<i>In Vivo</i>Imaging of Reactive Oxygen Species in Mouse Brain by using [<sup>3</sup>H]Hydromethidine as a Potential Radical Trapping Radiotracer

Abe, Kohji; Takai, Nobuhiko; Fukumoto, Kazumi; Imamoto, Natsumi; Tonomura, Misato; Ito, Miwa; Kanegawa, Naoki; Sakai, K.; Morimoto, Kenji; Todoroki, Kenichiro; Ito, Osamu

doi:10.1038/jcbfm.2014.160

Cited by 25 publications

(25 citation statements)

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“…20 A number of radiolabeled analogues of dihydro-methidium (also known as hydromethidine) have been synthesized and evaluated in vitro and in vivo, including [ 3 H]hydromethidine and [ 11 C]hydromethidine. 21,22 [ 3 H]Hydromethidine is oxidized by superoxide and hydroxyl radicals and has been evaluated in animal models of stroke and cisplatin-induced nephrotoxicity. 23,24 A recent paper on the radiosynthesis and preliminary in vivo evaluation of [ 11 C]methidium has also been reported.…”

Section: Discussionmentioning

confidence: 99%

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

Hou

Hsieh

et al. 2017

ACS Chem. Neurosci.

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Reactive oxygen species (ROS) are believed to play a major role in the proinflammatory, M1-polarized form of neuroinflammation. However, it has been difficult to assess the role of ROS and their role in neuroinflammation in animal models of disease because of the absence of probes capable of measuring their presence with the functional imaging technique positron emission tomography (PET). This study describes the synthesis and in vivo evaluation of [18F]ROStrace, a radiotracer for imaging superoxide in vivo with PET, in an LPS model of neuroinflammation. [18F]ROStrace was found to rapidly cross the blood–brain barrier (BBB) and was trapped in the brain of LPS-treated animals but not the control group. [18F]ox-ROStrace, the oxidized form of [18F]ROStrace, did not cross the BBB. These data suggest that [18F]ROStrace is a suitable radiotracer for imaging superoxide levels in the central nervous system with PET.

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Section: Discussionmentioning

confidence: 99%

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

Hou

Hsieh

et al. 2017

ACS Chem. Neurosci.

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“…Molecular imaging of ROS/RNS is an emerging area of research in redox and free radical biology [6–8]. Bioluminescence or fluorescence modalities are typically used.…”

Section: Introductionmentioning

confidence: 99%

On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo – Identification of reaction intermediates and oxidant-specific minor products

Zielonka

Podsiadly

Zielonka

et al. 2016

Free Radical Biology and Medicine

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Peroxy-caged luciferin (PCL-1) probe was first used to image hydrogen peroxide in living systems (Van de Bittner GC et al. Proc Natl Acad Sci U S A. 2010; 107:21316–21). Recently this probe was shown to react with peroxynitrite more potently than with hydrogen peroxide (Sieracki et al. Free Radic Biol Med. 2013; 61:40–50) and was suggested to be a more suitable probe for detecting peroxynitrite under in vivo conditions. In this work, we investigated in detail the products formed from the reaction between PCL-1 and hydrogen peroxide, hypochlorite, and peroxynitrite. HPLC analysis showed that hydrogen peroxide reacts slowly with PCL-1, forming luciferin as the only product. Hypochlorite reaction with PCL-1 yielded significantly less luciferin, as hypochlorite oxidized luciferin to form a chlorinated luciferin. Reaction between PCL-1 and peroxynitrite consists of a major and minor pathway. The major pathway results in luciferin and the minor pathway produces a radical-mediated nitrated luciferin. Radical intermediate was characterized by spin trapping. We conclude that monitoring of chlorinated and nitrated products in addition to bioluminescence in vivo will help identify the nature of oxidant responsible for bioluminescence derived from PCL-1.

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“…This enabled the imaging of O 2 •− levels under stimulation by different drugs in vivo in zebra fish and mice. Moreover, Inoue and co‐workers reported the in vivo imaging in a mouse brain using a hydromethidine probe.…”

Section: Fluorescent Imaging Agents For Diagnosis Of Ros‐relevant Dismentioning

confidence: 99%

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

et al. 2019

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Reactive oxygen species (ROS) consist of a diverse range of oxidative small molecular ions and free radicals that are produced throughout the body during certain biological processes. Due to their high reactivity, these molecules result in the damage of tissues and cells. Therefore, ROS have been implicated in an array of diseases including cancer, inflammation, and neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Owing to the simplicity, sensitivity, and selectivity of fluorescence‐based strategies, many small‐molecular sensors or imaging agents have been developed to sense and visualize ROS both in vitro and in vivo. Likewise, activatable drug delivery and prodrug systems that can be triggered by ROS for disease theranostics (diagnostic and therapeutic combined) have been developed. Herein, recently developed fluorescence‐based sensing and imaging agents for the detection of ROS both intracellularly and in vivo are summarized. In addition, drug delivery, which require activation by ROS to achieve disease theranostics is also discussed.

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In VivoImaging of Reactive Oxygen Species in Mouse Brain by using [³H]Hydromethidine as a Potential Radical Trapping Radiotracer

Cited by 25 publications

References 35 publications

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo – Identification of reaction intermediates and oxidant-specific minor products

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

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In VivoImaging of Reactive Oxygen Species in Mouse Brain by using [3H]Hydromethidine as a Potential Radical Trapping Radiotracer

Cited by 25 publications

References 35 publications

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo – Identification of reaction intermediates and oxidant-specific minor products

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

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In VivoImaging of Reactive Oxygen Species in Mouse Brain by using [³H]Hydromethidine as a Potential Radical Trapping Radiotracer