<i>Klebsiella pneumoniae</i>: Prevalence, Reservoirs, Antimicrobial Resistance, Pathogenicity, and Infection: A Hitherto Unrecognized Zoonotic Bacterium

Hu, Yujie; Anes, João; Devineau, Stéphanie; Fanning, Séamus

doi:10.1089/fpd.2020.2847

Cited by 46 publications

(28 citation statements)

References 270 publications

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“…K. pneumoniae were also found to be resistant to colistin, a last-line antibiotic ( Antoniadou et al, 2007 ; Neuner et al, 2011 ). The emergence of AMR in K. pneumoniae against critically important classes of antibiotics represents a major threat to conventional clinical therapy ( Li et al, 2014 ; Hu et al, 2020 ). Novel antibacterial strategies are required to overcome this challenge ( Adamo and Margarit, 2018 ; Mulani et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae

et al. 2021

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Infections associated with antimicrobial-resistant bacteria now represent a significant threat to human health using conventional therapy, necessitating the development of alternate and more effective antibacterial compounds. Silver nanoparticles (Ag NPs) have been proposed as potential antimicrobial agents to combat infections. A complete understanding of their antimicrobial activity is required before these molecules can be used in therapy. Lysozyme coated Ag NPs were synthesized and characterized by TEM-EDS, XRD, UV-vis, FTIR spectroscopy, zeta potential, and oxidative potential assay. Biochemical assays and deep level transcriptional analysis using RNA sequencing were used to decipher how Ag NPs exert their antibacterial action against multi-drug resistant Klebsiella pneumoniae MGH78578. RNAseq data revealed that Ag NPs induced a triclosan-like bactericidal mechanism responsible for the inhibition of the type II fatty acid biosynthesis. Additionally, released Ag+ generated oxidative stress both extra- and intracellularly in K. pneumoniae. The data showed that triclosan-like activity and oxidative stress cumulatively underpinned the antibacterial activity of Ag NPs. This result was confirmed by the analysis of the bactericidal effect of Ag NPs against the isogenic K. pneumoniae MGH78578 ΔsoxS mutant, which exhibits a compromised oxidative stress response compared to the wild type. Silver nanoparticles induce a triclosan-like antibacterial action mechanism in multi-drug resistant K. pneumoniae. This study extends our understanding of anti-Klebsiella mechanisms associated with exposure to Ag NPs. This allowed us to model how bacteria might develop resistance against silver nanoparticles, should the latter be used in therapy.

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Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae

et al. 2021

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“…Klebsiella pneumoniae is one of the most common opportunistic pathogens and can cause a variety of human infections, including lung, urinary tract, bloodstream, and surgical site infections [29,30]. By the emergence of carbapenems resistance, the management of infections to Klebsiella pneumoniae has been intractable and complicated [31].…”

Section: Discussionmentioning

confidence: 99%

Disinfection Strategies of Carbapenem-resistant Klebsiella Pneumoniae in a Healthcare Facility

