Lactobacillus crispatus-derived exopolysaccharides with antibacterial activity limit Salmonella typhimurium invasion by inhibiting inflammasome-mediated pyroptosis

Abstract

A Lactobacillus crispatus-derived exopolysaccharide (EPS 7-4) has considerable potential as a natural antibacterial, anti-inflammatory, and health-promoting functional food or medicine.

Self-Assembling Sulfated Lactobacillus Exopolysaccharide Nanoparticles as Adjuvants for SARS-CoV-2 Subunit Vaccine Elicit Potent Humoral and Cellular Immune Responses

“…The structure and characterization of EPS 8-2 were analyzed in this study, and EPS 7-4 has been analyzed in our previous studies. 33 The UV maximum absorption peaks of EPS 8-2 appeared at 190−210 nm, and no absorption peaks at 260 and 280 nm were present (Figure S2A). In addition, the average M w and number-average molecular weight (M n ) of EPS 8-2 were 45.74 and 44.70 kDa, respectively.…”

“…The structure and characterization of EPS 8-2 were analyzed in this study, and EPS 7-4 has been analyzed in our previous studies . The UV maximum absorption peaks of EPS 8-2 appeared at 190–210 nm, and no absorption peaks at 260 and 280 nm were present (Figure S2A).…”

“…31,32 Our previous study showed that Lactobacillus EPS had effective immunomodulatory effects, suggesting the potential of Lactobacillus EPS as a vaccine adjuvant. 33 The immunomodulatory capacity of EPS is related to the molecular weight, monosaccharide composition, chemical composition, and structure. 34 For example, EPS containing α-(1 → 2) glycosidic bonds promoted phagocytosis by macrophages, whereas EPS containing β-(1 → 3) and β-(1 → 6) glycosidic bonds activated the production of inflammatory factors.…”

“…Lactobacillus EPSs are known to be safe, have good biological activities, and are widely used in food and medical applications. , Our previous study showed that Lactobacillus EPS had effective immunomodulatory effects, suggesting the potential of Lactobacillus EPS as a vaccine adjuvant . The immunomodulatory capacity of EPS is related to the molecular weight, monosaccharide composition, chemical composition, and structure .…”

“…Lactobacillus was isolated from wild Gallus gallus feces in the Jiangsu Yancheng National Rare Birds Nature Reserve and saved in our lab. EPS was purified from the Lactobacillus (Lactobacillus johnsonii 3-1, Lactobacillus crispatus 7-4, Lactobacillus salivarius 8-2, Lactobacillus reuteri 9-2, and Lactobacillus plantarum 20-1) culture as previously described . The precipitated EPS was dissolved in deionized water and dialyzed (MW cutoff 8000–14,000 Da, YA1071, Solarbio) against deionized water at 4 °C for 48 h to remove the salts and dried in a vacuum-freezing dryer to obtain EPS.…”