In vivo monitoring of rat macrophages labeled with poly(l‐lysine)‐iron oxide nanoparticles

Abstract

Coprecipitation of FeCl2 and FeCl3 with aqueous ammonia was used to prepare iron oxide nanoparticles dispersible in aqueous medium. Oxidation of the particles with sodium hypochlorite then yielded maghemite (γ-Fe2 O3 ) nanoparticles which were coated with two types of coating -d-mannose or poly(l-lysine) (PLL) as confirmed by FTIR analysis. The particles were <10 nm according to transmission electron microscopy. Their hydrodynamic particle size was ∼180 nm (by dynamic light scattering). The d-mannose-, PLL-coated, and neat γ-Fe2 O3 particles as well as commercial Resovist® were used to label rat macrophages. The viability and contrast properties of labeled macrophages were compared. PLL-coated γ-Fe2 O3 nanoparticles were found optimal. The labeled macrophages were injected to rats monitored in vivo by magnetic resonance imaging up to 48 h. Transport of macrophages labeled with PLL-γ-Fe2 O3 nanoparticles in rats was confirmed. Tracking of macrophages using the developed particles can be used for monitoring of inflammations and cell migration in cell therapy.

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

“…NSCs have a great potency to regenerate the central nervous system, and are often used as cells of choice in brain applications [2–3]. Despite a prior positive experience of using PLL-γ-Fe 2 O 3 nanoparticles for cell labeling [22], the detailed analysis on biocompatibility of PLL-γ-Fe 2 O 3 nanoparticles was not described, neither the method of nanoparticle cell uptake defined.…”

Interaction of poly-L-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells

“…7 Various chemicals have been used to enhance cellular uptake of NPs, including poly-l-lysine (PLL), which is also applied to gene delivery [8][9][10] and cell labeling. [11][12][13][14] The addition of PLL to cultures enhances NP internalization by stem cells 15,16 and tumor cells. 16,17 PLL coating of NPs undergoes similar uptake enhancement in a variety of cells.…”

“…Our results indicated that both free and immobilized PLL greatly enhanced MNP uptake by glioma cells, which is consistent with previous findings that PLL enhanced NP internalization by stem cells 13,[15][16][17]19 and other cells. 11,14,[16][17][18]21 TEM results demonstrated microvilli formation associated with MNP internalization, suggesting that endocytosis may involve macropinocytosis, 20 which is usually the internalization pathway of particles .200 nm. 46 In our study, the number of internalized PLL-MNPs was much lower than Dex-MNPs with PLL in the culture medium, which was probably due to the smaller quantity of immobilized PLL.…”

Highly efficient magnetic hyperthermia ablation of tumors using injectable polymethylmethacrylate–Fe3O4

“…11,12 The magnetic media is the key factor for magnetic hyperthermia, which includes magnetic nanoparticles, magnetic uid and ferromagnetic thermoseeds. 13,14 For magnetic uid or magnetic nanoparticles, it is difficult to selectively accumulate in tumor tissue 15,16 and can easily leak into the surrounding tissues or blood vessel, which might cause potential safety problems and decrease the therapy efficiency. Ferromagnetic thermoseeds need precision and are relatively big, complex calculating and controlling.…”