<i>In Vivo</i> Low‐level Light Therapy Increases Cytochrome Oxidase in Skeletal Muscle

Hayworth, Christopher R.; Rojas, Julio C.; Padilla, Eimeira; Holmes, Genevieve M.; Sheridan, Eva C.; González-Lima, Francisco

doi:10.1111/j.1751-1097.2010.00732.x

Cited by 92 publications

(58 citation statements)

References 63 publications

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“…Hayworth et al [12] reported modulations in Cox activity 24 h after the use of light therapy on the temporalis muscles in rats, but these modulations were dependent on the type of metabolism in muscle fibers that in turn suggested differences in energy synthesis (ATP). For this reason, our study assessed the effect of LEDT on ATP synthesis in muscles with either a predominance of aerobic metabolism (soleus) and mixed aerobic and glycolytic metabolism (gastrocnemius) [29].…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that taking advantage of the double absorption bands (red and near-infrared together) could optimize the effects of photobiomodulation to promote ATP synthesis and fatigue-muscle resistance. Moreover, light therapy was delivered without contact as reported previously [12,24], covering all target muscles (gastrocnemius, soleus, gluteus, and lower back muscles) and made it possible to stimulate entire muscle groups simultaneously as reported previously [31]. Finally, as the light irradiation was performed without contact, possibly reflection of the light on the animal surface (mainly on the curved areas) would have occurred in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Hayworth et al [12] used an array of LED (660 nm) to increase Cox activity after 24 h after light therapy when applied to rat muscles without contact. These authors reported differences in Cox activity depending on the type of muscle fibers.…”

Section: Introductionmentioning

confidence: 99%

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Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

et al. 2015

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Recently, low-level laser (light) therapy has been used to increase muscle performance in intense exercises. However, there is a lack of understanding of the time response of muscles to light therapy. The first purpose of this study was to determine the time response for light-emitting diode therapy (LEDT)-mediated increase in adenosine triphosphate (ATP) in the soleus and gastrocnemius muscles in mice. Second purpose was to test whether LEDT can increase the resistance of muscles to fatigue during intense exercise. Fifty male Balb/c mice were randomly allocated into two equal groups: LEDT-ATP and LEDT-fatigue. Both groups were subdivided into five equal subgroups: LEDT-sham, LEDT-5 min, LEDT-3 h, LEDT-6 h, and LEDT-24 h. Each subgroup was analyzed for muscle ATP content or fatigue at specified time after LEDT. The fatigue test was performed by mice repeatedly climbing an inclined ladder bearing a load of 150 % of body weight until exhaustion. LEDT used a cluster of LEDs with 20 red (630± 10 nm, 25 mW) and 20 infrared (850±20 nm, 50 mW) delivering 80 mW/cm 2 for 90 s (7.2 J/cm 2 ) applied to legs, gluteus, and lower back muscles. LEDT-6 h was the subgroup with the highest ATP content in soleus and gastrocnemius compared to all subgroups (P<0.001). In addition, mice in LEDT-6 h group performed more repetitions in the fatigue test (P<0.001) compared to all subgroups: LEDT-sham and LEDT-5 min (~600 %), LEDT-3 h (~200 %), and LEDT-24 h (~300 %). A high correlation between the fatigue test repetitions and the ATP content in soleus (r=0.84) and gastrocnemius (r=0.94) muscles was observed. LEDT increased ATP content in muscles and fatigue resistance in mice with a peak at 6 h. Although the time response in mice and humans is not the same, athletes might consider applying LEDT at 6 h before competition.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

et al. 2015

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“…LLLT has been found to accelerate wound healing and reduce pain, possibly by stimulating oxidative phosphorylation and reducing inflammatory responses, thus producing several beneficial effects upon inflammation and healing [10][11][12][13][14][15][16][17]. In skeletal muscle, LLLT has been effective at promoting improvements in electrical activity, preventing fatigue induced by tetanic contractions and improving healing after traumatic injury [11]. At the molecular level, LLLT directly stimulates the activity of cytochrome oxidase in muscle fibers, triggering a series of biochemical cascades that improve cellular functions [12].…”

