In vivo immunomodulatory effects of dietary purple sweet potato after immunization in chicken

Abstract

This study was intended to determine the modulatory effects of dietary supplementation of purple sweet potato (Ipomoea batats Poir., PSP) on the immune response of chickens. PSP was included in a basal starter diet by 1% (PSP(L)) or 3% (PSP(H)) and continually fed. Newcastle disease (NDV) vaccine, Brucella abortus (BA) and sheep red blood cells (SRBC) were used for chicken immunization. Antibody titers against these antigens were used to estimate humoral immunity. Concanavalin A (Con A)-induced proliferations of splenocytes, thymocytes and peripheral blood lymphocytes (PBL), ratios of CD4- and CD8-single positive and CD4-CD8-double negative (DN) cells in splenocytes, were both used to indicate cellular immunity. Relative weights of spleen, thymus and bursa and white blood cell (WBC) counts were studied. PSP(H) increased anti-NDV (P < 0.05), anti-BA (P < 0.01) and anti-SRBC titers (P < 0.05) in response to secondary immunization, whereas PSP(L) increased titers of anti-BA (P < 0.05) and anti-SRBC (P < 0.01). Proliferations of splenocytes and thymocytes were augmented with PSP(L) (P < 0.05). PSP(H)-treated chickens had lower (P < 0.05) ratios of CD4-single positive lymphocytes. Proliferation of PBL, weights of lymphoid organs and WBC counts were not affected. These results suggest that dietary PSP supplementation could enhance the immune response after immunization in chickens.

Immunomodulatory and Antioxidant Effects of Purple Sweet Potato Extract in LP-BM5 Murine Leukemia Virus-Induced Murine Acquired Immune Deficiency Syndrome

“…the control group in immunized chickens. 26 The prior and present data establish the immunomodulatory effect of purple sweet potato due to inhibition of apoptosis during LP-BM5 MuLV infection.…”

A review of therapeutic potentials of sweet potato: Pharmacological activities and influence of the cultivar

“…Ethyl acetate fractions of bioactives extracted from two different cultivars of sweet potato exhibited immunomodulatory activities in a cultivar dependent manner in mice splenocytes [56]. Hanieh et al [57] reported that dietary supplementation of purple sweet potato improved immune response after immunization in chickens. Similarly, the consumption of purple sweet potato leaves was able to modulate Tlymphocyte functions, lytic activity of natural killer cell and antibody production in a study involving 16 healthy human adults [58].…”

Immunomodulatory effects of Alliums and Ipomoea batata extracts on lymphocytes and macrophages functions in White Leghorn chickens: In vitro study

“…In the same study it was reported that dietary supplement with polyphenol resulted in a higher proliferation of lymphocytes, IL‐2 secretion and lytic activity of NK cells. It is likely that the high contents of flavonoids in onion and PSP that contributed to the inhibitory effects in vitro , also contributed to the stimulatory effects in vivo that we previously reported (Hanieh et al . 2010a,b).…”

“…In this context, we previously reported that dietary garlic, onion (Hanieh et al . 2010b) and PSP (Hanieh et al . 2010a), improved the humoral immune response against three different antigens.…”