<i>In Vivo</i> Estrogenic Activity of Nonylphenol in Rainbow Trout

Lech, John J.; Lewis, Steven K.; Ren, Lefen

doi:10.1093/toxsci/30.2.229

Cited by 74 publications

(62 citation statements)

References 8 publications

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“…o,p'-DDT and nonylphenol are environmental contaminants with reported itrol estrogenic activity (14)(15)(16)(17)(18)(19) of the test systems and to proOver the same dose range where effects e data against which to comwere seen with nonylphenol, o,p'-DDT Its of the other eight chemi-gave a greater response in the receptor bind-*oduced the effects expected, ing assay (Fig. 2).…”

Section: Resultsmentioning

confidence: 90%

Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays.

Shelby

Newbold

Tully

et al. 1996

Environ Health Perspect

386

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Section: Resultsmentioning

confidence: 90%

Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays.

Shelby

Newbold

Tully

et al. 1996

Environ Health Perspect

386

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“…NP can compete with estradiol for binding to the ER and demonstrate estrogen-like activity both in vivo and in vitro with potencies of approximately 10 -4 to 10 -7 relative to estradiol [44][45][46][47][48]. There are some reports showing PR expression by NP in MGs.…”

Section: Figmentioning

confidence: 99%

Gestational Exposure to Nonylphenol Causes Precocious Mammary Gland Development in Female Rat Offspring

Moon

Han

Shin

et al. 2007

Journal of Reproduction and Development

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Abstract. This study examined whether or not exposure to 4-nonylphenol (NP) during late gestation affects reproductive and mammary development in the offspring of female rats. Time pregnant Long Evans rats were gavaged with NP (10 or 100 mg/kg), atrazine (ATR, 100 mg/kg), or corn oil on gestation days 15-19. The uterus weights of the NP (100 mg/kg/d)-exposed pups were higher than those of the controls but the weights of the other organs were unchanged. Delayed mammary gland (MG) development was detected in the ATR pups on PND 4 and persisted through to PND 66. The high dose NP pups had advanced lobular development of their MG on PND 22, while the glands from the low dose NP pups were no different morphologically from the controls. Immunohistochemical comparisons of the mammary sections from PND 41 demonstrated low levels of estrogen receptor (ER) staining in the control gland stroma and epithelium but higher levels in the tissue of the pups exposed to NP and ATR. ATR also elevated ER in the stroma surrounding the epithelial layer of the terminal end buds. The level of progesterone receptor (PR) staining was markedly lower in the epithelium of the 100 mg/kg NP glands vs. the control glands. However, PR was present at high levels in the epithelium of the 10 mg/kg NP glands and was even more prominent in the ATRexposed ductal epithelium and fat cell nuclei. The level of prolactin staining was only elevated in glands containing lobule areas (NP-exposed) compared with the control levels. These results suggest that NP and ATR have opposite effects on the development of MG after gestational exposure. Exposure to them during the critical period of epithelial outgrowth altered the receptor levels of mammary progesterone and prolactin and might contribute to the differences in the mammary morphology at PND 41. Key words: 4-Nonylphenol (NP), Atrazine, Endocrine disruptor, Hormone receptor, In utero exposure, Mammary gland, Rat (J. Reprod. Dev. 53: [333][334][335][336][337][338][339][340][341][342][343][344] 2007) he branched-chain isomer 4-nonylphenol (NP) is metabolized microbiologically from nonionic surfactants, 4-nonylphenol ethoxylates [1,2], and exhibits estrogenic eff e c t s b y s t i m u la t i n g expression of the estrogen-responsive vitellogenin gene in fish [3][4][5] and growth of human breast c a n c e r M C F -7 c e l l s [ 6 ] . N P a l s o e x e r t e d uterotrophic activity in immature and mature ovariectomised female rats and induced early onset of vaginal opening in a 20-day pubertal assay [7-

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“…& Fax: +81-944-53-8747; E-mail: tominaga@ariake-nct.ac.jp mans. 2,3) Therefore, the estrogenic effects and their intensity must be evaluated in order to confirm the safety of both preexisting and novel chemicals.…”

Section: Introductionmentioning

confidence: 99%

Expression Analysis of Estrogen-responsive Genes Vitellogenin 1 and 2 in Liver of Male Medaka (Oryzias latipes) Exposed to Selective Ligands of Estrogen Receptor Subtypes

Yamaguchi

Ishibashi

Kohra

et al. 2009

JOURNAL OF HEALTH SCIENCE

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Vitellogenin (VTG) is a useful biomarker for detecting the estrogenic activity of chemicals in aquatic environments. However, little information is available on the regulatory mechanisms of the expression of each VTG subtype, particularly the relationship between expression patterns of VTG1/2 and estrogen receptor (ER) subtypes, such as ERα and ERβ. In this paper, we measured VTG1 and VTG2 mRNA induction in male medaka liver, which was treated with ERα-selective ligand, (17α, 20E)-3-hydroxy-17,20-[(1-methoxyethylidene)bis(oxy)]-19-norpregna-1,3,5(10),20-tetraene-21-carboxylic acid, methyl ester or ERβ-selective ligand, 2-(4-hydroxyphenyl)-5-hydroxy-1,3-benzoxazole and investigated the characteristics of ER subtype function in VTG1 and VTG2 inductions. Hepatic VTG1 mRNA was induced by ERα-selective ligands at even low concentration and maximum increases were the same as for E2. VTG2 mRNA was also increased, but its levels were very low. On the other hand, ERβ-selective ligands significantly increased VTG2 mRNA in the presence of ERα agonists. These results indicate that the expression of each VTG subtype is regulated by unique ER subtypes. VTG1 expression is only regulated by the action of ERα. In contrast, VTG2 expression is regulated by both ERα and ERβ, with ERα being essential for VTG2 gene expression and ERβ being essential for enhancement.

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In Vivo Estrogenic Activity of Nonylphenol in Rainbow Trout

Cited by 74 publications

References 8 publications

Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays.

Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays.

Gestational Exposure to Nonylphenol Causes Precocious Mammary Gland Development in Female Rat Offspring

Expression Analysis of Estrogen-responsive Genes Vitellogenin 1 and 2 in Liver of Male Medaka (Oryzias latipes) Exposed to Selective Ligands of Estrogen Receptor Subtypes

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