<i>In Vivo</i> Collagen Remodeling in the Vascular Wall of Decellularized Stented Tissue-Engineered Heart Valves

Ghazanfari, Samaneh; Driessen-Mol, Anita Anita; Sanders, Bart; Dijkman, PE Petra; Hoerstrup, SP Simon; Baaijens, Fpt Frank; Bouten, Cvc Carlijn

doi:10.1089/ten.tea.2014.0417

Cited by 35 publications

(28 citation statements)

References 33 publications

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“…material, see www.karger.com/doi/10.1159/000442521). The tortuosity index in the native vascular walls located behind the stent was 0.70 ± 0.03 [Ghazanfari et al, 2015a]. The tortuosity index of the native vascular wall located distal to the stent using the same method was 0.75 ± 0.01, which was significantly different (Student's t test, p < 0.05) from the tortuosity index corresponding to the vascular wall region behind the stent.…”

Section: Remote Control Tissuementioning

confidence: 82%

“…The tortuosity index data of tissueengineered samples were compared with those of native vascular wall tissue located behind the stent [Ghazanfari et al, 2015a]. In short, the tortuosity index was calculated using a Gabor wavelet method, which varied between 0 and 1.…”

Section: Collagen Imaging and Orientation Analysismentioning

confidence: 99%

“…If the tortuosity index was 0, there was no tortuosity, and if the tortuosity index was 1, tortuosity was very high. Using the same method, here we compared the tortuosity index of the native vascular wall behind the stent obtained from the previous study [Ghazanfari et al, 2015a] with the tortuosity index of the native vascular wall located distal to the stent. …”

Section: Collagen Imaging and Orientation Analysismentioning

confidence: 99%

See 2 more Smart Citations

Collagen Matrix Remodeling in Stented Pulmonary Arteries after Transapical Heart Valve Replacement

Ghazanfari¹,

Driessen-Mol²,

Hoerstrup

et al. 2016

Cells Tissues Organs

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The use of valved stents for minimally invasive replacement of semilunar heart valves is expected to change the extracellular matrix and mechanical function of the native artery and may thus impair long-term functionality of the implant. Here we investigate the impact of the stent on matrix remodeling of the pulmonary artery in a sheep model, focusing on matrix composition and collagen (re)orientation of the host tissue. Ovine native pulmonary arteries were harvested 8 (n = 2), 16 (n = 4) and 24 (n = 2) weeks after transapical implantation of self-expandable stented heart valves. Second harmonic generation (SHG) microscopy was used to assess the collagen (re)orientation of fresh tissue samples. The collagen and elastin content was quantified using biochemical assays. SHG microscopy revealed regional differences in collagen organization in all explants. In the adventitial layer of the arterial wall far distal to the stent (considered as the control tissue), we observed wavy collagen fibers oriented in the circumferential direction. These circumferential fibers were more straightened in the adventitial layer located behind the stent. On the luminal side of the wall behind the stent, collagen fibers were aligned along the stent struts and randomly oriented between the struts. Immediately distal to the stent, however, fibers on both the luminal and the adventitial side of the wall were oriented in the axial direction, demonstrating the stent impact on the collagen structure of surrounding arterial tissues. Collagen orientation patterns did not change with implantation time, and biochemical analyses showed no changes in the trend of collagen and elastin content with implantation time or location of the vascular wall. We hypothesize that the collagen fibers on the adventitial side of the arterial wall and behind the stent straighten in response to the arterial stretch caused by oversizing of the stent. However, the collagen organization on the luminal side suggests that stent-induced remodeling is dominated by contact guidance.

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Section: Remote Control Tissuementioning

confidence: 82%

Section: Collagen Imaging and Orientation Analysismentioning

confidence: 99%

Section: Collagen Imaging and Orientation Analysismentioning

confidence: 99%

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Collagen Matrix Remodeling in Stented Pulmonary Arteries after Transapical Heart Valve Replacement

Ghazanfari¹,

Driessen-Mol²,

Hoerstrup

et al. 2016

Cells Tissues Organs

Self Cite

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“…Elastin has been shown in previous studies to be significantly decreased during decellularization in a variety of tissue types (Petersen, Calle et al 2012, Reimer, Syedain et al 2016). However, it has also been observed that the native environment in vivo can restore levels of elastin and other extracellular proteins, such as collagens, after scaffolds are reincorporated into the body (Ghazanfari, Driessen-Mol et al 2015, Reimer, Syedain et al 2016). Alcian Blue staining of decellularized NAC samples confirmed the presence of GAGs, which are an important ECM component for lubrication.…”

