<i>In vivo</i> Fertilizing Capacity of Deep Frozen Boar Semen Packaged in Plastic Bags and Maxi‐Straws

Bwanga, C. O.; Hofmo, P. O.; Grevle, IS; Einarsson, S.; Rodriguez‐Martinez, Heriberto

doi:10.1111/j.1439-0442.1991.tb01014.x

Cited by 12 publications

(9 citation statements)

References 12 publications

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“…With cryopreserved sperm use in gilts, fertilization and embryo recovery rates have been reported at 60–70%, which are similar to those for data in sows and for fertility when using fresh semen (Bwanga et al. ; McNamara and Knox ; Ringwelski et al. ).…”

Section: Artificial Insemination With Cryopreserved Boar Spermsupporting

confidence: 76%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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Contents One of the limits to practical use of frozen boar sperm involves the lowered fertility when used for artificial insemination. Years of studies have shown that 5–6 billion sperm (approximately 3 billion viable) used in single or multiple inseminations results in pregnancy rates most often between 60 and 70% and with litter sizes between nine and 10 pigs. Yet today, it is not uncommon for studies to report pregnancy rates from 70 to 85% and litter sizes with 11–12 pigs. While global statements about the incidence and reasons for higher fertility are not conclusive, incremental fertility improvements appear independently associated with use of a minimum number of viable sperm (1–2 billion), insemination timing that increases the probability that sperm will be present close to ovulation for groups of females, selection for boar sperm survival following cryopreservation, and modification of the freeze and thaw conditions using additives to protect sperm from oxidative damage. Studies show that techniques such as intrauterine and deep uterine insemination can provide an opportunity to reduce sperm numbers and that control of time of ovulation in groups of females can reduce the need for multiple inseminations and improve the chance for AI close to ovulation. However, optimal and consistent fertility with cryopreserved boar sperm may require a multifaceted approach that includes boar selection and screening, strategic use of additives during the freezing and thawing process, post‐thaw evaluation of sperm and adjustments in sperm numbers for AI, assessment of female fertility and ovulation induction for single insemination. These sequenced procedures should be developed and incorporated into a quality control system for improved fertility when using minimal numbers of cryopreserved boar sperm.

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Section: Artificial Insemination With Cryopreserved Boar Spermsupporting

confidence: 76%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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“…In contrast, in the present study motility rates of approximately 47% were obtained, which were similar to other reported values in 0.25 and 0.5 ml straws (47.7–52.6%) (Bwanga et al. 1990, 1991a, b). For 5 ml straws, the same authors found values ranging from 29.9 to 40.1%, whereas values of approximately 47% were obtained in the present study.…”

Section: Discussionsupporting

confidence: 90%

“…On the other hand, whereas the NAR values obtained by most authors were 59.4–84.5% for maxi‐, medium‐ and ministraws (Bwanga et al. 1990, 1991a,b; Torreta et al. 1996), in the present study lower values (46–48%) were obtained in the two straw sizes.…”

Section: Discussioncontrasting

confidence: 85%

“…When using the same volume, 5 ml, significantly higher motility and NAR were present in flat plastic bags than in maxi‐straws (Bwanga et al. 1991a,b). However in this study, no significant differences were found between 0.5 and 5 ml straws, regarding both motility and NAR.…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies related to the influence of the volume of the straw on boar frozen semen quality have evaluated in vitro motility rates and normal apical ridge (NAR) percentages as well as in vivo fertility rates (Hammit and Martin 1989; Bwanga et al. 1990, 1991b). However, there are other parameters related to fertility than can be measured in vitro to evaluate the semen quality, such as monospermy, polyspermy and no fertilization rates.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Fertilizing Capacity of Deep Frozen Boar Semen Packaged in 0.5 and 5 ml Straws

Córdova

Pérez

Lleó

et al. 2001

Reprod Domestic Animals

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The aim of this study was to evaluate the straw size effect used for freezing on the in vitro fertilizing capacity. Twenty-one ejaculates from seven fertile boars were frozen under controlled conditions in 0.5 and 5 ml straws. Thawed semen was compared to fresh semen. For fresh and thawed semen in 0.5 and 5 ml straws, the results were: 92.18, 77.38 and 79.04% sperm penetration; 80.68, 66.89 and 69.33% monospermy; 11.51, 10.49 and 9.74% polyspermy; 86.19, 47.14 and 47.02% motility and 75.52, 48.19 and 46.81% normal apical ridge (NAR), respectively. Analysis of variance and test of multiple comparisons showed that under the conditions employed, penetration, monospermy, motility and NAR were significantly reduced by freezing-thawing, but polyspermy was much less affected. The results obtained suggest that frozen boar semen is adequate for in vitro fertilization. In addition freezing in 5 ml straws did not have any detrimental effect on either penetration, monospermy, polyspermy, motility and NAR, in comparison with freezing in 0.5 ml straws.

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Artificial insemination with frozen‐thawed boar sperm

Yeste

Rodríguez-Gil

Bonet

2017

Molecular Reproduction Devel

104

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Artificial insemination with frozen-thawed semen in pigs is not a routine technique; its use is restricted to specific cases, such as preservation of valuable genetic material (germplasm banks), safety strategies in case of natural disasters, long-distance transport of sperm, and in combination with sex-sorting. Cryoinjuries resulting from freeze-thawing protocols are a major concern with regard to the fertilization capacity of the treated sperm, which is lower than that of liquid-stored semen. Here, we provide an overview of artificial insemination using cryopreserved sperm, and summarize the factors that influence cryopreservation success before, during, and after freeze-thaw (i.e., sperm selection before starting the cryopreservation process, holding time, use of cryoprotectants, and rates of freezing and thawing) and that are driving the identification of biomarkers to predict sensitivity to cryodamage. Three different artificial insemination techniques (conventional or intracervical; intrauterine; and deep intrauterine) are also discussed with regards to their relevance when using frozenthawed semen. Finally, we review the use of additives to freezing and thawing media, given reports that they may maintain and improve the quality and fertilizing capacity of frozen-thawed sperm. In sum, artificial insemination with frozen-thawed boar sperm can provide reasonable fertility outcomes, if freezable ejaculates, specific additives, and appropriate insemination techniques are used.State-of-the-art of artificial insemination with frozenthawed sperm still suffers from the negative impact that cryopreservation has on the sperm itself.

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In vivo Fertilizing Capacity of Deep Frozen Boar Semen Packaged in Plastic Bags and Maxi‐Straws

Cited by 12 publications

References 12 publications

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

In vitro Fertilizing Capacity of Deep Frozen Boar Semen Packaged in 0.5 and 5 ml Straws

Artificial insemination with frozen‐thawed boar sperm

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