<i>In vivo</i> stimulation and inhibition of granulopoiesis: the effect of an inflammatory reaction on murine diffusion chamber granulopoiesis

Symann, Michel; Anckaert, Ma.; Huybrechts, M.; Ninane, J.; Canon, Jl.; Sokal, G.

doi:10.1111/j.1365-2141.1982.tb07293.x

Cited by 10 publications

(7 citation statements)

References 18 publications

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“…This model allows one to study in vivo, by culture in diffusion chambers, the mechanisms of haematopoiesis in response to acute inflammation. Previous studies have shown that aseptic abscess formation is associated with an increase in the cellular content of diffusion chambers, including proliferative and non-proliferative granulocytes, macrophages, CFU-S and CFU-GM (Symann et al 1982). The present study demonstrates that cell proliferation in diffusion chambers depends on the importance of inflammation.…”

Section: Discussionsupporting

confidence: 71%

“…The findings elicited in diffusion chambers are relevant to the findings in the host haematopoietic system. Previous studies have shown that blood neutrophil counts, as well as numbers of marrow myeloid and progenitor cells are significantly increased in mice stimulated by an inflammation (Symann et al 1982, Hamood et al 1985. The effects on host haematopoietic cells are comparable to those on the diffusion chamber cells.…”

Section: Discussionmentioning

confidence: 98%

“…1978). A diffusion chamber culture system has been exploited to demonstrate that a humoral regulatory mechanism controls granulocyte production in acute inflammation (Symann et al 1982). The aim of the present study was to investigate whether humoral stimulating factors are produced quantitatively according to the degree of inflammation, and what cells secrete these diffusible factors.…”

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confidence: 99%

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In vivo regulation of granulocyte protluction in acute inflammation

Hamood

Fondu²

1991

Cell Proliferation

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In order to study in vivo the enhanced granulopoiesis that occurs during acute inflammation, 1-3 sterile metallic copper rods were inserted subcutaneously into mice either at the same place (one abscess) or at different sites (multiple abscesses). Diffusion chambers filled with bone marrow cells were implanted intraperitoneally for 3 days. When a single abscess was created, the granulocytic content of the diffusion chamber increased similarly whatever the number of inserted copper rods. However, there was a direct relationship between the number of abscesses and the number of granulocytic cells harvested from the diffusion chambers. In order to investigate the role of T-lymphocytes in the production of diffusible stimulating factors that act on diffusion chamber granulopoiesis, cyclosporin A (CyA) was given to the mice with implanted copper rods. CyA abrogated the induced enhancement of CFU-S, CFU-GM and mature granulocyte numbers inside the diffusion chamber. The stimulatory effect of inflammation on diffusion chamber granulopoiesis was not observed in T-lymphocyte-deficient nude mice. These data suggest that in vivo stimulation of granulopoiesis is related to the level of inflammation, and that this effect requires the functional integrity of T-lymphocytes.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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In vivo regulation of granulocyte protluction in acute inflammation

Hamood

Fondu²

1991

Cell Proliferation

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“…Finally, production of haematopoietic cytokines that we did not measure, could have been stimulated to a different degree by VLB and CY, thus explaining the lack of perfect correlation between the present cytokine and granulocytopoiesis results in mice given cytotoxic treatment. Other investigators have also stimulated haematopoiesis in diffusion chambers, with injections of lithium carbonate (25) or implantation of copper rods as in the present study (7), but without being able to measure raised levels of CSF in the chambers.…”

Section: Discussionmentioning

confidence: 84%

Granulocytopoiesis versus cytokine activities in peritoneal diffusion chamber cultures in the mouse

