In vivo Electrochemical Monitoring of Signaling Transduction of Plant Defense Against Stress in Leaves of Aloe vera L.

Abstract

In situ recording of the voltammetric response of Aloe vera L. leaves using graphite and platinum microelectrodes inserted in the subcuticular region is described. The plant response against the mechanical stress results in changes of the voltammetric signals associated to signal transduction compounds salicylic and jasmonic acids and hydrogen peroxide, which were separately monitored. A kinetic model is proposed to describe the time variation of the respective voltammetric signatures, denoting the possibility of in vivo electrochemical monitoring of biochemical processes in plants. The obtained results suggest that two defense pathways with different participation of ROS are operative.

In situ electrochemical monitoring of signaling transduction in plants. Implications on phylogenetic aspects of defense response

“…This behavior differs significantly from that previously reported for Aloe vera , where the R ox signal increases progressively until reaching a maximum after ca. 25 min further decreasing slowly [42]. The peak currents measured under our experimental conditions differ slightly but significantly in replicate experiments performed in different leaves of the same plant.…”

“…For our purposes, it is interesting to investigate the possible coupling between SA, JA and H 2 O 2 production under stress. Our data depict a more complex scenario than that described in our previous study on Aloe vera [42]. The relevant point to emphasize is that the behavior of the different plants is reflected in differences between the ‘fast’ generation pathway (Q‐path, defined by k Q ) and the ‘slow’ generation pathway (P‐path, defined by k P ).…”

“…In this context, previous works revealed the suitability of electrochemical techniques to acquire information on the role of gynodioecy in adaptation to stress [37], response to herbivory [38] and chemotaxonomic and phylogenetic information [39][40][41]. More recently, an in situ study on the kinetics of the response of Aloe vera L. to mechanical stress, suggested that the signaling pathways of JA and SA operate independently with different kinetics [42]. These results also suggested the possibility of extracting phylogenetic information in terms of the coupling/decoupling relationships between the different defense pathways.…”

In Vivo Detection of Glutamate in Tomatoes by an Enzyme-Based Electrochemical Biosensor

“…Electrochemical sensors are one of the most potential approaches for in vivo and on-site monitoring of biomolecules, because of their simplicity, sensitivity, portability, and easy-to-miniaturize and -integrate nature. − Several in vivo electrochemical sensors for glutamate have been developed. For example, for in vivo glutamate monitoring in spinal cord, Nguyen et al have fabricated a flexible glutamate biosensor using a simple direct ink writing technique .…”

Voltammetric Electrochemical Sensor for Phylogenetic Study in Acer Linn.

“…Electrochemical fingerprinting is a technique for collecting electrochemically active substances in plant tissues. Since the electrochemical signal is proportional to the type and content of the substance, it can reflect the difference of electrochemically active substance profile in plants [11][12][13][14][15][16][17][18][19][20][21][22][23]. For example, Doménech-Carbó et al [14] demonstrated the voltammetric fingerprints of Asparagus seeds can reflect the profiles of polyphenolic compounds (daidzein, ellagic acid, gallic acid, genistein, morin, quercetin, and rutin).…”