<i>In vivo</i> MR imaging of magnetically labeled mesenchymal stem cells transplanted into rat liver through hepatic arterial injection

Cai, Jinhua; Zhang, Xuemei; Wang, Xin; Li, Chuan; Liu, Guan-xin

doi:10.1002/cmmi.231

Cited by 33 publications

(29 citation statements)

References 31 publications

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“…All MRI experiments were performed with a Magnetom Vision 1.5 T (Siemens, Germany). Typical imaging parameters for in vivo imaging of cerebral anatomy used a multiple spin-echo sequence and T2 weighted sequences, which have been previously used for detection of SPIO-labeled cells [6,12,19], to assess MRI sensitivity of MIRB-labeled cMSCs.…”

Section: Mrimentioning

confidence: 99%

“…Currently, various magnetic nanoparticles, such as superparamagnetic iron oxide (SPIO), have been developed for diverse functional analysis in biomedical research [10,11]. An appropriate magnetic nanoparticle must be nontoxic, biocompatible, efficient for intracellular labeling, and highly sensitive to detection such as Feridex, an FDA approved SPIO contrast agent [12,13]. However, a current barrier is limited MRI sensitivity, which cannot distinguish regenerative and differentiated cells from exogenous and endogenous transplanted cells.…”

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confidence: 99%

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Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging

Ren

Wang

Zou

et al. 2011

Sci. China Life Sci.

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Recently, transplantation of allogeneic and autologous cells has been used for regenerative medicine. A critical issue is monitoring migration and homing of transplanted cells, as well as engraftment efficiency and functional capability in vivo. Monitoring of superparamagnetic iron oxide (SPIO) particles by magnetic resonance imaging (MRI) has been used in animal models and clinical settings to track labeled cells. A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo. Bone marrow mesenchymal stem cells (MSCs) have been extensively investigated for their various therapeutic properties, and exhibit the potential to differentiate into cells of diverse lineages. In this study, cynomolgus monkey MSCs (cMSCs) were labeled with Molday ION Rhodamine-B™ (MIRB), a new SPIO agent, to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo. The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging. Our method is helpful for detection of transplanted stem cells in vivo, which is required for understanding mechanisms of cell therapy.

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Section: Mrimentioning

confidence: 99%

mentioning

confidence: 99%

Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging

Ren

Wang

Zou

et al. 2011

Sci. China Life Sci.

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“…Cai et al evaluated in vivo MRI for tracking the SPIO labeled MSCs transplanted into rat liver through hepatic arterial infusion. The labeled stem cells in liver could be detected and monitored in vivo with a 1.5 T clinical MR scanner for up to 7 days after transplantation [35]. Ju et al tracked intrasplenically transplanted MSCs labeled with SPIO by using MRI in vivo in a liver damaged rat model.…”

Section: Imaging Methods To Monitor Transplanted Stem Cells In Animentioning

confidence: 99%

Molecular Imaging of Stem Cell Transplantation for Liver Diseases: Monitoring, Clinical Translation, and Theranostics

Wang

Petrella

Nicosia

et al. 2016

Stem Cells International

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Stem cell transplantation has been investigated to rescue experimental liver failure and is promising to offer an alternative therapy to liver transplantation for liver diseases treatment. Several clinical studies in this field have been carried out, but the therapeutic benefit of this treatment is still controversial. A major obstacle to developing stem cell therapies in clinic is being able to visualize the cells in vivo. Imaging modalities allow optimization of delivery, detecting cell survival and functionality by in vivo monitoring these transplanted graft cells. Moreover, theranostic imaging is a brand new field that utilizes nanometer-scale materials to glean diagnostic insight for simultaneous treatment, which is very promising to improve stem cell-based therapy for treatment of liver diseases. The aim of this review was to summarize the various imaging tools that have been explored with advanced molecular imaging probes. We also outline some recent progress of preclinical and clinical studies of liver stem cells transplantation. Finally, we discuss theranostic imaging for stem cells transplantation for liver dysfunction and future opportunities afforded by theranostic imaging.

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“…With respect to the transplant field, nanoparticle approaches for imaging have predominately been used to monitor transplanted grafts [135, 136], track distribution (dispersion) of administered stem cells [137–139], gauge viability of implanted cells within scaffolds [140, 141] or within tissues [142, 143], and to evaluate drug release from scaffolds [144]. In summary, nanotechnology has the potential to provide powerful solutions to permit noninvasive imaging of organs and tissues before and after transplantation and as means to visualize the vasculature and enhance the resolution for superior medical imaging.…”

Section: Nanotechnology As a Tool In Transplant Therapymentioning

confidence: 99%

The Emerging Role of Nanotechnology in Cell and Organ Transplantation

Tasciotti

Cabrera²,

Evangelopoulos³

et al. 2016

Transplantation

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Transplantation is often the only choice many patients have when suffering from end stage organ failure. Although the quality of life improves after transplantation, challenges such as organ shortages, necessary immunosuppression with associated complications and chronic graft rejection limits its wide clinical application. Nanotechnology has emerged in the past two decades as a field with the potential to satisfy clinical needs in the area of targeted and sustained drug delivery, non-invasive imaging, and tissue engineering. In this paper, we provide an overview of popular nanotechnologies and a summary of the current and potential uses of nanotechnology in cell and organ transplantation.

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In vivo MR imaging of magnetically labeled mesenchymal stem cells transplanted into rat liver through hepatic arterial injection

Abstract: The magnetically labeled MSCs transplanted into rat liver through hepatic arterial injection can be detected and monitored in vivo with a 1.5 T clinical MR scanner for up to 7 days after cell transplantation.

Cited by 33 publications

References 31 publications

Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging

Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging

Molecular Imaging of Stem Cell Transplantation for Liver Diseases: Monitoring, Clinical Translation, and Theranostics

The Emerging Role of Nanotechnology in Cell and Organ Transplantation

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