<i>In vivo</i> NMR spectroscopy: toward real time monitoring of environmental stress

Soong, Ronald; Nagato, Edward G.; Sutrisno, Andre; Fortier-McGill, Blythe; Akhter, Mohammad; Schmidt, Sebastian; Heumann, Hermann; Simpson, André J.

doi:10.1002/mrc.4154

Cited by 49 publications

(166 citation statements)

References 23 publications

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“…The ability to conduct in vivo NMR experiments hinges on a flow cell system that is capable of supplying food and oxygenated water to the organisms under investigation . The use of a flow cell system in NMR spectroscopy has been well documented .…”

Section: Flow Cell Designmentioning

confidence: 99%

“…A two pump system can be problematic because the flow rate of both pumps must be matched to avoid formation of air bubbles. Early work by Pincetich et al involved studying Oryzia latipes (Japanese rice fish) using a single recirculating pump design that was recently modified for both in vivo research and reaction monitoring . Flow cell design is one of the most important starting points for in vivo studies.…”

Section: Flow Cell Designmentioning

confidence: 99%

“…NMR spectroscopy is becoming a powerful tool for environmental and biomedical research . NMR is the only nondestructive technique capable of providing rich molecular information on even the most disordered and complex systems .…”

Section: Introductionmentioning

confidence: 99%

“…For clarity, in vivo NMR is defined here as small living organisms completely sustained within a flow system inside a vertical bore high‐resolution NMR spectrometer (as opposed to the traditional horizontal MRI systems). Flow‐based in vivo NMR offers several key advantages: (a) in vivo NMR can provide real‐time monitoring of metabolic flux, allowing scientists to identify biomarkers related to the exposure of environmental toxins at sub lethal concentrations. (b) In vivo NMR studies reduce natural variability as both control and exposed groups consist of the same individuals which can improve stress discrimination .…”

Section: Introductionmentioning

confidence: 99%

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Flow‐based in vivo NMR spectroscopy of small aquatic organisms

Soong

Mobarhan

Tabatabaei

et al. 2019

Magnetic Reson in Chemistry

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NMR applied to living organisms is arguably the ultimate tool for understanding environmental stress responses and can provide desperately needed information on toxic mechanisms, synergistic effects, sublethal impacts, recovery, and biotransformation of xenobiotics. To perform in vivo NMR spectroscopy, a flow cell system is required to deliver oxygen and food to the organisms while maintaining optimal line shape for NMR spectroscopy. In this tutorial, two such flow cell systems and their constructions are discussed: (a) a single pump high‐volume flow cell design is simple to build and ideal for organisms that do not require feeding (i.e., eggs) and (b) a more advanced low‐volume double pump flow cell design that permits feeding, maintains optimal water height for water suppression, improves locking and shimming, and uses only a small recirculating volume, thus reducing the amount of xenobiotic required for testing. In addition, key experimental aspects including isotopic enrichment, water suppression, and 2D experiments for both 13C enriched and natural abundance organisms are discussed.

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Section: Flow Cell Designmentioning

confidence: 99%

Section: Flow Cell Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Flow‐based in vivo NMR spectroscopy of small aquatic organisms

Soong

Mobarhan

Tabatabaei

et al. 2019

Magnetic Reson in Chemistry

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“…In high‐field NMR systems, there are two main approaches: (a) flow‐based NMR using solution state NMR probes and (b) magic angle spinning (MAS) based approaches. Flow‐based approaches provide a low stress environment, in which the organisms can be maintained indefinitely and is thus ideal for looking at metabolic responses to external stimuli. A separate tutorial in this special issue covers applications of flow‐based NMR, and therefore, it will not be discussed further here.…”

Section: Introductionmentioning

confidence: 99%

In vivo comprehensive multiphase NMR

Mobarhan

Soong

Lane

et al. 2019

Magnetic Reson in Chemistry

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Traditionally, due to different hardware requirements, nuclear magnetic resonance (NMR) has developed as two separate fields: one dealing with solids, and one with solutions. Comprehensive multiphase (CMP) NMR combines all electronics and hardware (magic angle spinning [MAS], gradients, high power Radio Frequency (RF) handling, lock, susceptibility matching) into a universal probe that permits a comprehensive study of all phases (i.e., liquid, gel‐like, semisolid, and solid), in intact samples. When applied in vivo, it provides unique insight into the wide array of bonds in a living system from the most mobile liquids (blood, fluids) through gels (muscle, tissues) to the most rigid (exoskeleton, shell). In this tutorial, the practical aspects of in vivo CMP NMR are discussed including: handling the organisms, rotor preparation, sample spinning, water suppression, editing experiments, and finishes with a brief look at the potential of other heteronuclei (2H, 15N, 19F, 31P) for in vivo research. The tutorial is aimed as a general resource for researchers interested in developing and applying MAS‐based approaches to living organisms. Although the focus here is CMP NMR, many of the approaches can be adapted (or directly applied) using conventional high‐resolution magic angle spinning, and in some cases, even standard solid‐state NMR probes.

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NMR and Metabolomics

Chasapi

Karagkouni

Matzarapi

et al. 2019

Encyclopedia of Life Sciences

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Metabolomics is one of the most attractive whilst still developing field of omics sciences, studying metabolites and the alterations of their levels. As an approach, metabolomics has the potential to identify using high‐throughput analytical precision statistically significant alterations, covering a broad spectrum of metabolic processes. What metabolomics can offer are qualitative and quantitative information, incorporating the consideration of more variables in analytical procedures. Measuring of metabolites and metabolic profiling provide an instant ‘snapshot’ which permits the follow up of the dynamics of complex biological systems and their mechanisms under physiological or abnormal conditions. Nuclear magnetic resonance (NMR) provides an excellent technique for profiling the biological fluids and is especially adept at characterising complex solutions. Advances in biochemical data obtained from NMR spectra allow us to observe the metabolome in a very accurate manner and thus estimate the complex index of biochemical processes, determining the health status of an organism. To date, metabolomics profiling is a powerful approach for examining disease‐related metabolic changes and is highly effective in the identification of new biomarkers. Key Concepts NMR is a novel, noninvasive, fast, accurate and reproducible bioanalytical method for metabolites identification and quantification in biological samples. Simple 1 H 1D NMR spectra are exploited for the definition of the physiological healthy status of organism's metabolism, based on a specific spectral pattern. NMR experiments selection depends on the biological fluid of interest. Metabolites identification and quantification and their role in a metabolomic experiment. The advantages of metabolic profiling and its utility in a potential diagnostic model. Monitoring of an individual's metabolic responses through the examination of its spectral fingerprint along specific time points. Significant alterations in metabolites concentrations are meaningful to exact interactions of pathways, perturbations and metabolite–metabolite correlations. Computational tools and global repositories in service of metabolomic data. Multivariate analysis techniques and their implementation in the field of NMR metabolomics. NMR metabolomics in clinical research, the intervention of the field and the impact in accessing unknown biological phenomena.

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In vivo NMR spectroscopy: toward real time monitoring of environmental stress

Cited by 49 publications

References 23 publications

Flow‐based in vivo NMR spectroscopy of small aquatic organisms

Flow‐based in vivo NMR spectroscopy of small aquatic organisms

In vivo comprehensive multiphase NMR

NMR and Metabolomics

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