing 17 studies, probiotics may significantly lower the risk of infantile eczema, thus suggesting a new potential indication for probiotic use in pregnancy and infancy ( Zuccotti et al, 2015 ). Recently, L. rhamnosus Lr-0601 proved to be able to modulate the balance of Th1/Th2 and Treg/Th17 in ovalbumin-sensitized mice ( Song et al, 2020 ). In mouse model with FA induced by ovalbumin, Li N. et al (2020) observed an abnormal gut bacterial composition, accompanied by increased immunoglobulin G, immunoglobulin E, and interleukin-4/interferon-gamma.…”

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“…Administration of C. butyricum CGMCC0313-1 significantly elevated sIgA and Foxp3 + Treg cells in the spleen in mice with β-lactoglobulin (BLG) sensitivity and markedly reduced anaphylactic symptoms [109]. L. rhamnosus 2016SWU.05.0601 has recently been demonstrated by Song et al to modulate the expression of immune-related transcription factors and gut microbiota, consequently regulating the imbalance of Th1/Th2 and Treg/Th17 in OVA-sensitized mice [110]. Treatment with a probiotics mixture (L. lactis KF140, Pediococcus pentosaceus KF159, L. pentosus KF340, L. paracasei 698, and Bacillus amyloliquefaciens 26N) significantly suppressed the OVA-induced allergic symptoms, inhibited the release of IgE and Th2 cytokines, and promoted the development of Foxp3 + Tregs in mice [111].…”