Wang

Brekken

Siebke

et al. 2000

European J of Haematology

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Regulation of granulocyte formation was studied by correlating granulocytopoiesis in diffusion chambers with activities of putative regulatory cytokines in such chambers. The implantation procedure increased the levels in 1- and 2-d chambers of interleukin 6 (IL-6), G-CSF, TNF-alpha, and non-specific granulocyte/macrophage (G/M) colony-stimulating activities (CSA), assessed with bioassays and immunoassays. The activities subsided rapidly thereafter. They could be increased by vinblastine, cyclophosphamide, and a sterile inflammatory reaction (s.c. implanted copper rods; Cu-r). Anti-inflammatory indomethacin curtailed the IL-6, but raised the TNF-alpha, G-CSF, and CSA levels in Cu-r mice. Interferon inducer poly-I:C augmented G-CSF, but decreased TNF-alpha levels. Mouse blood cells cultured in chambers expanded their granulocyte/macrophage progenitor population rapidly during the first week of culture; this population being significantly larger on day 3 in perturbed than in unperturbed mice. A marked decline followed in the second week, significantly larger in mice given cytotoxic treatment than in controls. Peritoneal G-CSF and TNF-alpha may explain progenitor and granulocyte growth and development in diffusion chambers during the first week of culture. GM-CSF and IL-3 were apparently without any influence. None of the peritoneal cytokines assayed could explain the population decline during the second week, which was possibly caused by exhaustion of earlier progenitor cells, rather than by intra-chamber feedback mechanisms.

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“…Pretreatment of host animals with either irradiation or chemotherapeutic agents causing cytopenia leads to enhanced cell production in intraperitoneally implanted DCs concomitant with hematopoietic cell recovery in the bone marrow and spleen [8,13,22,. Similar stimulation occurs following induction of an aseptic inflammation [92,931 or administration of lithium [94,951 or corticosteroids [96]. Activity produced by antigen-or Schistosomu munsoni-stimulated lymphoid cells [97,98] seems to be a good source of an eosinophilopoietic factor.…”

Section: Humoml Effectsmentioning

confidence: 93%

Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

Niskanen¹,

Chatelain²,

Symann³

1989

The International Journal of Cell Cloning

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Assay of hematopoietic precursor cells in diffusion chambers (DCs) implanted intraperitoneally in experimental animals provides a powerful tool for studying stem cell kinetics in vivo. In this system, the effect of cell migration (which complicates whole animal studies) is eliminated because the membranes utilized in the construction of the chambers are impermeable for cells, while permitting free passage of molecules present in the humoral phase of the host. As judged by light microscopy, conditions in the DC cultures primarily favor macrophage and granulocyte growth. However, the use of in vitro and in vivo subculture to further analyze chamber contents has demonstrated that the system supports proliferation of early hematopoietic progenitors. Additionally, cells capable of rescuing lethally irradiated mice proliferate in DC cultures. Development of the plasma clot DC technique has revealed that most of the growth occurs in colonies which are derived from single cells (CFU-d). Characterization of these cells indicates that they are at least as primitive as other colony-forming cells and, also based on subculture studies, can differentiate along several hematopoietic lineages. In addition to normal CFU-d, both embryonal and leukemic cells can give rise to granulocytes, macrophages, megakaryocytes and erythroid cells in the DC cultures. Evaluation of the effects of humoral factors on hematopoietic cell proliferation and differentiation in the system has led to the identification of both stimulators and inhibitors that may be different from the well-characterized cytokines. Thus, the system seems to be useful for detecting molecules controlling the most primitive stages of hematopoiesis. We believe that the DC culture technique holds enormous potential in the study of stem cell proliferation and differentiation in vivo.

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In vivo stimulation and inhibition of granulopoiesis: the effect of an inflammatory reaction on murine diffusion chamber granulopoiesis

Cited by 10 publications

References 18 publications

In vivo regulation of granulocyte protluction in acute inflammation

In vivo regulation of granulocyte protluction in acute inflammation

Granulocytopoiesis versus cytokine activities in peritoneal diffusion chamber cultures in the mouse

Diffusion chamber colony‐forming unit (cfu‐d): A primitive stem cell

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