<i>In vivo</i> noninvasive measurement of skin autofluorescence biomarkers relate to cardiovascular disease in mice

Akbar, Naveed; Sokolovski, Sergei G.; Dunaev, Andrey V.; Belch, J. J. F.; Rafailov, Edik U.; Khan, Faisel

doi:10.1111/jmi.12135

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…Sample spectra were exported as ASCII files and combined, plotted and visualised using Origin Pro 8 analysis software. From the custom‐made LDF3.1 software, amplitudes of fluorescence for specific fluorophores of interest were recorded, in keeping with previous work by Dunaev et al , Akbar et al and Smirnova et al . The excitation and emission wavelengths for the endogenous fluorophores of interest were as follows (Table ).…”

Section: Methodsmentioning

confidence: 99%

Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

et al. 2016

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Bladder cancer is among the most common cancers in the UK and conventional detection techniques suffer from low sensitivity, low specificity, or both. Recent attempts to address the disparity have led to progress in the field of autofluorescence as a means to diagnose the disease with high efficiency, however there is still a lot not known about autofluorescence profiles in the disease. The multi‐functional diagnostic system “LAKK‐M” was used to assess autofluorescence profiles of healthy and cancerous bladder tissue to identify novel biomarkers of the disease. Statistically significant differences were observed in the optical redox ratio (a measure of tissue metabolic activity), the amplitude of endogenous porphyrins and the NADH/porphyrin ratio between tissue types. These findings could advance understanding of bladder cancer and aid in the development of new techniques for detection and surveillance.

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Section: Methodsmentioning

confidence: 99%

Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

et al. 2016

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“…It has been widely used in medical field for the detection of neoplastic growth in epithelial tissues, diagnosis of mucosal abnormalities and monitoring of various skin diseases including wound healing . Potential of this technique to monitor collagen during wound healing in preclinical models is well documented . Collagen is a major protein component of the extracellular matrix (ECM) responsible for providing normal skin with tensile strength, integrity and structure .…”

Section: Introductionmentioning

confidence: 99%

Probing endogenous collagen by laser‐induced autofluorescence in burn wound biopsies: A pilot study

Prabhu

Acharya

Rao

et al. 2018

Journal of Biophotonics

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The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.

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“…At the same time, antioxidant therapy with succinic acid 4 is capable of providing a neuroprotective effect in the development of neurodegenerative diseases. Succinic acid is an endogenous intracellular metabolite of the Krebs cycle 13 . Wh 0-520 nm 14 …”

Section: Introductionmentioning

confidence: 99%

Use of fluorescent optical fibre probe for recording parameters of brain metabolism in rat model

Stelmashchuk

Seryogina

Piavchenko

et al. 2018

Biophotonics: Photonic Solutions for Better Health Care VI

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This studiy was carried out on groups of clinically healthy male Wistar rats. Animals received distilled drinking water ad libitum for 1 month, water containing succinic acid, water containing zinc sulphate and succinate zinc. Using the method of fluorescence spectroscopy, the parameters of brain metabolism in vivo in a model of laboratory rats was investigated. Based on data obtained by fluorescence spectroscopy, we have registered a change in the degree of cellular respiration in different structures of the cerebral cortex with the toxic effect of zinc compounds and succinic acid on the oxygen exchange process.Keywords: zinc compounds, succinic acid, zinc sulphate, brain metabolism, fluorescence spectroscopy, blood microcirculation, optical measurements in vivo, neurodegenerative diseases *Olya.zh93@gmail.com; phone 7 919 269 79 92; www.bmecenter.ru/en/vascular-control

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In vivo noninvasive measurement of skin autofluorescence biomarkers relate to cardiovascular disease in mice

Abstract: SummaryBackground and objective

Cited by 15 publications

References 36 publications

Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

Optical redox ratio and endogenous porphyrins in the detection of urinary bladder cancer: A patient biopsy analysis

Probing endogenous collagen by laser‐induced autofluorescence in burn wound biopsies: A pilot study

Use of fluorescent optical fibre probe for recording parameters of brain metabolism in rat model

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