Section: Discussionmentioning

confidence: 99%

Characterization of an Acellular Scaffold for a Tissue Engineering Approach to the Nipple-Areolar Complex Reconstruction

Pashos

Scarritt

Eagle

et al. 2017

Cells Tissues Organs

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A significant number of patients undergo mastectomies and breast reconstructions every year using many surgical-based techniques to reconstruct the nipple-areolar complex (NAC). Described herein is a tissue engineering approach that may permit a human NAC onlay graft during breast reconstruction procedures. By applying decellularization, which is the removal of cellular components from tissue, to an intact whole donor NAC, the extracellular matrix (ECM) structure of the NAC is preserved. This creates a biologically derived scaffold for cells to repopulate and regenerate the NAC. A detergent-based decellularization method was used to derive whole NAC scaffolds from nonhuman primate rhesus macaque NAC tissue. Using both histological and quantitative analyses for the native and decellularized tissues, the derived ECM graft was assessed. The bioactivity of the scaffold was evaluated following cell culture with bone marrow-derived mesenchymal stem cells (BMSCs). The data presented here demonstrate that scaffolds are devoid of cells and retain ECM integrity and a high degree of bioactivity. The content of collagen and glycosaminoglycans were not significantly altered by the decellularization process, whereas the elastin content was significantly decreased. The proliferation and apoptosis of seeded BMSCs were found to be approximately 65 and <1.5%, respectively. This study characterizes the successful decellularization of NAC tissue as compared to native NACs based on structural protein composition, lubricating protein retention, the maintenance of adhesion molecules, and bioactivity when reseeded with cells. These histological and quantitative analyses provide the foundation for a novel approach to NAC reconstruction.

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“…These 3D fibrous valve scaffolds support cell culture and matrix remodeling in response to physiologically-relevant flows and pressures when cultured in vitro [240] or in vivo [241]. Implantation of non-endothelialized heart valve constructs into non-human primates resulted in nearly confluent endothelialization after just 4 weeks in vivo [242], suggesting that endothelialization prior to implantation may not be necessary.…”

Section: Cardiac Tissue Repair Using Fibrous Scaffoldsmentioning

confidence: 99%

Fibrous scaffolds for building hearts and heart parts

Capulli

MacQueen

Sheehy

et al. 2016

Advanced Drug Delivery Reviews

111

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Extracellular matrix (ECM) structure and biochemistry provide cell-instructive cues that promote and regulate tissue growth, function, and repair. From a structural perspective, the ECM is a scaffold that guides the self-assembly of cells into distinct functional tissues. The ECM promotes the interaction between individual cells and between different cell types, and increases the strength and resilience of the tissue in mechanically dynamic environments. From a biochemical perspective, factors regulating cell-ECM adhesion have been described and diverse aspects of cell-ECM interactions in health and disease continue to be clarified. Natural ECMs therefore provide excellent design rules for tissue engineering scaffolds. The design of regenerative three-dimensional (3D) engineered scaffolds is informed by the target ECM structure, chemistry, and mechanics, to encourage cell infiltration and tissue genesis. This can be achieved using nanofibrous scaffolds composed of polymers that simultaneously recapitulate 3D ECM architecture, high-fidelity nanoscale topography, and bio-activity. Their high porosity, structural anisotropy, and bio-activity present unique advantages for engineering 3D anisotropic tissues. Here, we use the heart as a case study and examine the potential of ECM-inspired nanofibrous scaffolds for cardiac tissue engineering. We asked: Do we know enough to build a heart? To answer this question, we tabulated structural and functional properties of myocardial and valvular tissues for use as design criteria, reviewed nanofiber manufacturing platforms and assessed their capabilities to produce scaffolds that meet our design criteria. Our knowledge of the anatomy and physiology of the heart, as well as our ability to create synthetic ECM scaffolds have advanced to the point that valve replacement with nanofibrous scaffolds may be achieved in the short term, while myocardial repair requires further study in vitro and in vivo.

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In Vivo Collagen Remodeling in the Vascular Wall of Decellularized Stented Tissue-Engineered Heart Valves

Cited by 35 publications

References 33 publications

Collagen Matrix Remodeling in Stented Pulmonary Arteries after Transapical Heart Valve Replacement

Collagen Matrix Remodeling in Stented Pulmonary Arteries after Transapical Heart Valve Replacement

Characterization of an Acellular Scaffold for a Tissue Engineering Approach to the Nipple-Areolar Complex Reconstruction

Fibrous scaffolds for building hearts and heart parts

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