<i>In Vivo</i>Non Invasive Molecular Imaging for Immune Cell Tracking in Small Animals

Youn, Hyewon; Hong, Kee-Jong

doi:10.4110/in.2012.12.6.223

Cited by 32 publications

(36 citation statements)

References 22 publications

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“…Various modalities, such as bioluminescence imaging (BLI), nuclear, fluorescence, and magnetic resonance imaging (MRI) [63][64][65], have been used for in vivo imaging ( Table 2). In general, BLI is known to have the highest sensitivity and high signal-to-noise ratio while nuclear imaging has the highest penetration [63].…”

Section: Bioimaging Modalitiesmentioning

confidence: 99%

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Yi¹,

Lee²,

Kim

et al. 2020

IJMS

141

131

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Exosomes are nano-sized membranous vesicles produced by nearly all types of cells. Since exosome-like vesicles are produced in an evolutionarily conserved manner for information and function transfer from the originating cells to recipient cells, an increasing number of studies have focused on their application as therapeutic agents, drug delivery vehicles, and diagnostic targets. Analysis of the in vivo distribution of exosomes is a prerequisite for the development of exosome-based therapeutics and drug delivery vehicles with accurate prediction of therapeutic dose and potential side effects. Various attempts to evaluate the biodistribution of exosomes obtained from different sources have been reported. In this review, we examined the current trends and the advantages and disadvantages of the methods used to determine the biodistribution of exosomes by molecular imaging. We also reviewed 29 publications to compare the methods employed to isolate, analyze, and label exosomes as well as to determine the biodistribution of labeled exosomes.

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Section: Bioimaging Modalitiesmentioning

confidence: 99%

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Yi¹,

Lee²,

Kim

et al. 2020

IJMS

141

131

View full text Add to dashboard Cite

show abstract

“…SPIONs, fluorescent dyes, or radionuclides can be used as probes to directly prelabel stem cells for noninvasive tracking. 9,[21][22][23] Standardized protocols used for labeling stem cells with SPIONs were previously compiled by us, 15,24 and other direct-labeling reagents were reviewed by Marks and Nolan 25 and Progatzky et al 26 Indirect labeling is a considerably different method, which includes genetic modification in order to either produce an appropriate signal-generating molecule or increase the affinity of cells to contrast agents. 9,21,[27][28][29][30][31][32] Transient expression of reporter proteins by DNA vector transfection is often included in this set of cell labeling.…”

Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

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Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

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“…Indeed, fluorescent proteins' expression guarantees non-toxic, steady, and homogeneous labeling, fluorescence transmission to daughter cells, and its dynamic loss at death by physiological protein degradation. As such, this option most probably warrants a proper tracking of grafted cells and an accurate estimation of tumor volumes and dispersion across different time points [33][34][35].…”

Section: Choice Of a Suitable Cell Labeling Methodsmentioning

confidence: 99%

ZeOncoTest: Refining and Automating the Zebrafish Xenograft Model for Drug Discovery in Cancer

et al. 2019

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The xenograft of human cancer cells in model animals is a powerful tool for understanding tumor progression and metastatic potential. Mice represent a validated host, but their use is limited by the elevated experimental costs and low throughput. To overcome these restrictions, zebrafish larvae might represent a valuable alternative. Their small size and transparency allow the tracking of transplanted cells. Therefore, tumor growth and early steps of metastasis, which are difficult to evaluate in mice, can be addressed. In spite of its advantages, the use of this model has been hindered by lack of experimental homogeneity and validation. Considering these facts, the aim of our work was to standardize, automate, and validate a zebrafish larvae xenograft assay with increased translatability and higher drug screening throughput. The ZeOncoTest reliability is based on the optimization of different experimental parameters, such as cell labeling, injection site, automated individual sample image acquisition, and analysis. This workflow implementation finally allows a higher precision and experimental throughput increase, when compared to previous reports. The approach was validated with the breast cancer cell line MDA-MB-231, the colorectal cancer cells HCT116, and the prostate cancer cells PC3; and known drugs, respectively RKI-1447, Docetaxel, and Mitoxantrone. The results recapitulate growth and invasion for all tested tumor cells, along with expected efficacy of the compounds. Finally, the methodology has proven useful for understanding specific drugs mode of action. The insights gained bring a step further for zebrafish larvae xenografts to enter the regulated preclinical drug discovery path.

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In VivoNon Invasive Molecular Imaging for Immune Cell Tracking in Small Animals

Cited by 32 publications

References 22 publications

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Advances in Analysis of Biodistribution of Exosomes by Molecular Imaging

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

ZeOncoTest: Refining and Automating the Zebrafish Xenograft Model for Drug Discovery in Cancer

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