In vivo
 transposition of
 Minos
 , a
 Drosophila
 mobile element, in mammalian tissues

Zagoraiou, Laskaro; Drabek, Dubravka; Alexaki, Sofia; Guy, Jacky; Klinakis, Apostolos; Langeveld, An; Skavdis, George; Mamalaki, Clio; Grosveld, Frank; Savakis, Charalambos

doi:10.1073/pnas.201392398

Cited by 68 publications

(56 citation statements)

References 28 publications

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“…In this situation, an effective approach to develop transposable-element-mediated technologies applicable to mammals would be to use elements from other sources. Elements so far reported to transpose in mammals include Tc1 and Tc3 of Caenorhabidtis elegans (Schouten et al 1998;Fischer et al 2001), Himar1 of Haematobia irritans (Zhang et al 1998), Minos of Drosophila hydei (Zagoraiou et al 2001) and Sleeping Beauty synthesized on the basis of sequence information for fish elements (Ivics et al 1997). The last two elements have already contributed to various developments, including transgenesis (Izsvak et al 2000;Yant et al 2000), gene tagging (Horie et al 2001;Dupuy et al 2002) and promoter trapping (Klinakis et al 2000).…”

Section: Introductionmentioning

confidence: 99%

The medaka fish Tol2 transposable element can undergo excision in human and mouse cells

et al. 2003

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Tol2 is an active DNA-based transposable element identified in the medaka fish, Oryzias latipes. Originating from a vertebrate and belonging to the hAT (hobo/Activator/Tam3) transposable element family, featuring a wide distribution among organisms, Tol2 would be expected to be active if introduced into mammals. We, therefore, examined if excision, one part of the transposition reaction, can occur in human and mouse culture cells. A Tol2 clone was introduced into cells and, after incubation, recovered. PCR and sequencing analysis provided evidence for precise and near precise excision in these cells. Tol2 can thus be expected to serve as a material for developing a gene transfer vector and other genetic tools applicable to mammals. It was also suggested that an intact Tol2 element could retain autonomy as a transposable element in mammalian cells.

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Section: Introductionmentioning

confidence: 99%

The medaka fish Tol2 transposable element can undergo excision in human and mouse cells

et al. 2003

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“…The Minos element is active in insect and mammalian cells in culture and leads to stable insertions into germ-line chromosomes of embryos of several insect species (Loukeris et al 1995a,b;Catteruccia et al 2000a,b;Klinakis et al 2000a;Shimizu et al 2000;Pavlopoulos et al 2004) and of ascidians (Sasakura et al 2003). It is also functional in somatic and germ cells of mice (Zagoraiou et al 2001;Drabek et al 2003). The wide range of host organisms that permit transposition of this element and the fact that transposition produces stable transformants with high efficiency (Kapetanaki et al 2002), allowing genome-wide mutagenesis in mammalian cells (Klinakis et al 2000b), suggests that it is a versatile tool for functional genomic analysis.…”

mentioning

confidence: 99%

Minos as a Genetic and Genomic Tool in Drosophila melanogaster

et al. 2005

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Much of the information about the function of D. melanogaster genes has come from P-element mutagenesis. The major drawback of the P element, however, is its strong bias for insertion into some genes (hotspots) and against insertion into others (coldspots). Within genes, 59-UTRs are preferential targets. For the successful completion of the Drosophila Genome Disruption Project, the use of transposon vectors other than P will be necessary. We examined here the suitability of the Minos element from Drosophila hydei as a tool for Drosophila genomics. Previous work has shown that Minos, a member of the Tc1/mariner family of transposable elements, is active in diverse organisms and cultured cells; it produces stable integrants in the germ line of several insect species, in the mouse, and in human cells. We generated and analyzed 96 Minos integrations into the Drosophila genome and devised an efficient ''jumpstarting'' scheme for production of single insertions. The ratio of insertions into genes vs. intergenic DNA is consistent with a random distribution. Within genes, there is a statistically significant preference for insertion into introns rather than into exons. About 30% of all insertions were in introns and 55% of insertions were into or next to genes that have so far not been hit by the P element. The insertion sites exhibit, in contrast to other transposons, little sequence requirement beyond the TA dinucleotide insertion target. We further demonstrate that induced remobilization of Minos insertions can delete nearby sequences. Our results suggest that Minos is a useful tool complementing the P element for insertional mutagenesis and genomic analysis in Drosophila. O NE of the main goals of modern genetics is to link the many thousands of genes identified through the sequencing of whole genomes of model organisms to gene function. The most powerful technique for this purpose so far has been transgenesis with mobile elements. This technique is a means to disrupt, overexpress, or misexpress single genes to identify expression patterns and also to characterize genetic pathways and their interactions. One of the main advantages of insertional mutagenesis over the classical method of chemical mutagenesis is the ease with which the targeted gene can be identified, since it carries an inserted tag.The P element was the first mobile element that enabled germ-line transformation of an insect species (Rubin and Spradling 1982). Since then, thousands of single P-element insertions causing lethality, semilethality, sterility, semisterility, and visible phenotypes have been created and analyzed in Drosophila (Cooley et al.

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“…Like other members of the Tc1/Mariner superfamily of transposons, Minos recognizes and integrates at a TA dinucleotide that is duplicated upon excision. Transgenic mice carrying the Minos transposase cDNA under the control of the human CD2 promoter mobilized a Minos transposon carrying a CMVGFP reporter gene in a CD2-specific manner (Zagoraiou et al, 2001). However, the efficiency of transposition was lower in mouse T cells than in HeLa cells and also lower than that reported for SB in mouse embryonic stem cells.…”

Section: Transposon-mediated Mutagenesis In Vertebratesmentioning

confidence: 90%

“…Another vertebrate transposon, Minos, has also been described recently (Klinakis et al, 2000;Zagoraiou et al, 2001;Drabek et al, 2003). Minos, like SB, is a member of the Tc1/Mariner transposon family.…”

Section: Transposon-mediated Mutagenesis In Vertebratesmentioning

confidence: 99%

Engineering mutations: Deconstructing the mouse gene by gene

Raymond¹,

Soriano²

2006

Developmental Dynamics

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Over the past years new vectors and methodologies have been developed to carry out large-scale genomewide insertional mutagenesis screens in the mouse. Gene trapping, the most commonly used technique, is based on the insertion of a retroviral-or plasmid-based vector into a gene, resulting in a loss-of-function mutation, while simultaneously reporting its expression pattern and providing a molecular tag to facilitate cloning. The discovery of vertebrate DNA transposons in the mouse and recent improvements has also led to their increased use in insertional mutagenesis screens. Several public resources have been set-up recently by the academic community to distribute information and materials generated from these largescale screens. These new resources should accelerate the study and understanding of biological and developmental processes. Developmental Dynamics 235:2424 -2436, 2006.

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In vivo transposition of Minos , a Drosophila mobile element, in mammalian tissues

Cited by 68 publications

References 28 publications

The medaka fish Tol2 transposable element can undergo excision in human and mouse cells

The medaka fish Tol2 transposable element can undergo excision in human and mouse cells

Minos as a Genetic and Genomic Tool in Drosophila melanogaster

Engineering mutations: Deconstructing the mouse gene by gene

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