<i>In vivo</i> Preservation of the Hybrid Layer by Chlorhexidine

Carrilho, Marcela Rocha de Oliveira; Geraldeli, Saulo; Tay, Franklin R.; Góes, Mário Fernando de; Carvalho, Ricardo Marins de; Tjäderhane, Leo; Reis, André Figueiredo; Hebling, Josimeri; Mazzoni, Annalisa; Breschi, Lorenzo; Pashley, David H.

doi:10.1177/154405910708600608

Cited by 500 publications

(668 citation statements)

References 25 publications

(38 reference statements)

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“…Previous studies have demonstrated that the use of chlorhexidine as an MMP inhibitor preserves the integrity of the hybrid layers created by commercial adhesives (Hebling et al, 2005;Carrilho et al, 2007b;Breschi et al, 2009). Nevertheless, increases in adhesive joint failures were observed in aged specimens pre-treated with chlorhexidine (Zhou et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Ethanol wet-bonding differs conceptually from the use of chlorhexidine, a potent MMP inhibitor (Gendron et al, 1999), in preventing hybrid layer degradation (Pashley et al, 2004;Hebling et al, 2005;Carrilho et al, 2007aCarrilho et al, , 2007bBreschi et al, 2009). In theory, degradation of the interface should not occur when water within the intrafibrillar and interfibrillar compartments of a collagen matrix is completely replaced by resin.…”

mentioning

confidence: 99%

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Ethanol Wet-bonding Challenges Current Anti-degradation Strategy

et al. 2010

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The long-term effectiveness of chlorhexidine as a matrix metalloproteinase (MMP) inhibitor may be compromised when water is incompletely removed during dentin bonding. This study challenged this anti-bond degradation strategy by testing the null hypothesis that wet-bonding with water or ethanol has no effect on the effectiveness of chlorhexidine in preventing hybrid layer degradation over an 18-month period. Acid-etched dentin was bonded under pulpal pressure simulation with Scotchbond MP and Single Bond 2, with water wet-bonding or with a hydrophobic adhesive with ethanol wetbonding, with or without pre-treatment with chlorhexidine diacetate (CHD). Resin-dentin beams were prepared for bond strength and TEM evaluation after 24 hrs and after aging in artificial saliva for 9 and 18 mos. Bonds made to ethanol-saturated dentin did not change over time with preservation of hybrid layer integrity. Bonds made to CHD pretreated acid-etched dentin with commercial adhesives with water wet-bonding were preserved after 9 mos but not after 18 mos, with severe hybrid layer degradation. The results led to rejection of the null hypothesis and highlight the concept of biomimetic water replacement from the collagen intrafibrillar compartments as the ultimate goal in extending the longevity of resin-dentin bonds.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Ethanol Wet-bonding Challenges Current Anti-degradation Strategy

et al. 2010

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“…Chlorhexidine (CHX) is a broad-spectrum cationic antimicrobial agent and has been studied as a protease inhibitor in adhesive dentistry to preserve the integrity of collagen matrices in the hybrid layer (33,120,121). In the absence of bacterial and salivary protease, residual collagenolytic activity was found in partially demineralized dentin powder from extracted, caries free teeth (65,66,122,123).…”

Section: Chlorhexidinementioning

confidence: 99%

“…CHX has been shown to inhibit MMP-2, -8, and -9 activities at low concentrations (124). The collagenase activity is inhibited as long as CHX remains bound to the collagen matrix and CHX-treated bonded dentin was shown to be stable over 14 months (121). To improve the water solubility of CHX, it is used as a salt by the addition of an acid.…”

Section: Chlorhexidinementioning

confidence: 99%

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Persadmehr

Torneck

Cvitkovitch

et al. 2014

Journal of Endodontics

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This study evaluated the ability of photodynamic therapy (PDT), chitosan nanoparticles (CSnp), or their combination, to inhibit bacterial collagenase-mediated degradation of collagen. Rat type 1 fibrillar collagen matrices were untreated or treated with 2.5% glutaraldehyde (GD), 2.5% GD followed by 1% CSnp, 1% CSnp, PDT, or 1% CSnp followed by PDT. Samples, except untreated controls, were exposed to Clostridium histolyticum collagenase. The soluble digestion products were assessed by hydroxyproline assay and the remaining adherent collagen was quantified by picrosirius red (PSR) staining. Collagen treated with CSnp, PDT, or a combination of CSnp and PDT, exhibited less degradation than controls. The abundance of post-treatment residual collagen correlated with the extent of degradation. Fourier transform infrared (FTIR) spectroscopy analysis showed that PDT treatment enhanced collagen cross-linking.Immunoblotting of sedimented CSnp indicated that CSnp and collagenase bound with low affinity. However, CSnp-bound collagenase showed a significant reduction in collagenolytic activity compared with controls.III

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“…Studies have shown that matrix metalloproteinase (MMP) inhibitors, such as chlorhexidine (CHX) and more recently sodium fluoride (NaF), are able to preserve the structural integrity of the hybrid layer and consequently improve the longevity of restorations (11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Effect of Metalloproteinase Inhibitors on the Microtensile Bond Strength of Composite Resin to Er:YAG Laser-Irradiated Dentin

et al. 2016

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This study evaluated the effect of matrix metalloproteinase (MMP) inhibitors -2% (CHX) and sodium fluoride (NaF) (5000 ppm) -on microtensile bond strength (μTBS) of composite resin to Er:YAG laser-irradiated dentin after chemical degradation of the bond interface. The occlusal surface of forty sound human molars was removed exposing the dentin surface (n=10), which was polished, irradiated with Er:YAG laser, acid etched and dried. Twenty specimens were rewetted with 2% CHX (control group) and 20 were rewetted with NaF (5000 ppm). The adhesive system was applied and a 4-mm-high plateau of light-cured composite resin was built up. Resin-dentin sticks were obtained with a rectangular cross-sectional area (0.8-1 mm 2 ) and were either stored in water at 37 °C for 24 h or submitted to chemical degradation. For chemical degradation, they were immersed in 10% NaOCl aqueous solution for 5 h and rinsed in water for 1 h. The sticks were submitted to microtensile test in a mechanical testing machine at 0.5 mm/min until failure. Fracture pattern was analyzed using SEM. μTBS values were calculated in MPa and submitted to analysis of variance ANOVA (α=0.05). The variance analysis showed that the 'MMP inhibitor ' and 'degradation' factors (p=0.214 and p=0.093, respectively) and interaction between the factors were not statistically significant (p=0.143). Mixed failure predominated in all groups. In conclusion, the 2% CHX and NaF 5000 ppm presented similar μTBS of composite resin to laser-irradiated dentin before and after chemical degradation

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In vivo Preservation of the Hybrid Layer by Chlorhexidine

Cited by 500 publications

References 25 publications

Ethanol Wet-bonding Challenges Current Anti-degradation Strategy

Ethanol Wet-bonding Challenges Current Anti-degradation Strategy

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Effect of Metalloproteinase Inhibitors on the Microtensile Bond Strength of Composite Resin to Er:YAG Laser-Irradiated Dentin

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