<i>In Vivo</i> Imaging of Nitric Oxide by Magnetic Resonance Imaging Techniques

Sharma, Rakesh; Seo, Jeong-Won; Kwon, Soonjo

doi:10.1155/2014/523646

Cited by 14 publications

(7 citation statements)

References 109 publications

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“…For example, the colorimetric Griess assay is useful for analyzing NO in cell lysates; however, this is performed indirectly via detection of nitrite and nitrate and requires acidic conditions that are not biocompatible . In contrast, techniques such as electron paramagnetic resonance (EPR) spectroscopy and magnetic resonance imaging (MRI) have been employed for NO detection in vivo at relevant imaging depths; however, these approaches are limited by low resolution and sensitivity, respectively . Amperometry displays high sensitivity (pM) but requires invasive procedures and can only detect NO in direct contact with the probe. − Optical methods such as luminescence and fluorescence imaging are noninvasive and enable high resolution with high contrast at shallow imaging depths; as a result, a diverse palette of reaction-based fluorescent probes has been developed, primarily for cellular studies.…”

Section: Introductionmentioning

confidence: 99%

A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

et al. 2018

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Photoacoustic (PA) imaging is an emerging imaging modality that utilizes optical excitation and acoustic detection to enable high resolution at centimeter depths. The development of activatable PA probes can expand the utility of this technology to allow for detection of specific stimuli within live-animal models. Herein, we report the design, development, and evaluation of a series of Acoustogenic Probe(s) for Nitric Oxide (APNO) for the ratiometric, analyte-specific detection of nitric oxide (NO) in vivo. The best probe in the series, APNO-5, rapidly responds to NO to form an N-nitroso product with a concomitant 91 nm hypsochromic shift. This property enables ratiometric PA imaging upon selective irradiation of APNO-5 and the corresponding product, tAPNO-5. Moreover, APNO-5 displays the requisite photophysical characteristics for in vivo PA imaging (e.g., high absorptivity, low quantum yield) as well as high biocompatibility, stability, and selectivity for NO over a variety of biologically relevant analytes. APNO-5 was successfully applied to the detection of endogenous NO in a murine lipopolysaccharide-induced inflammation model. Our studies show a 1.9-fold increase in PA signal at 680 nm and a 1.3-fold ratiometric turn-on relative to a saline control.

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Section: Introductionmentioning

confidence: 99%

A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

et al. 2018

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“…This low concentration might be used as a threshold value in images of NO concentration inside and outside the endothelial cells (as well as other brain cells). For instance, certain fluorescein biosensors can be used as NO probes for real-time visualization of the spatiotemporal distribution of NO in fluoroscopy (Sharma et al, 2014), while manganese-based NO-selective contrast agents can help with magnetic resonance images (MRI) of NO (Barandov et al, 2020). These biosensors are chemical agents that could be delivered to the brain either via the intracerebrospinal fluid or by intravascular injection.…”

Section: Discussionmentioning

confidence: 99%

Modeling NO Biotransport in Brain Using a Space-Fractional Reaction-Diffusion Equation

Tamis

Drapaca

2021

Front. Physiol.

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Nitric oxide (NO) is a small gaseous molecule that is involved in some critical biochemical processes in the body such as the regulation of cerebral blood flow and pressure. Infection and inflammatory processes such as those caused by COVID-19 produce a disequilibrium in the NO bioavailability and/or a delay in the interactions of NO with other molecules contributing to the onset and evolution of cardiocerebrovascular diseases. A link between the SARS-CoV-2 virus and NO is introduced. Recent experimental observations of intracellular transport of metabolites in the brain and the NO trapping inside endothelial microparticles (EMPs) suggest the possibility of anomalous diffusion of NO, which may be enhanced by disease processes. A novel space-fractional reaction-diffusion equation to model NO biotransport in the brain is further proposed. The model incorporates the production of NO by synthesis in neurons and by mechanotransduction in the endothelial cells, and the loss of NO due to its reaction with superoxide and interaction with hemoglobin. The anomalous diffusion is modeled using a generalized Fick’s law that involves spatial fractional order derivatives. The predictive ability of the proposed model is investigated through numerical simulations. The implications of the methodology for COVID-19 outlined in the section “Discussion” are purely exploratory.

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“…Emerging imaging techniques could help validate the proposed model in animal models and thus make the model relevant to clinical applications. For instance, a multimodal in vivo magnetic resonance (MR)/electron paramagnetic resonance (EPR) spectroscopy/fluorometry could be used to visualize NO production and spatio-temporal distribution (Sharma et al, 2014). Also, intravascular optical coherence tomography could be used for the in vivo real-time estimation of the vascular stiffness (Potlov et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

A Nitric Oxide–Modulated Variable-Order Fractional Maxwell Viscoelastic Model of Cerebral Vascular Walls

Drapaca

2021

Front. Mech. Eng.

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It is well known that the mechanical behavior of arterial walls plays an important role in the pathogenesis of vascular diseases. Most studies existing in the literature focus on the mechanical interactions between the blood flow and wall’s deformations. However, in the brain, the smaller vessels experience not only oscillatory forces due to the pulsatile blood flow but also structural and morphological changes controlled by the surrounding brain cells. In this study, the mechanical deformation of the cerebral arterial wall caused by the pulsatile blood flow and the dynamics of the neuronal nitric oxide (NO) is investigated. NO is a small diffusive gaseous molecule produced by the endothelial cells and neurons, which is involved in the regulation of cerebral blood flow and pressure. The cerebral vessel is assumed to be a hollow axial symmetric cylinder whose wall thickness is much smaller than the cylinder’s radius and longitudinal length is much less than the propagating wavelength. The wall is an isotropic, homogeneous linear viscoelastic material described by an NO-modulated variable-order fractional Maxwell model. A fractional telegraph equation is obtained for the axial component of the displacement. Patterns of wall’s deformation are investigated through numerical simulations. The results suggest that a significantly decreased inactivation of the neuronal NO may cause a reduction in the shear stress at the blood-vessel interface, which could lead to a decrease in the production of shear-induced endothelial NO and neurovascular disease.

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In Vivo Imaging of Nitric Oxide by Magnetic Resonance Imaging Techniques

Cited by 14 publications

References 109 publications

A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

A Ratiometric Acoustogenic Probe for in Vivo Imaging of Endogenous Nitric Oxide

Modeling NO Biotransport in Brain Using a Space-Fractional Reaction-Diffusion Equation

A Nitric Oxide–Modulated Variable-Order Fractional Maxwell Viscoelastic Model of Cerebral Vascular Walls

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