Lactiplantibacillus plantarum X7022 ameliorates loperamide‐induced constipation and modulates gut microbiota in mice

Abstract

Lactiplantibacillus plantarum X7022 is a novel strain isolated from stinky tofu brine. Its constipation amelioration potential was evaluated on two different constipation types (acute and subacute) in mice induced by loperamide hydrochloride in present work. L. plantarum X7022 could significantly decrease the first black stool defecation time, and increase black fecal weight, number, and the gastrointestinal transit ratio in both models. High‐dose treatment of L. plantarum X7022 (2 × 1010 CFU/kg BW) was more significant in improving constipation condition than low‐dose (2 × 107 CFU/kg BW). Furthermore, 16S rDNA sequencing of the intestinal flora showed that the treatment with the strain X7022 could increase the α diversity in both constipation models. Meanwhile, the abundance of Bacteroidetes increased, and Firmicutes decreased, which was more effective in acute constipated mice. In addition, X7022 treatment could enhance the relative abundance of beneficial flora, such as Bacteroides, Ruminococcus, and depressed Adlercreutzia and Odoribacter in acute model mice. In subacute constipated mice, X7022 treatment could enhance Alloprevotell, Rikenellaceae_RC9, and decreased Lachnospiraceae_NK4A136 and Desulfovibrio. Therefore, L. plantarum X7022 is a prospect probiotic strain for the amelioration of constipation.

Editorial: Probiotics and constipation

“…Dysbiosis of intestinal microbiota has been associated with constipation, and the restoration of gut microbiota homeostasis is proposed as a promising strategy for the treatment of constipation (4,5). Currently, animal and clinical studies have shown that several probiotics (live microorganisms) can attenuate constipation by regulating the gut microbiota (6)(7)(8). However, the identification, effectiveness evaluation, and underlying mechanism of novel probiotics relieving constipation are still necessary because of the strain specificity of probiotic effects.…”