In vivo typing of Escherichia coli obtained from laying chickens with the E. coli peritonitis syndrome

Landman, W. J. M.; Buter, G J; Dijkman, Remco; Eck, J. H. H. van

doi:10.1080/03079457.2021.1962004

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…In total, 1 ml (8 log 10 CFU/ml) cultivated bacteria per bird was administered via the intratracheal route. The concentration of the bacteria was selected according to previous studies ( 31 , 32 ). For birds in groups N and V, PBS was administered in the same way.…”

Section: Methodsmentioning

confidence: 99%

Aerosol vaccination of chicken pullets with irradiated avian pathogenic Escherichia coli induces a local immunostimulatory effect

Bagheri,

Mitra,

Paudel

et al. 2023

Front. Immunol.

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The present study investigated the expression of cytokines and cellular changes in chickens following vaccination with irradiated avian pathogenic Escherichia coli (APEC) and/or challenge. Four groups of 11-week-old pullets, each consisting of 16 birds were kept separately in isolators before they were sham inoculated (N), challenged only (C), vaccinated (V) or vaccinated and challenged (V+C). Vaccination was performed using irradiated APEC applied via aerosol. For challenge, the homologous strain was administered intratracheally. Birds were sacrificed on 3, 7, 14 and 21 days post challenge (dpc) to examine lesions, organ to body weight ratios and bacterial colonization. Lung and spleen were sampled for investigating gene expression of cytokines mediating inflammation by RT-qPCR and changes in the phenotype of subsets of mononuclear cells by flow cytometry. After re-stimulation of immune cells by co-cultivation with the pathogen, APEC-specific IFN-γ producing cells were determined. Challenged only birds showed more severe pathological and histopathological lesions, a higher probability of bacterial re-isolation and higher organ to body weight ratios compared to vaccinated and challenged birds. In the lung, an upregulation of IL-1β and IL-6 following vaccination and/or challenge at 3 dpc was observed, whereas in the spleen IL-1β was elevated. Changes were observed in macrophages and TCR-γδ+ cells within 7 dpc in spleen and lung of challenged birds. Furthermore, an increase of CD4+ cells in spleen and a rise of Bu-1+ cells in lung were present in vaccinated and challenged birds at 3 dpc. APEC re-stimulated lung and spleen mononuclear cells from only challenged pullets showed a significant increase of IFN-γ+CD8α+ and IFN-γ+TCR-γδ+ cells. Vaccinated and challenged chickens responded with a significant increase of IFN-γ+CD8α+ T cells in the lung and IFN-γ+TCR-γδ+ cells in the spleen. Re-stimulation of lung mononuclear cells from vaccinated birds resulted in a significant increase of both IFN-γ+CD8α+ and IFN-γ+TCR-γδ+ cells. In conclusion, vaccination with irradiated APEC caused enhanced pro-inflammatory response as well as the production of APEC-specific IFN-γ-producing γδ and CD8α T cells, which underlines the immunostimulatory effect of the vaccine in the lung. Hence, our study provides insights into the underlying immune mechanisms that account for the defense against APEC.

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Section: Methodsmentioning

confidence: 99%

Aerosol vaccination of chicken pullets with irradiated avian pathogenic Escherichia coli induces a local immunostimulatory effect

Bagheri,

Mitra,

Paudel

et al. 2023

Front. Immunol.

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“…Again, the poultry-related ST115 [ 58 ] could be identified but failed to “establish” in the sentinel population. Another finding was ST1276, which was also associated with poultry farming [ 82 , 84 , 85 , 86 ]. In addition, it has been reported that the carriage of CTX-M-55 plasmid in ESBL- E. coli might be associated with spread to humans via food [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany

et al. 2022

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Antimicrobial resistance (AMR) is a serious global health threat with extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales as the most critical ones. Studies on AMR in wild birds imply a possible dissemination function and indicate their potential role as sentinel animals. This study aimed to gain a deeper insight into the AMR burden of wild waterfowl by sampling semi-wild mallard ducks used as sentinels and to identify if AMR bacteria could be recommended to be added to the pathogens of public health risks to be screened for. In total, 376 cloacal and pooled fecal samples were collected from the sentinel plant over a period of two years. Samples were screened for ESBL-carrying E. coli and isolates found further analyzed using antimicrobial susceptibility testing and whole-genome sequencing. Over the sampling period, 4.26% (16/376) of the samples were positive for ESBL-producing E. coli. BlaCTX-M-1 and blaCTX-M-32 were the most abundant CTX-M types. Although none of the top global sequence types (ST) could be detected, poultry-derived ST115 and non-poultry-related STs were found and could be followed over time. The current study revealed low cases of ESBL-producing E. coli in semi-wild mallard ducks, which proves the suitability of sentinel surveillance for AMR detection in water-associated wildlife.

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“…Yolk peritonitis directly impacts the economic value of laying hens by reducing their egg-laying rate and significantly increasing the occurrence of egg deformities and soft shells ( Srinivasan et al, 2013 ; Landman and Van Eck, 2015 ). Clinical causes of yolk peritonitis in chickens are complex and are usually due to improper feeding management and infestation by various pathogenic microorganisms in the environment, such as Escherichia coli ( E. coli ) ( Landman et al, 2021 ). Previous studies have shown that 15.39% of reproductive tract abnormalities in commercial laying hens from 21 to 80 wk of age were associated with E. coli yolk peritonitis.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Escherichia coli pathogenicity and drug resistance in yolk peritonitis

Li,

Fang,

Chen

et al. 2024

Poultry Science

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In vivo typing of Escherichia coli obtained from laying chickens with the E. coli peritonitis syndrome

Cited by 7 publications

References 34 publications

Aerosol vaccination of chicken pullets with irradiated avian pathogenic Escherichia coli induces a local immunostimulatory effect

Aerosol vaccination of chicken pullets with irradiated avian pathogenic Escherichia coli induces a local immunostimulatory effect

Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany

Characterization of Escherichia coli pathogenicity and drug resistance in yolk peritonitis

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