Lactobacillus rhamnosusReduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats

Abstract

Some lactic acid bacteria (LAB) are observed to be potential probiotics with functional properties such as lowering fasting blood glucose (FBG), as a promising hyperglycemia management. This study investigated the ability and mechanism of Lactobacillus rhamnosus BSL and Lactobacillus rhamnosus R23 on lowering FBG in diabetic rats induced by streptozotocin (STZ). The rats were orally administered with L. rhamnosus BSL and L. rhamnosus R23 by giving 1 mL cell suspension (109 CFU/mL) daily for 30 days. The body weight (BW) was recorded once in three days, and FBG was recorded once in six days. An oral glucose tolerance test (OGTT) was measured 1 week after injection with STZ and before sacrifice. Fecal samples were collected on days 0, 15, and 30 for LAB population and identification, performed by PCR detecting 16S rRNA. Oral administration of L. rhamnosus BSL and L. rhamnosus R23 decreased FBG and improved glucose tolerance via downregulation of glucose-6-phosphatase (G6pc) expression by 0.57- and 0.60-fold change, respectively (P<0.05). The lipid profiles, BUN, creatinine, SGOT, and SGPT were significantly (P<0.05) different between normal and diabetic rats, but they were not significantly (P>0.05) different among diabetic rats. Both strains were effective in increasing fecal LAB population. Molecular identification of the isolated LAB from fecal sample indicated that they were able to survive and pass through the digestive tract. These results suggested that both strains have the ability to manage blood glucose level and become a promising agent to manage hyperglycemia and diabetes.

Transplantation of an obesity-associated human gut microbiota to mice induces vascular dysfunction and glucose intolerance

“…Lactobacillus has been used to improve glucose tolerance independent of weight loss in db/db mice, 34 and, more recently, oral supplementation with Lactobacillus rhamnosus has reduced gluconeogenesis and improved hyperglycemia in diabetic rats. 35 The reduction in glucose tolerance in recipient mice is interesting given that obese donors did not display elevations in fasting glucose or in HbA1C, a marker of chronic glucose levels. These data align with those by Vrieze et al, who demonstrated that transfer of gut microbiota from lean human donors to individuals with metabolic syndrome increased insulin sensitivity in the latter.…”

Goat Milk Improves Glucose Homeostasis via Enhancement of Hepatic and Skeletal Muscle AMP‐Activated Protein Kinase Activation and Modulation of Gut Microbiota in Streptozocin‐Induced Diabetic Rats

“…[22,23] Additionally, supplementation of Lactobacillus may benefit T1D www.advancedsciencenews.com www.mnf-journal.com patients. [24] There are several proposed mechanisms through which Lactobacillus improves glucose metabolism, including decreasing inflammatory status, improving insulin signaling, and SCFAs production. [25][26][27] In this study, we did find that goat milk consumption (at 5 and 10 g kg −1 doses) decreased the levels of TNF-, which is an indicator of inflammatory status (Table 2).…”

Maintaining Digestive Health in Diabetes: The Role of the Gut Microbiome and the Challenge of Functional Foods

“…L. rhamnosus BSL and L. rhamnosus R23, when administered in STZ-induced diabetic Sprague-Dawley rats, led to elevated LAB levels after 30 days of probiotic supplementation and improved glucose tolerance and glucose control, as FBG was significantly reduced. After probiotic treatment, there was a decrease in TC and in atherogenic index [ 130 ].…”