<i>Kocuria</i> Species Peritonitis: Although Rare, we Have to Care

Dotis, John; Printza, Nikoleta; Stabouli, Stella; Papachristou, Fotiοs

doi:10.3747/pdi.2013.00138

Cited by 25 publications

(13 citation statements)

References 16 publications

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“…and Rothia spp. grow aerobically in both the environment and the oral cavity [ 40 , 41 ] while Streptococci or Actinomyces spp. usually prefer an anaerobic environment, which is not provided by a toothbrush.…”

Section: Discussionmentioning

confidence: 99%

The Toothbrush Microbiome: Impact of User Age, Period of Use and Bristle Material on the Microbial Communities of Toothbrushes

2020

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Toothbrushes play a central role in oral hygiene and must be considered one of the most common articles of daily use. We analysed the bacterial colonization of used toothbrushes by next generation sequencing (NGS) and by cultivation on different media. Furthermore, we determined the occurrence of antibiotic resistance genes (ARGs) and the impact of different bristle materials on microbial growth and survival. NGS data revealed that Enterobacteriaceae, Micrococcaceae, Actinomycetaceae, and Streptococcaceae comprise major parts of the toothbrush microbiome. The composition of the microbiome differed depending on the period of use or user age. While higher fractions of Actinomycetales, Lactobacillales, and Enterobacterales were found after shorter periods, Micrococcales dominated on both toothbrushes used for more than four weeks and on toothbrushes of older users, while in-vitro tests revealed increasing counts of Micrococcus on all bristle materials as well. Compared to other environments, we found a rather low frequency of ARGs. We determined bacterial counts between 1.42 × 106 and 1.19 × 107 cfu/toothbrush on used toothbrushes and no significant effect of different bristles materials on bacterial survival or growth. Our study illustrates that toothbrushes harbor various microorganisms and that both period of use and user age might affect the microbial composition.

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Section: Discussionmentioning

confidence: 99%

The Toothbrush Microbiome: Impact of User Age, Period of Use and Bristle Material on the Microbial Communities of Toothbrushes

2020

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“…Twenty-two unique genera were detected in the rinse samples of pSS patients; most of the species, such as Kocuria rosea , Cupriavidus gilardii and Anaerococcus prevotii , are opportunistic pathogens that can cause infectious processes ( Savini et al, 2010 ; Chiu & Wang, 2013 ; Tennert et al, 2014 ; Dotis et al, 2015 ; Du et al, 2015 ; Zhang et al, 2017 ), particularly in immunocompromised hosts. Though the pathogenicity of such species is known to us to some extent, the relevance to pSS and dental caries remains undetermined.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of oral microflora in patients with primary Sjögren’s syndrome by using high-throughput sequencing

Zhou

Ling

Ding

et al. 2018

PeerJ

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BackgroundThe objective of this study was to characterize the oral microflora profile of primary Sjögren’s syndrome (pSS) patients, thereby revealing the connection between oral bacterial composition and dental caries, and to identify the “core microbiome” in the oral cavities of pSS patients and systemic healthy individuals by using a high-throughput sequencing technique.MethodsTwenty-two pSS patients and 23 healthy controls were enrolled in this study. Their clinical data and oral rinse samples were collected. The V3–V4 hypervariable regions of the bacterial 16S rRNA gene of samples were amplified and analyzed by high-throughput sequencing on the Illumina Miseq PE300 platform.ResultsBoth two groups were age- and sex-matched. There were significantly higher decayed, missing and filled teeth (DMFT) and decayed, missing and filled surfaces (DMFS) in the pSS group than in the control group (p < 0.01). Alpha diversity was depleted in pSS patients, compared with healthy controls (p < 0.01), while beta diversity between the two groups was not significantly different. Seven discriminative genera (LDA > 4) were found between the two groups in LEfSe (LDA Effect Size) analysis. The relative abundance of Veillonella in pSS patients was fourfold higher, while Actinomyces, Haemophilus, Neisseria, Rothia, Porphyromonas and Peptostreptococcus were significantly lower in pSS patients than in healthy controls. However, the correlation between Veillonella and DMFT/DMFS was not significant (p > 0.05). In Venn diagram analysis, nine genera shared by all samples of two groups, which comprised 71.88% and 67.64% in pSS patients and controls, respectively.DiscussionThese findings indicate a microbial dysbiosis in pSS patients; notably, Veillonella might be recognized as a biomarker in pSS patients. The core microbiome in pSS patients was similar to the systemic healthy population. These provide insight regarding advanced microbial prevention and treatment of severe dental caries in pSS patients. This study also provides basic data regarding microbiology in pSS.

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“…In the present study, antibiotics have not undergone susceptibility tests against Micrococci, Rothiae, Kocuria kristinae, and Burkholderia cepacia. This is because automated identification systems -although they allow accurate identificationunfortunately, do not perform susceptibility tests for some bacterial species, the fact that remains one of the most important deficiencies of these systems [19,20]. There are not adequate recent studies to indicate the pattern of Micrococcal response to antibiotics, but it is convenient here to refer to the Dürst etal findings which showed the response of Micrococcus luteus to penicillin [21].…”

Section: Discussionmentioning

confidence: 99%

Incidence and susceptibility patterns of urine bacterial flora in young females

Shami¹,

Al-Mijalli²,

Somily

et al. 2020

Preprint

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Background: It has been established that the urinary tract is not sterile, however, research related to the study of urine bacteria are limited. Our work aims to study the frequency and patterns of resistance of normal urinary aerobic bacterial flora.Methods: Clean catch midstream urine specimens were collected from 120 young healthy females, and then cultured. Identification of bacteria and antimicrobial susceptibility were done by means of Biomérieux VITEK® 2 automated systems.Subjects who have undergone antimicrobial treatment in one month weren't included.Results: The incidence of positive bacterial cultures was 54.2%, of which 21.5% were ploymicrobial. 107 bacterial isolates that encompass 12 genera and 27 species that were predominated by Gram-positive bacteria (72%) were cultivated. Staphylococcaceae (46.1%) and Enterobacteriaceae (17.8%) were the most frequently isolates among Gram positive and Gram negative bacteria respectively, from them 36 species have been identified as b lactamase producers. The top four frequently isolated bacteria are Micrococcus species (16%), Staphylococcus haemolyticus (13.2%), Staphylococcus aureus (10%), and Klebsiella pneumoniae (10%). Twenty two bacterial species weresubjected to antimicrobial susceptibility testing by using broad and narrow spectrum antibiotics and antimicrobials. Ampicillin showed the lowest susceptibility rate against Gram positives, followed by erythromycin and azithromycin. The lesser antimicrobial susceptibility potential among Gram negative bacteria was exhibited against ampicillin, followed by piperacillin and cefotaxime. Conclusion:Our findings emphasize the importance of highlighting urine bacterial flora in research especially those related to susceptibility patterns, employing more advanced culture methods as multiple drug resistant bacteria were isolated.

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Kocuria Species Peritonitis: Although Rare, we Have to Care

Cited by 25 publications

References 16 publications

The Toothbrush Microbiome: Impact of User Age, Period of Use and Bristle Material on the Microbial Communities of Toothbrushes

The Toothbrush Microbiome: Impact of User Age, Period of Use and Bristle Material on the Microbial Communities of Toothbrushes

Molecular analysis of oral microflora in patients with primary Sjögren’s syndrome by using high-throughput sequencing

Incidence and susceptibility patterns of urine bacterial flora in young females

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