Section: Introductionmentioning

confidence: 99%

Optical Spectroscopic Analysis of Muscle Spasticity for Low-Level Laser Therapy (LLLT)

Lee¹,

Lee²,

Youn³

2011

Journal of the Optical Society of Korea

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Current therapeutic methods for suppressing muscle spasticity are intensive functional training, surgery, or pharmacological interventions. However, these methods have not been fully supported by confirmed efficacy due to the aggravation of the muscle spasticity in some patients. In this study, a combined system was developed to treat with a low-level laser and to monitor the region of the treatment using an optical spectroscopic probe that measures oxygen saturation and deoxygenation during low-level laser therapy (LLLT). The evaluation of the wavelength dependence for LLLT was performed using a Monte Carlo simulation and the results showed that the greatest amount of heat generation was seen in the deep tissue at λ = 830 nm. In the oxy-and deoxygenation measurements during and after the treatment, oxygen-Hb concentration was significantly increased in the laser-irradiated group when compared to the control group. These findings suggest that LLLT using λ = 830 nm may be of benefit in accelerating recovery of muscle spasticity. The combined system that we have developed can monitor the physiological condition of muscle spasticity during the laser treatment in real time and may also be applied to various myotonia conditions such as muscle fatigue, back-pain treatment/monitoring, and ulcer due to paralysis.

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“…13 Following the application of photobiostimulation of tissues, a selective stimulation of mitochondrial activity is seen to occur, providing more elevated levels of cellular respiration as well as the synthesis of ATP, which results in the acceleration of mitosis. 14,15 An energy density of 5J/cm 2 was chosen for the application at each point where there was the greatest muscle contraction reaction (deep muscle fibers) to palpation of the muscles selected for this report.…”

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confidence: 99%

Photobiomodulation effect on the masseter muscle in children with cerebral palsy: a case report

et al. 2015

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Spasticity causes stiffness in the masticatory muscles of individuals with cerebral palsy (CP), affecting the amplitude of mouth opening, making oral hygiene conditions difficult and predisposing these individuals to the risk of developing oral diseases. Objective: To evaluate the effect of a low-intensity photobiomodulation diode laser on the thick part of the masseter muscle in a child with spastic type CP. Method: The caregiver reported that the child had great difficulty in performing the oral hygiene, making avoidance movements of the head when the toothbrush touched the mucosa of the upper molar region in the mouth. The mother described the child's discomfort as extreme. The first ultrasound evaluation was performed at the first appointment, and the second evaluation after 6 sessions of photobiostimulation. The low-intensity Infrared Laser Diode, Ga-As-Al, was employed (λ = 808 ± 3 nm, 120 mW; Twin Flex Evolution Laser MMOptics São Paulo, Brazil), using 5.0 J/cm 2 dose energy/location, with 20 seconds exposure/site. The area of the masseter muscle was bilaterally irradiated towards the midpoint of its length and width. Six sessions were performed, with an interval of 7 days between them. Results: At the end of the sixth session of photobiostimulation, the mother reported that the child slept better, had reduction in involuntary movements performed by the jaw, and oral hygiene was possible now with no painful expression of the child. During palpation there was less stiffness in the bilateral masseter and an increased masseter thickness and increase in the amplitude of the mouth opening of 7 mm. Conclusion: The diode laser photobiostimulation appears to be effective in reducing spasticity in the masseter muscle of children with spastic type CP.

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In Vivo Low‐level Light Therapy Increases Cytochrome Oxidase in Skeletal Muscle

Cited by 92 publications

References 63 publications

Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

Time response of increases in ATP and muscle resistance to fatigue after low-level laser (light) therapy (LLLT) in mice

Optical Spectroscopic Analysis of Muscle Spasticity for Low-Level Laser Therapy (LLLT)

Photobiomodulation effect on the masseter muscle in children with cerebral palsy: a case report

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