Zhang

Shen

et al. 2022

Preprint

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Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) infections have become a major global public health challenge. Disinfectants play an important role in controlling horizontal transmission in healthcare facilities. However, the long and extensive routine use of disinfectants, and far less regulated, leading to concern about the development of disinfectant resistance. We detected the susceptibility of CRKP strains against seven kinds of disinfectants commonly used in clinic to guide and assist the infection control work in hospital. Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests of seven kinds of disinfectants (0.1% benzalkonium bromide, 4% aqueous chlorhexidine, 75% alcohol, entoiodine II, 2% glutaraldehyde, 2000mg/L chlorine-containing disinfectants and 3% hydrogen peroxide) were detected by broth dilution method. Three efflux pump genes (oqxA、oqxB、and qacE△1-sul1) were detected by PCR. Results: A total of 162 strains of non-repetitive CRKP strains were isolated in SYS Memorial Hospital from 2015 to 2019. The MBCs expressed by diluted multiples of 64 CRKP strains against the seven kinds of disinfectants were 1-128, 16-32768, 2-64, 16-4096, 4-64, 2-8 and 64-2048, respectively. Aqueous chlorhexidine should be ≥ 2.5 g/L when rinsing or gargling mucous membrane and wound, 0.1% benzalkonium bromide should avoided be diluted, and chlorine-containing disinfectants should be ≥ 1000 mg/L. The mean MIC value of aqueous chlorhexidine from intensive care unit (ICU) (0.0034%) was significantly higher than that from non-ICU (0.0019%) (p < 0.05). The positive rates of three efflux pump genes oqxA, oqxB and qacE△1-sul1 were 68.8% (44/64), 15.6% (10/64) and 89.1% (57/64), respectively.Conclusion: The CRKP strains isolated showed extensive resistance to clinically used disinfectants, and the concentration of aqueous chlorhexidine and chlorine-containing disinfectants needs to be increased compared with the current standards of the healthcare industry. It reminds us to strengthen the monitoring of disinfectant resistance to super drug-resistant bacteria.

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“…are opportunistic pathogens. Klebsiella pneumoniae is considered an opportunistic pathogen in humans, constituting an ongoing health concern for immunocompromised patients, the elderly, and neonates [ 27 ]. Over the past 30 years, interest in reptiles as pets has increased, and this interest can also be observed worldwide.…”

Section: Discussionmentioning

confidence: 99%

Klebsiella sp.-related infectious spondylitis in a bearded dragon (Pogona vitticeps)

Vetere¹,

Bertocchi

Pelizzone³

et al. 2021

BMC Vet Res

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Background Spondylitis is an inflammation of the vertebrae that leads to a destructive process with exuberant new bone formation. Osteomyelitis can produce a distortion of the bone architecture, degenerative joint changes and ankyloses of adjacent vertebrae. In reptiles, intervertebral discs are absent, so the term discospondylitis is not used. In lizards, vertebral lesions have not been well studied. The present paper describes the first case of Klebsiella sp.-related spondylitis in a pet lizard (Pogona vitticeps). Case presentation A 2-year-old, female bearded dragon (Pogona vitticeps) was presented for clinical examination due to a decreased activity level, decreased appetite and constipation. Blood tests showed no remarkable alterations. The haemogram showed normal parameters with relative lymphocytosis, although the absolute number of lymphocytes did not differ from the reference values. A computed tomography scan revealed a mixed osteolytic-proliferative bone lesion diffusing to the first and last tracts of the pre-sacral vertebrae together. A small amount of material obtained from the spinal swelling was sampled with an aseptic technique for bacterial culture, which was positive for Klebsiella sp. The antibiogram revealed sensitivity to enrofloxacin, marbofloxacin, and chloramphenicol and intermediate sensitivity to gentamicin. Complete return to spontaneous feeding was achieved 15 days after the beginning of antibiotic and anti-inflammatory therapy. Conclusions In veterinary medicine, spondylitis represents a well-known disease in small companion animals. In mammals, the most common aetiologic agents are fungi and bacteria. Antibiotic therapy was set based on the antibiogram, and marbofloxacin was chosen at a dosage of 10 mg/kg subcutaneously (SC) once per day (SID). After only 7 days of antibiotic therapy, the clinical condition improved significantly; the patient started feeding and drinking spontaneously and gained weight. This case should remind clinicians of the importance of always performing antibiograms before choosing any antibiotic therapy. Considering reptiles, there have been few papers about spinal diseases, mostly regarding snakes and a few about Iguana iguana. Relative to other species of saurians, the literature remains lacking.

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Klebsiella pneumoniae: Prevalence, Reservoirs, Antimicrobial Resistance, Pathogenicity, and Infection: A Hitherto Unrecognized Zoonotic Bacterium

Cited by 46 publications

References 270 publications

Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae

Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae

Disinfection Strategies of Carbapenem-resistant Klebsiella Pneumoniae in a Healthcare Facility

Klebsiella sp.-related infectious spondylitis in a bearded dragon (Pogona vitticeps)

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