<i>In vivo</i> maternal haploid induction in tomato

Zhong, Yu; Chen, Baojian; Wang, Dong; Zhu, Xijian; Li, Mengran; Zhang, Jinzhe; Chen, Ming; Wang, Min; Riksen, Tjitske; Liu, Jinchu; Qi, Xiaozhe; Wang, Yuwen; Cheng, D.; Liu, Zong-Kai; Li, Jinlong; Chen, Chen; Liu, Wenxin; Huang, Sanwen; Liu, Chenxu; Boutilier, Kim; Chen, Shaojiang

doi:10.1111/pbi.13755

Cited by 56 publications

(47 citation statements)

References 9 publications

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“…The FAST-Red marker facilitated efficient identification of maternal haploids in Arabidopsis and tomato (Zhong et al, 2020(Zhong et al, , 2021. However, FAST-Red was not observed in B. napus and seeds, possibly due to their thick and darker seed coat, but could be observed in germinating embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we showed that sldmp induces maternal HI in a wide range of tomato backgrounds (Zhong et al, 2021). In vivo haploids can also be induced by genome editing of DMP genes in M. truncatula (Wang et al, 2021b).…”

Section: Discussionmentioning

confidence: 99%

“…sterility (Ravi and Chan, 2010;Zhong et al, 2020Zhong et al, , 2021Wang et al, 2021b). The putative amphihaploid plants had smaller leaves and flower organs that were sterile (Figure 4).…”

Section: Identification Of Dmp Candidate Genesmentioning

confidence: 99%

“…ZmPLA1/MATL/NLD genes have only been identified in monocots (Kelliher et al, 2017; Yao et al, 2018; Liu et al, 2019), while genes related to a second maize maternal haploid inducer gene, Zea may DOMAIN OF UNKNOWN FUNCTION 679 membrane protein ( ZmDMP ), have been identified in both monocots and dicots (Zhong et al, 2020). The utility of dmp pollen for HI was recently demonstrated in the model dicot plant Arabidopsis thaliana (Zhong et al, 2020) and two dicots crops Medicago truncatula (Wang et al, 2021b) and Solanum lycopersicon (tomato) (Zhong et al, 2021), but it is not known whether this approach can be translated to other dicot crops. Here, we developed a method to identify candidate DMP genes for HI in dicot crops and demonstrated DMP ‐mediated maternal HI in two easily transformed allotetraploid crops from different plant families that are also easy to hybridize, B. napus and N. tabacum .…”

Section: Introductionmentioning

confidence: 99%

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Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Zhong

Wang

Chen

et al. 2022

JIPB

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Doubled haploid (DH) technology is used to obtain homozygous lines in a single generation, a technique that significantly accelerates the crop breeding trajectory. Traditionally, in vitro culture is used to generate DHs, but this technique is limited by species and genotype recalcitrance. In vivo haploid induction (HI) through seed is widely and efficiently used in maize and was recently extended to several other crops. Here we show that in vivo HI can be triggered by mutation of DMP maternal haploid inducer genes in allopolyploid (allotetraploid) Brassica napus and Nicotiana tabacum. We developed a pipeline for selection of DMP orthologs for clustered regularly interspaced palindromic repeats mutagenesis and demonstrated average amphihaploid induction rates of 2.4% and 1.2% in multiple B. napus and N. tabacum genotypes, respectively. These results further confirmed the HI ability of DMP gene in polyploid dicot crops. The DMP‐HI system offers a novel DH technology to facilitate breeding in these crops. The success of this approach and the conservation of DMP genes in dicots suggest the broad applicability of this technique in other dicot crops.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…sterility (Ravi and Chan, 2010;Zhong et al, 2020Zhong et al, , 2021Wang et al, 2021b). The putative amphihaploid plants had smaller leaves and flower organs that were sterile (Figure 4).…”

Section: Identification Of Dmp Candidate Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Zhong

Wang

Chen

et al. 2022

JIPB

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“…Knockout of ZmDMP results in a HIR of 0.1%-0.3% in maize, and mutations in DMP8 and DMP9 in Arabidopsis thaliana induced haploids at a rate of 2.1% ± 1.1% (Zhong et al, 2020). Mutations in DMP homologs also triggered HI in Medicago truncatula, tomato (Solanum lycopersicum), tobacco (Nicotiana tabacum), and B. napus (Wang et al, 2022;Zhong et al, 2022aZhong et al, , 2022b. However, no broad adaptation of the dmp-based HI line has been reported in B. napus, and whether there is functional redundancy among the four BnaDMP homologs has not been determined.…”

Section: Introductionmentioning

confidence: 99%

An in planta haploid induction system in Brassica napus

Xiao

et al. 2022

JIPB

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Summary Doubled haploid technology is widely used to accelerate plant breeding, but its use in the important oilseed crop Brassica napus L. is limited because B. napus haploids could only be obtained through in vitro anther or microspore cultures. Recently, maize (Zea mays) lines containing mutations in Domain of unknown function 679 membrane protein (DMP) were used as haploid inducer lines. This new haploid induction mechanism has been extended to several other plants, including the dicots Arabidopsis thaliana, tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum). Here, we knocked out four BnaDMP genes in the B. napus cultivar Westar using a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 vector with an enhanced green fluorescent protein expression cassette. Plants with DMP mutations in B. napus in the T0, T1, and T2 generations exhibited a haploid induction rate up to 2.53%. These results suggest that targeting BnaDMP could be useful for haploid induction in B. napus.

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Recent Advances in Engineering of In Vivo Haploid Induction Systems

Kelliher

2023

Methods in Molecular Biology

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In vivo maternal haploid induction in tomato

Cited by 56 publications

References 9 publications

Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

Establishment of a dmp based maternal haploid induction system for polyploid Brassica napus and Nicotiana tabacum

An in planta haploid induction system in Brassica napus

Recent Advances in Engineering of In Vivo Haploid Induction Systems

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