In vivoImaging β-cell Function Reveals Two Waves of β-cell Maturation

Abstract

17The insulin-secreting cells generated from stem cells in vitro are less glucose responsive than 18 primary β-cells. To search for the missing ingredients that are needed for β-cell maturation, we 19 have longitudinally monitored function of every β-cell in Tg (ins:Rcamp1.07) zebrafish embryos 20 with a newly-invented two-photon light-sheet microscope. We have shown that β-cell maturation 21 begins from the islet mantle and propagates to the islet core during the hatching period, coordinated 22 by the islet vascularization. Lower concentration of glucose is optimal to initiate β-cell maturation,