In vivooptimized three-photon imaging of intact mouse tibia links plasma cell motility to functional states in the bone marrow
Asylkhan Rakhymzhan,
Alexander F. Fiedler,
Robert Günther
et al.
Abstract:Intravital multi-photon imaging of the bone marrow is crucial to the study of cellular dynamics, communication with the microenvironment and functions, however, imaging of deep tissue areas is challenging and minimally invasive methods for deep-marrow imaging in intact long bones are needed. We developed a high pulse energy 1650 nm laser prototype, which permits to surpass >100 µm thick cortical bone and to perform three-photon microscopy (3PM) in more than 400 µm depth in the marrow cavity of intact mouse … Show more
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