In vivo evaluation of serotonergic agents and α‐adrenergic blockers on premature ejaculation by inhibiting the seminal vesicle pressure response to electrical nerve stimulation

Abstract

Objective To evaluate the eCect of drugs on premature (8.9)% by fluoxetine at 0.1 mg/kg, 67.9 (8.7)% by prazosin at 0.1 mg/kg, 60.9 (11.0)% by serotonin at ejaculation using a rat animal model in which the seminal vesicle was electrically stimulated via its lesser 3 mg/kg, 54.9 (4.6)% by clomipramine at 3 mg/kg, 30.0 (11.0)% by imipramine at 0.1 mg/kg, and 20.9 splanchnic nerve and changes in the pressure response monitored.(4.3)% by indatraline at 0.1 mg/kg. From the concentration-response curve, the potency of prazosin was Materials and methods Male Wistar rats (aged 12-14 weeks) were injected intravenously with prazosin and lower than that of fluoxetine, but was higher than that of serotonin or clomipramine. serotonergic agents (serotonin, clomipramine, fluoxetine, imipramine and indatraline) at various concen-Conclusions Like serotonin, fluoxetine and clomipramine can reduce the pressure response of the seminal trations 10 min before electrical nerve stimulation (ENS) of the lesser splanchnic nerve; the initial increase vesicle to ENS. Among these inhibitory agents, including prazosin, fluoxetine was the most eCective and in seminal vesicle pressure in response to ENS was then compared. may be valuable for the clinical treatment of ejaculatory dysfunction in man. Results The pressure response to ENS was reduced in the presence of prazosin or serotonergic agents. The Keywords Lesser splanchnic nerve, premature ejaculation, seminal vesicle, serotonin, Wistar rat mean (SEM) maximum inhibition values were 84.1 tonergic system inhibits the contraction of the seminal

Neuroanatomy and neurophysiology related to sexual dysfunction in male neurogenic patients with lesions to the spinal cord or peripheral nerves

“…1,15,16 Some solutions are also available for premature ejaculation, such as SSRI. 34,42,48,53,54 Potential new treatments for premature ejaculation to be explored are sildenafil and tramadol (SSRI-like effect). 34,42,48,55 However, a valid pharmaceutical option for inducing antegrade ejaculation or treating anejaculation does not exist.…”

Seminal Plug Expulsion Induced by Electrical Stimulation of the Intermesenteric Nerve in Anesthetized Rats

“…The ability of IMN electrical stimulation to induce ejaculation in anesthetized rats has not been previously reported, although the role of the sympathetic pathway, including IMNs and HNs, in the control of bladder neck closure, contraction of seminal vesicles, and seminal fluid secretion has been demonstrated in dogs and rats [15][16][17]. The IMN connects the celiac superior mesenteric ganglion to the inferior mesenteric ganglion.…”

In vivo rat model to measure hypogastric nerve stimulation-induced seminal vesicle and vasal pressure responses simultaneously

“…Thus, the development of an in vivo experimental model for assessing the efficacy of these new drugs is a pressing matter. Hsieh et al 6 proposed a rat model involving electrical stimulation of the lesser splanchnic nerve to induce changes in the intraluminal pressure of the seminal vesicle. They measured the inhibition of electrical stimulationinduced seminal vesicle pressure increases by several agents (prazosin, 5-HT, clomipramine, fluoxetine, imipramine, and indatraline).…”

“…Previous efforts have been made to develop an in vivo model to measure the contractile response in the seminal vesicle or vas deferens to electrical nerve stimulation in order to evaluate therapeutic efficacy of drugs for premature ejaculation. 6,7 Aside from the differences between these models in terms of target organs and stimulated nerve, there were qualitative differences in the findings of these two reports.…”