<i>In Vivo</i> Delivery of Nanoparticles into Plant Leaves

Wu, Honghong; Santana, Israel; Dansie, Joshua; Giraldo, Juan Pablo

doi:10.1002/cpch.29

Cited by 31 publications

(24 citation statements)

References 40 publications

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“…To investigate the uptake, transport, and distribution of nanoparticles in plants, and the impact of nanomaterials on plant function, the first key step is to deliver the nanoparticles into plants in vivo [45]. Uptake and accumulation of carbon nanotubes along with fullerenes and fullerol in edible and crop plants were previously reported [46].…”

Section: N-cds' Insertion Into Leaf Cellsmentioning

confidence: 99%

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Bajpai

D'Souza²,

Suhail

2019

Int Nano Lett

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The milk protein casein (Cas) has been employed as carbon resource material to synthesize nitrogen-doped carbon dots (N-CDs) via microwave exposure. The dots, when exposed to UV light, produced blue fluorescence. The N-CDs were characterized by ultra violet (UV) spectroscopy, Fourier transformation infrared spectroscopy, X-ray diffraction (XRD), dynamic light scattering analysis, fluorescent microscopy (FM), and transmission electron microscopy (TEM). The XRD analysis revealed a broad peak at 2θ = 20°, thus indicating the turbostratic carbon phase. TEM analysis and particle size distribution curve revealed that nearly, 85% of the particles had diameter below 10 nm and the particles had spherical geometry. The HRTEM analysis revealed that carbon dots exhibited lattice fringes with a d-spacing of 0.21 nm, corresponding to the (100) plane lattice of graphite. The fluorescence spectral studies indicated a red shift in the emission peak from 420 to 450 nm as the excitation wavelength increased from 300 to 340 nm. The zeta potential of particles was found to be -11.3 mV. Finally, impregnation of N-CDs was studied in Spinacia oleracea leaf. It was observed that as the concentration of N-CDs' solution increased, percent insertion (PI) also increased, but the time required for maximal insertion decreased with increasing concentrations of N-CDs in the feed solutions. In the carbon dots' solution with a concentration of 200 ppm, maximum percent insertion (MPI) was obtained after 80 min. However, with the increasing concentration of N-CDs in the feed solutions, time of getting MPI reduced, i.e., in 600 ppm, it was 30 min, and in 800 ppm, it was 10 min.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: N-cds' Insertion Into Leaf Cellsmentioning

confidence: 99%

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Bajpai

D'Souza²,

Suhail

2019

Int Nano Lett

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“…Plant growth. Plants were grown in Adaptis 1000 growth chambers (Conviron) using standard conditions 55 . Growth chamber conditions were 200 μmol m −2 s −1 PAR, 24 ± 1 and 21 ± 1°C day/night, 60% humidity, and 14/10 h day/night regime.…”

Section: Methodsmentioning

confidence: 99%

“…The Chl-QD solution was diluted to 200 nM (0.17 mg mL −1 ) and loaded with 60 µM methyl viologen or ascorbic acid. Nanoparticle solution was infused through the abaxial side of the leaf using a 1 mL needles syringe 55,65 . Approximately 100 μL solution was perfused into each plant leaf by gently pressing the tip of the syringe against the bottom of the leaf lamina and depressing the plunger.…”

Section: Methodsmentioning

confidence: 99%

Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif

et al. 2020

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Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function. We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif. Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles. QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts. Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone. Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.

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“…It is suggested that NPs within a size range limit according to the cell wall can traverse through it. For example, quantum dots (QDs) fall under the size range of plant cell wall pores (sub-10 nm) that can be easily exploited to make their entry through the plants (Wu et al, 2017a). Li et al observed the presence of AgNPs of size 24.8-38.6 nm within lettuce leaves when applied through the foliar route.…”

Section: Size-dependent Uptake Of Npsmentioning

confidence: 99%

Nanoparticle-Based Sustainable Agriculture and Food Science: Recent Advances and Future Outlook

Mittal

Kaur

Singh

et al. 2020

Front. Nanotechnol.

326

125

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In the current scenario, it is an urgent requirement to satisfy the nutritional demands of the rapidly growing global population. Using conventional farming, nearly one third of crops get damaged, mainly due to pest infestation, microbial attacks, natural disasters, poor soil quality, and lesser nutrient availability. More innovative technologies are immediately required to overcome these issues. In this regard, nanotechnology has contributed to the agrotechnological revolution that has imminent potential to reform the resilient agricultural system while promising food security. Therefore, nanoparticles are becoming a new-age material to transform modern agricultural practices. The variety of nanoparticle-based formulations, including nano-sized pesticides, herbicides, fungicides, fertilizers, and sensors, have been widely investigated for plant health management and soil improvement. In-depth understanding of plant and nanomaterial interactions opens new avenues toward improving crop practices through increased properties such as disease resistance, crop yield, and nutrient utilization. In this review, we highlight the critical points to address current nanotechnology-based agricultural research that could benefit productivity and food security in future.

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In Vivo Delivery of Nanoparticles into Plant Leaves

Cited by 31 publications

References 40 publications

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Blue light-emitting carbon dots (CDs) from a milk protein and their interaction with Spinacia oleracea leaf cells

Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif

Nanoparticle-Based Sustainable Agriculture and Food Science: Recent Advances and Future Outlook

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