<i>In vivo</i> measurement of bladder wall oxygen saturation using optical spectroscopy

Amelink, Arjen; Kok, Dirk J.; Sterenborg, Henricus J. C. M.; Scheepe, Jeroen R.

doi:10.1002/jbio.201100043

Cited by 10 publications

(6 citation statements)

References 38 publications

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“…To avoid artefacts caused by pressing the probe too hard to the bladder wall the probe was regularly repositioned. The experimental setup used for DPS measurements and the DPS data analysis routine was previously described in detail by Amelink et al [16,17]. Complete sessions consisted of a few hundred to more then a thousand single DPS measurements.…”

Section: Methodsmentioning

confidence: 99%

Influence of sildenafil on blood oxygen saturation of the obstructed bladder

Scheepe

Amelink²,

Wolffenbuttel

et al. 2014

BMC Urol

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BackgroundBlood oxygen saturation (BOS) is decreased in a low-compliant, overactive obstructed bladder. The objective of this study is to determine the effect of Sildenafil (SC) on bladder function and BOS) in an in vivo animal model of bladder outlet obstruction.MethodsThirty-two guinea pigs; sham operated (n = 8), sham operated + SC (n = 8), urethrally obstructed (n = 8) and urethrally obstructed + SC (n = 8) were studied during an 8 week period. BOS of the bladder wall was measured by differential path-length spectroscopy (DPS) before obstruction, at day 0, and at week 8. The bladder function was evaluated by urodynamic studies every week.ResultsBefore surgery and after sham operation all study parameters were comparable. After sham operation, bladder function and BOS did not change. In the obstructed group the urodynamic parameters were deteriorated and BOS was decreased. In the group obstruction + SC, bladder compliance remained normal and overactivity occurred only sporadic. BOS remained unchanged compared to the sham group and was significantly higher compared to the obstruction group.ConclusionsIn an obstructed bladder the loss of bladder function is accompanied by a significant decrease in BOS. Treatment of obstructed bladders with SC yields a situation of high saturation, high bladder compliance and almost no overactivity. Maintaining the microcirculation of the bladder wall might result in better bladder performance without significant loss of bladder function. Measurement of BOS and interventions focussing on tissue microcirculation may have a place in the evaluation / treatment of various bladder dysfunctions.

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Section: Methodsmentioning

confidence: 99%

Influence of sildenafil on blood oxygen saturation of the obstructed bladder

Scheepe

Amelink²,

Wolffenbuttel

et al. 2014

BMC Urol

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“…Differential path length spectroscopy (DPS) has been used to measure microvascular oxygenation in the human bladder [3]. The technique involved is invasive, but measurements are possible on both the inside and outside surfaces of the bladder wall.…”

Section: Instruments: Additional Spectroscopic Techniquesmentioning

confidence: 99%

The evolution of near infrared spectroscopy in urology

Macnab

2014

Biomedical Spectroscopy and Imaging

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In the 50 year history of NIRS, biomedical applications in urology are recent. This review summarizes technical advances and novel enquiry that have led to hemodynamic monitoring of the testis, measures of renal function, evaluation of the pelvic floor in women, and interrogation of the bladder. An overview is given of how bladder spectroscopy has evolved and the evidence that non-invasive transcutaneous optical monitoring of the anterior bladder wall now enables variations in the organ's hemodynamics and oxygen supply and demand to be identified in real time. Research indicates that bladder blood flow and oxygenation differ in health and disease, and because NIRS monitors changes in the microcirculation bladder spectroscopy adds physiologic information not available by other means, including via the current invasive method for evaluation of voiding dysfunction. Confidence that bladder NIRS yields physiologic data comes from the patterns of chromophore change and tissue oxygen saturation observed corresponding to variations in these parameters in NIRS studies of other tissues in response to known physiologic events. Importantly, when interpreted based on this prior research, novel insight is gained about probable causal pathologies underlying bladder dysfunction. Studies to date warrant further research, continued refinement of instrumentation, extension of the scope of monitoring, and exploration of diagnostic software algorithms.

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“…Hence, pathology could be performed without the need for multiple discrete and lengthy operations-in other words, much more readily and thus supposedly at reduced costs. These diagnostic technologies incorporate a range of differing tissue-imaging techniques, usually using fiber optics as a signal carrier, and they are collectively designated as "optical biopsy" [14,16].…”

Section: Introductionmentioning

confidence: 99%

Bioinspired Spring-Loaded Biopsy Harvester—Experimental Prototype Design and Feasibility Tests

Jelínek

Smit

Breedveld

2014

Journal of Medical Devices

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Current minimally invasive laparoscopic tissue–harvesting techniques for pathological purposes involve taking multiple imprecise and inaccurate biopsies, usually using a laparoscopic forceps or other assistive devices. Potential hazards, e.g., cancer spread when dealing with tumorous tissue, call for a more reliable alternative in the form of a single laparoscopic instrument capable of repeatedly taking a precise biopsy at a desired location. Therefore, the aim of this project was to design a disposable laparoscopic instrument tip, incorporating a centrally positioned glass fiber for tissue diagnostics; a cutting device for fast, accurate, and reliable biopsy of a precisely defined volume; and a container suitable for sample storage. Inspired by the sea urchin's chewing organ, Aristotle's lantern, and its capability of rapid and simultaneous tissue incision and enclosure by axial translation, we designed a crown-shaped collapsible cutter operating on a similar basis. Based on a series of in vitro experiments indicating that tissue deformation decreases with increasing penetration speed leading to a more precise biopsy, we decided on the cutter's forward propulsion via a spring. Apart from the embedded spring-loaded cutter, the biopsy harvester comprises a smart mechanism for cutter preloading, locking, and actuation, as well as a sample container. A real-sized biopsy harvester prototype was developed and tested in a universal tensile testing machine at TU Delft. In terms of mechanical functionality, the preloading, locking, and actuation mechanism as well as the cutter's rapid incising and collapsing capabilities proved to work successfully in vitro. Further division of the tip into a permanent and a disposable segment will enable taking of multiple biopsies, mutually separated in individual containers. We believe the envisioned laparoscopic optomechanical biopsy device will be a solution ameliorating time-demanding, inaccurate, and potentially unsafe laparoscopic biopsy procedures.

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In vivo measurement of bladder wall oxygen saturation using optical spectroscopy

Cited by 10 publications

References 38 publications

Influence of sildenafil on blood oxygen saturation of the obstructed bladder

Influence of sildenafil on blood oxygen saturation of the obstructed bladder

The evolution of near infrared spectroscopy in urology

Bioinspired Spring-Loaded Biopsy Harvester—Experimental Prototype Design and Feasibility Tests

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