<i>Lactobacillus reuteri</i>DSM 17938 in Infantile Colic: A Randomized, Double-Blind, Placebo-Controlled Trial

Savino, Francesco; Cordisco, Lisa; Tarasco, Valentina; Palumeri, E; Calabrese, R.; Oggero, R; Roos, Stefan; Matteuzzi, Diego

doi:10.1542/peds.2010-0433

Cited by 305 publications

(336 citation statements)

References 27 publications

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“…To fill this gap, this study investigated the composition of microbiota, with respect to specific groups of colonic bacteria claimed to exert an influence on infantile colic of breastfed infants (16), in formula-fed infants with and without colic. For this reason, we chose to use fluorescent in situ hybridisation (FISH) to enumerate coliforms (Enterobacteriaceae), lactic acid bacteria (LAB) and bifidobacteria, in order to establish whether the extent of colonisation differed between the two groups.…”

Section: Key Notesmentioning

confidence: 99%

Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Savino¹,

Quartieri

Marco³

et al. 2016

Acta Paediatrica

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Aim: This study compared the faecal microbial composition of formula-fed infants who did and did not have colic.Methods: Faecal samples from formula-fed infants under 16 weeks of age with (n = 38) and without (n = 39) colic were collected at Department of Pediatrics in Turin, Italy, between February 2014 and October 2015. The pH and faecal ammonia were determined and total bacteria, bifidobacteria, lactic acid bacteria and coliforms were quantified by fluorescent in situ hybridisation (FISH).Results: Faecal ammonia was significantly higher in the colicky infants than in the controls (483 vs. 216 lg/g, p < 0.05). The FISH counts of total bacteria were lower in colicky infants (1.8E10 AE 1.5E10) than in the controls (3.4E10 AE 3.0E10) (p < 0.05). The relative abundance of coliform bacteria was significantly higher in colicky infants (p < 0.05). No differences were observed for the bifidobacteria and lactic acid bacteria counts between the two groups.Conclusion: Our comparison of formula-fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia. This study provides the stimulus for further studies of the gut microbiome, using new methods of analysis such as 16S metagenomics sequencing in order to lead to more tailored dietary approaches.

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Section: Key Notesmentioning

confidence: 99%

Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Savino¹,

Quartieri

Marco³

et al. 2016

Acta Paediatrica

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“…Although data on gut health in infants are scanty [12,13,15,16], many recent clinical trials suggest that probiotic administration in early infancy could be useful for the prevention and treatment of many disorders: modulation of the intestinal microbiota through use of probiotics has been suggested as strategy for preventing NEC, atopic eczema and managing infantile colic symptoms.…”

Section: Discussionmentioning

confidence: 99%

Probiotics and gut health in infants: A preliminary case–control observational study about early treatment with Lactobacillus reuteri DSM 17938

Savino

Fornasero

Ceratto

et al. 2015

Clinica Chimica Acta

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AIMS: we performed this case-control observational study to evaluate the effects of early administration of Lactobacillus reuteri DSM 17938 on microbial composition in infants' gastrointestinal tract. METHODS: early fecal microbiota composition was analyzed by using selective and differential cultural methods. Genomic DNA from positive Escherichia coli and Cronobacter sakazakii colonies was extracted and DNA was processed by multiplex PCR assay. RESULTS: fecal samples of 30 hospitalized infants who previously received probiotics and 30 not receiving probiotics were analyzed. We find that the two groups showed differences in gut microbial strains composition and richness. Infant treated with probiotics have a lower total anaerobic gramnegative counts (p=0.03) and a higher total anaerobic gram-positive counts (p=0.02). Enterobacteriaceae and enterococci were significantly higher (p=0.04) in the control group. No significant differences were observed for total aerobic counts, lactobacilli and bifidobacteria. C. sakazaki was found only in one infant recruited in the control group. Infants not previously treated with probiotics showed a higher colonization by diarrheagenic E. coli (EPEC) (p=0.04). CONCLUSIONS: our findings enhanced our understanding of the effects of probiotics on gut health in pediatric subjects. Early administration of L.reuteri in infancy could improve gut health by reducing pathogens colonization. Our trial underlines the efficacy of an early probiotic administration in improving gut health in infancy.Informed consent was obtained from each child's parent.The study has been approved by a research ethics committee.There are no prior publications or submissions with any overlapping information, including studies and patients.These data have not been published previously and are not under consideration for publication by any other journal. The manuscript has not been and will not be submitted to any other journal while it is under consideration by Clinica Chimica Acta Journal.All authors have read and approved the version of the article being submitted.No an honorarium, grant, or other form of payment was given to anyone to produce the manuscript.All authors declare no conflict of interest.No affiliations with or financial involvement in any organization or entity with a direct financial interest in the subject matter or materials of the research discussed in the manuscript were present. Probiotics and gut health in infants: a case-control observational study about early treatment with Lactobacillus reuteri DSM 17938.

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“…• Third, there is growing evidence that the intestinal microbiota in colicky infants differ from those in healthy controls, since higher levels of anaerobic bacteria, such as coliform and Escherichia coli, and a lower concentration of Lactobacilli have been reported in infants with colic (Savino 2010a;Savino 2013b).…”

Section: Description Of the Conditionmentioning

confidence: 99%

“…Given the growing evidence that the intestinal microbiota in colicky infants differ from those in healthy controls, it is proposed that supplying probiotic bacteria can redress this balance and provide a healthier intestinal microbiota landscape (Savino 2010a;Savino 2013a, Savino 2013b. As the evidence base suggests, common factors impact this colonisation process, such as birth by caesarean section (Grönlund 1999), and it is proposed that offering probiotics prophylactically to all as a form of primary prevention could offer significant benefit to the population with minimal risk.…”

Section: How the Intervention Might Workmentioning

confidence: 99%

Probiotics to prevent infantile colic

Banks

Thomas

Gordon

et al. 2016

Cochrane Database of Systematic Reviews

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Lactobacillus reuteriDSM 17938 in Infantile Colic: A Randomized, Double-Blind, Placebo-Controlled Trial

Abstract: L. reuteri DSM 17 938 at a dose of 10(8) colony-forming units per day in early breastfed infants improved symptoms of infantile colic and was well tolerated and safe. Gut microbiota changes induced by the probiotic could be involved in the observed clinical improvement.

Cited by 305 publications

References 27 publications

Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Probiotics and gut health in infants: A preliminary case–control observational study about early treatment with Lactobacillus reuteri DSM 17938

Probiotics to prevent infantile colic

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