Lactobacillus bulgaricus improves antioxidant capacity of black garlic in the prevention of gestational diabetes mellitus: a randomized control trial

Abstract

Objectives: Lactobacillus bulgaricus may improve antioxidant capacity of black garlic in the prevention of gestational diabetes mellitus (GDM).Methods: Black garlic was prepared with or without L. bulgaricus. Volatile and polysaccharides were analyzed by using LC-MS, Fourier Transform infrared (FTIR) and 13C nuclear magnetic resonance (NMR). The study design was parallel randomized controlled trial and 226 GDM patients were randomly assigned into BG (black garlic and L. bulgaricus) and CG (black garlic) groups, and allocation ratio was 1:1. The treatment duration was 40 weeks. Fasting blood glucose (FBG) and 1- and 2-h blood glucose (1hBG and 2hBG) after oral glucose tolerance test (OGTT) were detected. Antioxidant function of black garlic was determined by measuring plasma malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC) in GDM patients. The comparison between two groups was made using two independent samples t test.Results: The intake of nutrients was similar between two groups (P>0.05). L. bulgaricus promoted the transformation of the glucopyranoside to glucofuranoside. L. bulgaricus increased the abilities of black garlic for scavenging hydroxyl radicals, 2,2′-azino-bis (3-ethylbenzenthiazoline-6-sulfonic) acid (ABTS) and DPPH free radicals. L. bulgaricus reduced the levels of FBG, 1hBG and 2hBG, and incidence of perinatal complications (P<0.01). Plasma MDA level in the BG group was lower than in the CG group, whereas the levels of SOD, GSH-PX and T-AOC in the BG group were higher than in the CG group (P<0.01).Conclusions: L. bulgaricus improves antioxidant capacity of black garlic in the prevention of GDM.

Effects of phycocyanin on pulmonary and gut microbiota in a radiation-induced pulmonary fibrosis model

“…For example, Lactobacillus interacts with many pathogens by secreting salivary hormone B (an antibiotic) [ 44 ]. Other studies have found that the fermentation of lactic acid bacteria can not only enhance the physiological activity of intestinal peristalsis, but also improve immunity and avoid hyperglycemia [ 45 , 46 ]. Bifidobacterium is also a beneficial bacteria in the intestinal tract, and it has immuno-modulation and hypoglycemic effects [ 47 ].…”

Determination of Polyphenols in Ilex kudingcha and Insect Tea (Leaves Altered by Animals) by Ultra-high-performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UHPLC-QqQ-MS) and Comparison of Their Anti-Aging Effects

“…The lipid peroxidation metabolite MDA can damage biological macromolecules such as proteins and nucleic acids, causing the body to age and develop various diseases. The level of MDA indirectly reflects the degree of free radical attack on the body cells, and it is another important indicator of the degree of oxidative damage in the body ( Si et al, 2019 ). Reducing the MDA content in tissues and serum are of great significance to alleviate oxidative damage.…”

Black Garlic and Its Bioactive Compounds on Human Health Diseases: A Review

“…Moreover, Lee et al [99] observed that aged garlic extract significantly decreased thiobarbituric acid reactive substances levels and elevated the activities of SOD, glutathione peroxidase (GSH-Px), and catalase (CAT) in diabetic mice. The results obtained by Si et al [100] demonstrated that 40 weeks of supplementation of BG lowered the levels of plasma malondialdehyde, SOD, GSH-Px, and total antioxidant capacity in pregnant women. They also mentioned that Lactobacillus bulgaricus improved the antioxidant capacity of BG in the prevention of gestational diabetes mellitus.…”