Lactobacillus plantarumLG42 Isolated from Gajami Sik-Hae Inhibits Adipogenesis in 3T3-L1 Adipocyte

Abstract

We investigated whether lactic acid bacteria isolated from gajami sik-hae (GLAB) are capable of reducing the intracellular lipid accumulation by downregulating the expression of adipogenesis-related genes in differentiated 3T3-L1 cells. The GLAB, Lactobacillus plantarum LG42, significantly decreased the intracellular triglyceride storage and the glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner. mRNA expression of transcription factors like peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α involved in adipogenesis was markedly decreased by the GLAB treatment. Moreover, the GLAB also decreased the expression level of adipogenic markers like adipocyte fatty acid binding protein (aP2), leptin, GPDH, and fatty acid translocase (CD36) significantly. These results suggest that the GLAB inhibits lipid accumulation in the differentiated adipocyte through downregulating the expression of adipogenic transcription factors and other specific genes involved in lipid metabolism.

Anti-Obesity Effects of Starter Fermented Kimchi on 3T3-L1 Adipocytes

“…plantarum in FKS have acid and bile tolerance, antioxidative effects, and anti-proliferative effects on HT-29 human colorectal cancer cells (18). Also, anti-obesity effect of the above starters on 3T3-L1 cells and animals were previously reported (4,19,20). Lee et al (21) reported Leu.…”

“…plantarum has been reported to reduce adipogenesis and lipogenesis by inhibiting the activations of PPAR-, C/EBP, and FAS in 3T3-L1 adipocytes (19,20), Leuconostoc sp. and Lactobacillus sp.…”

Pyrosequencing analysis of microbiota reveals that lactic acid bacteria are dominant in Korean flat fish fermented food,gajami-sikhae

“…[43][44][45][46][47] In accordance with this, L. plantarum LG42 isolated from gajami-sikhae showed probiotic effects including anti-obesity properties. 7,11) A strain of L. sakei isolated from another food had been evaluated for its probiotic potential such as gastric resistance, survival, and bacteriocin production in the presence of bile salts and commercial prebiotics, and reducing the adhesion of Listeria monocytogenes to intestinal epithelial cells. 48) Therefore, we need to characterize the L. sakei strain isolated from gajami-sikhae to determine its potential probiotic effects.…”

“…10) Lactobacillus plantarum LG42, a lactic acid bacterium (LAB), was isolated from gajami-sikhae and its antiobesity effect on high-fat-diet-induced obese mice was investigated. 7,11) The high-fat-diet-induced obese mice group supplemented with L. plantarum LG42 showed lower body weight and reduced epididymal and back fat as compared *Corresponding author. Email: lmk123@kfri.re.kr Abbreviations: ACC, acetyl CoA carboxylase; CPT-I, carnitine palmetonyltransferase-I; DGGE, denaturing gradient gel electrophoresis; LAB, lactic acid bacterium; LXRα, liver X receptor α; NGS, next-generation sequencing; OTU, operational taxonomic unit; PCoA, principal coordinate analysis; PPARα, peroxisome proliferator-activated receptor α; SREBP-1, sterol regulatory element-binding protein-1.…”

Pyrosequencing-based analysis of the bacterial community during fermentation of Alaska pollock sikhae: traditional Korean seafood

“…While Kyoungsangdo-style recipes use dried Alaska pollock, undried fish are utilized for the Kangwondo-style recipes. Despite its importance as a Korean fermented food, there is limited research regarding sikhae and our understanding of the fermentation mechanisms of sikhae is largely incomplete.Novel bacterial strains were isolated from sikhae, mainly from gajami-sikhae (Kim et al, 2009;Jung et al, 2012a;Shin et al, 2011;Park et al, 2013Park et al, , 2014. Alishewanella jeotgali MS1 T was isolated from gajami-sikhae and its genome was fully sequenced (Kim et al, 2009;Jung et al, 2012a).…”