<i>In vivo</i> study of alginate hydrogel conglutinating cells to polycaprolactone vascular scaffolds fabricated by electrospinning

Sun, Kwang-Hsiao; Liu, Zhao; Liu, Changjian; Yu, Tong; Zhou, Min; Liu, Cheng; Ran, Feng; Pan, Lijia; Zhang, Huan

doi:10.1002/jbm.b.33731

Cited by 8 publications

(4 citation statements)

References 48 publications

(116 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, both smooth muscle and fibroblast cells also show difficulties in adherence to the surface of the materials, which may slow the progression of tissue hyperplasia to some extent. Therefore, implantation of artificial blood vessels constructed with this material would prolong the long‐term patency rate within human vessels . When polyester woven fabrics were used as stent grafts for implantation within humans, the cells concentrated on both sides of the monofilament of the interlacing.…”

Section: Resultsmentioning

confidence: 99%

Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures

Shen

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: The perennial problems associated with long-term patency of small diameter vascular grafts have yet to be satisfactorily resolved. One approach to rectify this problem is a rapid endothelialization on the inner wall of the grafts, which could significantly reduce inflammation or hyperplasia. Two critical factors that can affect this process in vivo include that of the biocompatibility of the materials and adhesion strength of cells on its surfaces. Thus, an important area of investigation involves an evaluation of cell adhesion on biomaterials in a dynamic culture system, but only limited research has been directed at examining the relationship between the shear forces resulting from flow rates and cell adhesion on the surface of materials. In this study, an in vitro dynamic culture system was utilized for use in examining such relationship by changing the flow rates. RESULTS: Cells adherent on monofilaments were dislodged with a shear force of 5.4 dyn, while most cells adherent on multifilaments tolerated shear forces of 7.0 dyn. CONCLUSION: Priority rankings of materials could be generated as a function of the shear forces tested, and the design or application of artificial blood vessel materials for use within specific regions of the body could be optimized.

show abstract

Section: Resultsmentioning

confidence: 99%

Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures

Shen

et al. 2018

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…4), so gel erosion within the eye cavity could also contribute to the observed microstructure. SEM has similarly been used to analyse the morphology of chitosan hydrogels and hydrogel-coated PCL scaffolds before and after implantation and study the effects of in vivo interactions on the hydrogel [58,59]. Overall, the SEM images suggest that the gel is mostly still present within the vitreous, but with changes in microstructure observed.…”

Section: Analysis Of Gels Harvested From Vitreousmentioning

confidence: 99%

Injectable PTHF-based thermogelling polyurethane implants for long-term intraocular application

et al. 2022

View full text Add to dashboard Cite

Background Hydrogels show great potential to be used for intraocular applications due to their high-water content and similarity to the native vitreous. Injectable thermosensitive hydrogels through a small-bore needle can be used as a delivery system for drugs or a tamponading substitute to treat posterior eye diseases with clear clinical potential. However, none of the currently available thermosensitive hydrogels can provide intraocular support for up to 3 months or more. Method In this study, an injectable polytetrahydrofuran (PTHF)-based thermosensitive hydrogel was synthesized by polyurethane reaction. We examined the injectability, rheological properties, microstructure, cytotoxicity, and in vivo compatibility and stability of the hydrogels in rabbit eyes. Results We found that the PTHF block type and PTHF component ratio could modulate thermogelation properties of the polyurethane polymers. The PTHF-based hydrogel implants retained normal retinal structure and function. Incorporating bioinert PTHF generated highly biocompatible and more stable thermogels in the vitreous cavity, with gel networks and the presence of polymer still observed after 3 months when other thermogels would have been completely cleared. Moreover, despite lacking hydrolytically cleavable linkages, the polymers could be most naturally removed from the native vitreous by bio-erosion without additional surgical interventions. Conclusion Our findings suggest the potential of incorporating hydrophobic bioinert blocks to enhance the in vivo stability of supramolecularly associated hydrogels for long-term intraocular applications. Graphical Abstract

show abstract

“…PCL nano ber scaffolds have also been shown to guide and differentiate human mesenchymal stem cells into cartilage. Therefore, combining PCL with brin improves its characterization [9,10].…”

Section: Introductionmentioning

confidence: 99%

Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine

Honarvar,

Setayeshmehr,

Hashemibeni

et al. 2023

Preprint

View full text Add to dashboard Cite

Today, cartilage tissue engineering (CTE) is considered important due to the lack of repair of cartilaginous lesions and the absence of appropriate methods for treatment. In this study, polycaprolactone (PCL) scaffolds were fabricated by three-dimensional (3D) printing and were then coated with fibrin (F) and acellular solubilized extracellular matrix (ECM). After extracting adipose-derived stem cells (ADSCs), 3D-printed scaffolds were characterized and compared to hydrogel groups. After inducing the chondrogenic differentiation in the presence of Piascledine and comparing it with TGF-β3 for 28 days, the expression of genes involved in chondrogenesis (AGG, COLII) and the expression of the hypertrophic gene (COLX) were examined by real-time PCR. The expression of proteins COLII and COLX was also determined by immunohistochemistry. Glycosaminoglycan was measured by toluidine blue staining. 3D-printed scaffolds clearly improved cell proliferation, viability, water absorption, and compressive strength compared to the hydrogel groups. Moreover, the use of compounds such as ECM and Piascledine in the process of ADSCs chondrogenesis induction increased cartilage-specific markers and decreased the hypertrophic marker compared to TGF-β3. In Piascledine groups, the expression of COLL II protein, COLL II and Aggrecan genes, and the amount of glycosaminoglycan showed a statistically significant increase in the PCL/F/ECM compared to the PCL and PCL/F groups (P < 0.05). The results confirmed that the PCL/F/ECM scaffolds presented in this study afford unique opportunities for CTE.

show abstract

In vivo study of alginate hydrogel conglutinating cells to polycaprolactone vascular scaffolds fabricated by electrospinning

Abstract: AHCC are an effective method for seeding cells on vascular scaffolds and can eliminate the time delay for cell adhesion. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2443-2454, 2017.

Cited by 8 publications

References 48 publications

Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures

Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures

Injectable PTHF-based thermogelling polyurethane implants for long-term intraocular application

Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine

Contact Info

Product

Resources

About